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Method for detecting trace BPA (bisphenol A) through nematode dyeing with Nile red

A nematode and trace technology, applied in measurement devices, material analysis by optical means, material excitation analysis, etc., can solve problems such as low detection limit

Inactive Publication Date: 2016-08-24
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of excessive dependence on chemical analysis instruments and low detection limit in existing methods for detecting BPA, and to provide a method for detecting trace BPA by nematode staining using Nile red

Method used

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  • Method for detecting trace BPA (bisphenol A) through nematode dyeing with Nile red

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Experimental program
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specific Embodiment approach 1

[0009] Specific embodiment 1: In this embodiment, the nematode staining method of detecting trace BPA using Nile red is carried out as follows: 1. Weigh 8.01g NaCl, 0.2g KCl, 1.78gNa 2 HPO 4 2H 2 O and 0.27gKH 2 PO 4 After mixing, dilute to 1000mL with distilled water, put it into a high-pressure steam sterilizer at 121°C for 18-22min, take it out and adjust the pH value to 7.4 after cooling to obtain a mixed solution A; 2. Weigh 4-6.5μg Nile red, dissolved in 100ml mixed solution A to obtain mixed solution B; 3. Weigh 13.6g KH 2 PO 4 and 1.8g KOH, after mixing, add distilled water to make the volume to 100mL, and then adjust the pH value to 6.0 to obtain mixed solution C; 4. Add 10mL mixed solution C to the petri dish, and then add 100μL of the liquid to be tested to obtain petri dish A; 5. Add 8-12 eggs of BC10067 mutant nematode to Petri dish A, soak for 4 hours to obtain treated eggs of BC10067 mutant mutant BC10067; 6. Weigh 4-6g of yeast powder, 8-10g of peptone, M...

specific Embodiment approach 2

[0011] Embodiment 2: This embodiment differs from Embodiment 1 in that: the method described in Step 1 is put into a high-pressure steam sterilizer for sterilization at 121° C. for 20 minutes. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0012] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the adjustment of pH value to 7.4 in step 1 is adjusted by sodium hydroxide or hydrochloric acid. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a method for detecting trace BPA (bisphenol A) through nematode dyeing with Nile red and aims to solve the problem of low detection limit of an existing method. The method comprises steps as follows: step one, a mixed solution A is prepared; step two, Nile red is dissolved in the mixed solution A; step three, a mixed solution C is prepared; step four, the mixed solution C and a to-be-measured liquid are added to a culture dish; step five, BC10067 nematode mutant eggs are processed; step six, a nematode culture medium is prepared; step seven, the nematode culture medium is processed; step eight, the BC10067 nematode mutant eggs are cultured and processed, and nematodes are subjected to Nile red luminous intensity detection after the BC10067 nematode mutant eggs grow into adults; if the luminous intensity is higher than 20% of that of detection of the normally growing nematodes, the condition that the to-be-measured liquid contains the endocrine disrupter BPA is determined. With the adoption of the method, the trace BPA detection is realized. The invention belongs to the field of BPA detection.

Description

technical field [0001] The invention relates to a method for detecting trace BPA by nematode staining using Nile red. Background technique [0002] BPA, bisphenol A, is one of the most widely used industrial compounds in the world. In industry, bisphenol A is used to synthesize materials such as polycarbonate (PC) and epoxy resin. In the manufacturing process of plastic products, adding BPA can make it colorless, transparent, durable, lightweight and outstanding impact resistance. BPA is ubiquitous and has become a substance that people are often exposed to. As a typical endocrine disruptor, BPA can lead to endocrine disorders and threaten the health of fetuses and children. Obesity from cancer and metabolic disorders is also thought to be involved. The European Union believes that baby bottles containing bisphenol A can induce precocious puberty. The United States took the lead in banning the use of the chemical substance bisphenol A in food and beverage containers such ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 王云彪
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S