Identification method of upland cotton sub-okra leaf germplasm materials and special primers of method
An identification method, a technology of upland cotton, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of affecting the quality of machine-picked cotton, poor management, and shedding of cotton bolls in the middle and lower parts, and achieve The identification results are stable and reliable, the effect of improving selection efficiency and speeding up the breeding process
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[0026] Example 1. A method for identifying germplasm materials of upland gossypii foot leaves, the steps are as follows:
[0027] 1. At the seed or seedling stage, take fresh young leaves of upland cotton material, extract genomic DNA, and test the DNA quality, and dilute to 50ng / ul;
[0028] 2. According to the known cotton EST series, SSRhunter software designed a pair of specific SSR marker primers, forward primer: 5'—GATGCACCAGATCCTTTTAT—3' (SEQ ID No.1); reverse primer: 5'—GGTACATCGGAATCACAGT—3'( SEQ ID No. 2).
[0029] The EST sequence of the designed primer is as follows (SEQ ID No. 3), and the underline indicates the position of the primer.
[0030] CTGGACTAACACCAATAATCACTAAACTTTGATTAAAATAACATTTCAGTTACTAAACTTTCAAAAGTGACAAATCAGTCATTAACATTTACGAAAAGTGACAAATTAGTCACCTGAGAGTGGACATCTGACGTGGCCCGTTAGGGTGCCACGTTGAACATGATCG GATGC ACCAGATCCTTTTAT TAGTTTATAAGGATTACCAACTAAATAGAAGAAGAAGAAGAAGAGGAAGAAGAAGAAGAAAAGGAAGAAGAAGAAGAAGAATAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG...
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[0036] Example 2. Using the method of the present invention to identify 100 samples
[0037] 1. Material:
[0038] Using the normal broad-leaved material Lumianyan 28 as the female parent, and the sub-chicken foot leaf homozygous material S131189 line as the male parent, a sub-chicken foot leaf and normal broad-leaved separation F containing 229 individual plants were constructed. 2 group.
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