Eurotium cristatum LS1 strain
A technology of Saccharomyces coronoides and strains, applied in the field of microorganisms, can solve problems such as environmental pollution, chemical fungicides are difficult to decompose, and plant pathogenic bacteria resistance
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Examples
Embodiment 1
[0013] Fu brick tea made from tea leaves collected in Qianyang, Hunan Province has "flowering phenomenon". Pick the golden-yellow closed cysts from Fuzhuan brick tea and inoculate them on PDA medium, culture at a constant temperature of 28°C for 5 days, pick mycelia for streaking on the plate, and repeat the purification 3 times to obtain the strain LS1 of the present invention.
Embodiment 2
[0015] The preparation of spore suspension: prepare the spore suspension of bacterial strain LS1 of the present invention with prior art, concentration is 3 * 10 7 pc / mL, for use.
[0016] Preparation of fermentation broth: the absorption concentration is 3×10 7 Inoculate 1% of the above-mentioned spore suspension per mL onto Pueraria root fermentation medium (2.5 g of Pueraria root and 10 mL of water), and culture at a constant temperature of 28°C. After 7 days, use 95% mass concentration of ethanol to ultrasonically extract the fermented product, take the supernatant, and volatilize it to a constant weight by a rotary evaporator to obtain a fermented product, which is diluted with distilled water to the fermented product to a mass concentration of 0.1 mg / mL.
[0017] Preparation of the unfermented liquid: pueraria puerariae was pulverized into powder, then diluted to 25 mL with distilled water, and the mass concentration of the unfermented liquid was 0.1 mg / mL.
Embodiment 3
[0019] Example 3 Inhibition to pathogenic fungi
[0020] The pathogenic fungi tested: Colletotrichum, Alternaria sonali, Sclerotinia sclerotiorum, and Alternaria brassicae were provided by the Microbiology Laboratory of the School of Food Science and Technology, Hunan Agricultural University.
[0021] Chemical bactericide hymexazol: purchased from Zhengzhou Yukang Biotechnology Company, with an active ingredient content of 80.1%; hymexazol was diluted 1000 times with sterile water and used as a positive control.
[0022] Treatment of treatment groups (Puderaria fermented liquid, Pueraria unfermented liquid) and the positive control group: adopt plate confrontation method, first pour 5mL PDA into a petri dish with a diameter of 90mm, add 2mL treatment group or hymexazol solution after solidification, shake After mixing well, add 15mL PDA. 2 mL of sterile water was added to the culture medium of the blank control group. After solidification, insert 3 mm of pathogenic bacteria ...
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Description
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Application Information
- IPC
- C12N1/14; A01N63/04; A01P1/00; A01P3/00; C12R1/645
- CPC
- A01N63/30; C12N1/145; C12R2001/645
- Inventors
- 易有金; 王璐