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Polygala tenuifolia extract for parkinson's disease and preparation method and application of polygala tenuifolia extract

A Parkinson's disease and extract technology, which is applied in the field of anti-Parkinson's disease Polygala extract and its preparation, can solve problems such as unclear mechanism of action, inability to develop new drugs, and ineffective clinical efficacy of drugs, and achieve broad development and application prospects Effect

Active Publication Date: 2016-08-24
SHANXI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above studies suggest that Polygala has a certain therapeutic effect on PD, but the above research on Polygala anti-PD is based on the in vivo and cellular levels of animals, and its mechanism of action is not clear, and the species of experimental animals and humans are different, which leads to the above research. The clinical curative effect of the screened drugs is not obvious, and their druggability is not high, so they cannot be used in the research and development of new drugs in the later stage

Method used

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  • Polygala tenuifolia extract for parkinson's disease and preparation method and application of polygala tenuifolia extract
  • Polygala tenuifolia extract for parkinson's disease and preparation method and application of polygala tenuifolia extract
  • Polygala tenuifolia extract for parkinson's disease and preparation method and application of polygala tenuifolia extract

Examples

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Effect test

Embodiment 1

[0018] Example 1 Preparation of Polygala extracts and parts

[0019] Weigh 2287g Polygala, cut it to 1-2cm, add 10 times the amount of 95% ethanol to soak overnight, heat and reflux for extraction 3 times, 2 hours each time, combine the extracts, filter, and recover the filtrate under reduced pressure until it has no alcohol smell, to obtain alcohol extract: add 10 times of water to soak the remaining medicinal residue after filtration overnight, heat and reflux for extraction 3 times, each time for 2 hours, combine the extracts, filter, concentrate under reduced pressure to liquid extract, and obtain water extract; The extract and water extract were mixed in equal volumes, 1.5 times the volume of petroleum ether with a boiling point of 60°C-90°C was added, liquid-liquid extraction was performed 3 times, the extracts were collected and combined, the solvent was recovered, concentrated to an extract, and vacuum-dried at 60°C. Pulverize to obtain part A; add 1.5 times the volume...

Embodiment 2

[0020] Example 2 Screening of hDDC Inhibitory Activity of Polygala Extracts and Parts

[0021] 1. Principle of hDDC and PEPC (phosphoenolpyruvate carboxylase) enzyme activity assay

[0022] In order to specifically detect the activity of hDDC, we used an ornithine decarboxylase (ODC) detection method to detect the activity of purified DDC. The principle is based on an enzyme-linked reaction ( figure 2 ), levodopa (L-Dopa) produces dopamine under the action of dopa decarboxylase and releases CO 2 , CO 2 with H 2 O forms HCO 3 — , HCO 3 — It reacts with phosphoenolpyruvate (PPPA) to generate oxaloacetate, and then under the action of malate dehydrogenase (MDH), the consumption of oxaloacetate and nicotinamide adenine dinucleotide (NADH) decreases, with NADH generates non-absorbed NAD as the reaction proceeds + , so the absorbance value at OD340nm decreases. CO produced by deacidification of hDDC 2 There is a 1:1 relationship between the amount of NADH and the amount ...

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Abstract

The invention provides a polygala tenuifolia extract for a parkinson's disease and a preparation method and an application of the polygala tenuifolia extract. The method comprises the following specific steps: taking a polygala tenuifolia material, adding 95% ethanol to soak over the night, carrying out heating reflux extraction and filtration and recovering ethanol until no alcohol taste in a vacuum condition to obtain an alcohol extract; adding water to soak the residual filter residues after filtration over the night, carrying out heating reflux extraction, filtration and vacuum concentration until a liquid extract to obtain a water extract; mixing the alcohol extract and the water extract at equal volume; adding petroleum ether, carrying out extracting and separating to remove the petroleum ether part; adding chloroform to the residual water solution, extracting and collecting a chloroform extracting solution; and concentrating the chloroform extracting solution until an extract, drying the extract in vacuum and crushing the product to obtain the effective part B. Through an in vitro anthropogenic dopa decarboxylase inhibitor screening model, the screened polygala tenuifolia effective part B has obvious anti-PD activity and can be applied to preparation of anti-PD medicines.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to an anti-Parkinson's disease (PD) Polygala extract and a preparation method and application thereof. Background technique [0002] Parkinson's disease (PD) is a common neurodegenerative disease in middle-aged and elderly people. The pathological features of PD are due to the degeneration and loss of a large number of dopaminergic neurons (dopaminergic neurons) in the nigrostriatum of patients, accompanied by the loss of the neurotransmitter dopamine, and the relative increase of acetylcholine, so that the balance between dopamine and acetylcholine cannot be maintained, causing symptoms . Most of the current therapeutic drugs are to maintain the dynamic balance between dopamine and acetylcholine, thereby increasing the content of dopamine in the nigrostriatal pathway of the nerve center. The main reason why oral and intravenous injections cannot supplement the lack of dop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/69A61P25/16
CPCA61K36/69A61K2236/331A61K2236/333A61K2236/35A61K2236/39
Inventor 张福生许晓双张晓瑞邢婕秦雪梅张丽增
Owner SHANXI UNIV
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