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Chestnut shell extract as well as preparation method and application thereof

A technology of extract and chestnut shell, applied in the field of chestnut shell extract and preparation thereof, can solve the problems of difficult to penetrate cell membranes, difficult drug candidate compounds, etc., and achieve the effect of strong operability

Inactive Publication Date: 2016-08-31
郭传明 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the research on PTP1B (protein tyrosine phosphatase 1B) selective inhibitors has made some progress, but most of the research is limited to peptides and peptidoids, for example: according to the substrate sequence of PTP1B dephosphorylation design Inhibitors EEDE(F2PMP)M, Glu-F2PMP-F2PMP, although these peptide compounds have high selectivity and strong inhibitory activity, but they are not easy to penetrate the cell membrane, and the structure of peptide phosphate makes it difficult to become drug candidates

Method used

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  • Chestnut shell extract as well as preparation method and application thereof
  • Chestnut shell extract as well as preparation method and application thereof
  • Chestnut shell extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of chestnut shell extract is prepared by the following method:

[0035] 1. Extraction.

[0036] With the dried and chopped Chinese chestnut (Castanea mollissima Blume) shell, use volume percent concentration as 100% ethanol (i.e. pure ethanol), according to the chestnut shell and ethanol solid-liquid ratio (g / ml) is 5-10 times the amount, Perform ultrasonic extraction 2-4 times, 30min-60min each time, and combine the extracts. In this example, 500 g of chestnut shells and 5 L of ethanol were subjected to ultrasonic extraction for 3 times, 60 min each time.

[0037] Subsequently, the extract was concentrated under reduced pressure to recover ethanol until it was more difficult to concentrate, the concentrated solution was combined, continued to heat in an open container to evaporate the ethanol as much as possible, let it cool, and obtained 32g of 100% ethanol extract, and obtained the chestnut shell extract after drying , which is component I.

[0038] 2. Puri...

Embodiment 2

[0041] A kind of chestnut shell extract is prepared by the following method:

[0042] 1. Extraction.

[0043] The dried and chopped chestnut (Castanea mollissima Blume) shells were subjected to ultrasonic extraction with 75% ethanol aqueous solution according to the chestnut shell and ethanol solid-liquid ratio (g / ml) as 5-10 times. - 4 times, 30min-60min each time, combine the extracts. In this example, 500 g of chestnut shells and 5 L of ethanol were subjected to ultrasonic extraction for 3 times, 60 min each time.

[0044] Subsequently, the extract was concentrated under reduced pressure to recover ethanol until it was more difficult to concentrate, the concentrated solution was combined, continued to heat in an open container to evaporate the ethanol as much as possible, let it cool, and obtained 38.2g of 100% ethanol extract, which was dried to obtain chestnut shell extract material, which is component II.

[0045] 2. Purification.

[0046] The chestnut shell extract ...

Embodiment 3

[0049] A kind of chestnut shell extract is prepared by the following method:

[0050] 1. Extraction.

[0051] The dried and chopped Chinese chestnut (Castanea mollissima Blume) shell is subjected to ultrasonic extraction with 50% ethanol water solution according to the chestnut shell and ethanol solid-liquid ratio (g / ml) as 5-10 times. - 4 times, 30min-60min each time, combine the extracts. In this example, 500 g of chestnut shells and 5 L of ethanol were subjected to ultrasonic extraction for 3 times, 60 min each time.

[0052] Subsequently, the extract was concentrated under reduced pressure to recover ethanol until it was more difficult to concentrate, the concentrated solution was combined, continued to heat in an open container to evaporate the ethanol as much as possible, let it cool, and obtained 28.5g of 100% ethanol extract, which was dried to obtain chestnut shell extract matter, which is component III.

[0053] 2. Purification.

[0054] The chestnut shell extrac...

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Abstract

The invention relates to a chestnut shell extract as well as a preparation method and application thereof, and belongs to the technical field of natural product extraction and application. The preparation method comprises the following steps of: extraction, namely performing solvent extraction by using chestnut shells as a raw material and using a 40-100% ethanol aqueous solution as a solvent, and recovering the solvent, thus obtaining the chestnut shell extract. The chestnut shell extract has the effect of inhibiting protein tyrosine phosphatases 1B (PTP1B), and the extract can be used as a PTP1B inhibitor for treating diabetes, obesity and other complications.

Description

technical field [0001] The invention relates to the technical field of extraction and application of natural products, in particular to a chestnut shell extract and its preparation method and application. Background technique [0002] At present, clinically, diabetes is often divided into two types: insulin-dependent (IDDM, type I diabetes) and non-insulin-dependent (NIDDM, type II diabetes), of which type II diabetes accounts for 90% of diabetes. WHO predicts that the number of people with diabetes will rise from 135 million in 1995 to 300 million by 2025 due to aging population, obesity, unhealthy diet and inactive lifestyle. [0003] Type 2 diabetes is characterized by resistance to the action of insulin in insulin-sensitive tissues such as skeletal muscle, liver, and adipose tissue. Although the specific mechanism is unclear, the weakening or even blocking of insulin signaling in its transduction pathway must be directly related. [0004] PTPases (protein tyrosine phos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P3/10A61P3/04A61P5/50
CPCA61K36/185A61K2236/333A61K2236/39A61K2236/55
Inventor 郭传明崔龙
Owner 郭传明
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