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Prunus mume leaf extract, preparation method and application thereof

A technology of plum leaf extract and plum blossom, which is applied in the field of plant component extraction, can solve the problems of unstable extract composition, complicated component categories, and affecting the application effect, so as to protect skin cells, inhibit the formation of pigmentation, and maintain vitality Effect

Inactive Publication Date: 2016-08-31
SHANGHAI SHENYIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Plum blossom leaves are produced at the turn of February and March each year, generally later than the flowering period. Plum blossom leaves are rich in quercetin, isorhamnetin and phytoglycosides. In the prior art, when plum blossoms are extracted, ultrasound-assisted extraction is often used. Low frequency (10-20KHz) and medium frequency (40kHz) are used, alcohol solvents (such as butanediol-acetone system) are added to the eluent during separation, the eluent system is more polar, and the component types of the extract Complicated and mostly volatile substances, the composition of the extract is unstable, affecting the application effect, and the application is limited

Method used

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  • Prunus mume leaf extract, preparation method and application thereof
  • Prunus mume leaf extract, preparation method and application thereof
  • Prunus mume leaf extract, preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1 A kind of preparation method of plum blossom leaf extract, comprises the following steps:

[0034] 1) Wash, chop and grind 50g of plum blossom leaves;

[0035] 2) Add 1L of ethanol at a temperature of 50°C, keep the mass ratio of plum blossom leaves and ethanol at 1:10-50, and stir for 30 minutes under sealed and dark conditions;

[0036] 3) Filtrate, collect the filtrate, keep the filter residue, evaporate and concentrate under reduced pressure, and obtain an ethanol solution of 500 milliliters of plum blossom leaf extract;

[0037] 4) Add 100mL 1,2-butanediol, add the filter residue, extract again under ultrasonic conditions at room temperature for 1.5h, evaporate under reduced pressure, filter, use silica gel column to purify twice, and use hydroxypropyl dextran gel column chromatography Purify twice, gradient elution, the volume ratio of petroleum ether and acetone is from 100:0 to 100:14, collect the eluate when the volume ratio of petroleum ether and...

Embodiment 2

[0039] Hydroxyproline is an important amino acid with stable content (accounting for 13.4%) in collagen, and its content is very small in elastin, and it does not exist in other proteins. Therefore, the determination of its content is to detect the synthesis ability of collagen A reliable method, the detection of hydroxyproline includes the following steps:

[0040] 1. Preparation of Human Skin Cells

[0041] Take normal human dermal fibroblasts as primary cells, and place them in a 75cm 2 In a culture flask (2ml of culture medium containing growth factors), 37°C, CO 2 The cells were cultured to 80% concentration under the condition of 5% concentration, and passaged, the passaged cells were 175cm 2 Continue culturing the cells in the square flask to a concentration of 80% to obtain human dermal fibroblasts.

[0042] 2. Preparation of samples to be tested

[0043]Preparation of essence containing plum leaf extract, essence ingredients: glycerin 5.0wt.%, butanediol 4.0wt.%, ...

Embodiment 3

[0060] The hyaluronic acid content determination is carried out to the culture supernatant of the A sample, B sample, C sample, D sample and control group sample obtained in step 2 of Example 2, comprising the following steps:

[0061] 1. Draw hyaluronic acid standard curve

[0062] Prepare 6 hyaluronic acid standard solutions, the concentrations are: 0μg / mL, 10μg / mL, 20μg / mL, 40μg / mL, 80μg / mL, 160μg / mL, and test their OD at 450nm with a microplate reader value, the test results are shown in Table 3;

[0063] table 3

[0064] Hyaluronic acid standard (μg / mL)

[0065] According to the test results of hyaluronic acid standard products, the absorbance is taken as the vertical axis, and the hyaluronic acid concentration is taken as the horizontal axis to draw the standard curve of hyaluronic acid, see image 3 ;OD=0.0156×c+0.2083,R 2 =0.9841, p=0.0001, wherein, c is the concentration of hyaluronic acid, unit μg / mL, after conversion, the concentration of hyaluronic aci...

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Abstract

The invention provides a prunus mume leaf extract, a preparation method and application thereof. The prunus mume leaf extract is prepared from, by weight, 0.5-10.0% of quercetin, 0.5-5.0% of isorhamnetin, 50.0-70.0% of plant glucoside, 5-10% of quercetin derivative and isorhamnetin derivative and inevitable impurities. Prunus mume leaves are extracted through the method combining ethanol extraction and ultrasonic extraction, ethanol at 40-65 DEG C is used for extraction so that solubility of quercetin and isorhamnetin can be improved, the dissolving-out speed increased due to ultrasonic extraction, and accordingly dissolution is more thorough. The prunus mume leaf extract is a pure natural component and has the functions of reducing ultraviolet radiation, preventing oxidation and the like, and accordingly skin cells are protected. The extract can also have the function of preventing skin acne generation, reduces pigmentation, inhibits stain generation, and makes skin white and bright.

Description

technical field [0001] The invention belongs to the field of plant component extraction, and in particular relates to a plum blossom leaf extract, its preparation method and application. Background technique [0002] Plum blossom is a deciduous tree of the genus Prunus in the family Rosaceae, a rare shrub; the branchlets are green, smooth and hairless; the leaves are ovate or elliptic, and the plum blossom is cold and heat-resistant, whether it is in a low temperature environment of -10°C or in a high temperature of 40°C. Can survive very well. [0003] Plum blossom is native to the south of my country and is one of the traditional famous flowers in China. It was designated as the national flower of China in 1929. As a traditional flower in China, plum blossom is also one of the iconic flowers. It mostly means innocence and excellence. Zhou Bangyan, a poet in the Song Dynasty, has a word saying: "One cut plum blossom is ten thousand and delicate, and the branches are insert...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61K8/49A61K8/60A61Q9/02A61Q19/08A61Q19/00
CPCA61K8/498A61K8/602A61K8/97A61Q9/02A61Q19/00A61Q19/08
Inventor 朱剑虹王智富朱侗明
Owner SHANGHAI SHENYIN BIOTECH
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