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A class of furostanol saponins and their applications

A technology of furostanol saponins and compounds, which is applied in the field of furostanol saponins, and can solve problems such as insufficient research on the chemical composition of open arrows

Active Publication Date: 2017-12-12
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing research on the chemical composition of open arrow is still not thorough enough, so the chemical composition of steroids in open arrow is worthy of further research and development

Method used

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  • A class of furostanol saponins and their applications
  • A class of furostanol saponins and their applications
  • A class of furostanol saponins and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The extraction and identification of embodiment 1 furostanol saponins

[0027] 1. Extraction of furostanol saponins

[0028] Take 17 kg of open arrow rhizomes, heat and reflux with 60% ethanol to extract 4 times, each time for 4 hours, combine the extracts to recover the solvent under reduced pressure, and obtain the total extract, suspend the total extract with water, and extract with equal volume of ethyl acetate for 4 Next, fat-soluble impurities were removed, and the aqueous layer was separated by D101 macroporous resin column chromatography, and eluted with water, 20% ethanol, 60% ethanol, 80% ethanol, and 95% ethanol respectively to obtain 5 fractions TA, TB, TC, TD, TE. Fraction TC (60% ethanol eluted part) 200g was taken, and the compounds 1, 2 and 6 of the present invention were isolated by silica gel column chromatography, ODS medium and low pressure column chromatography, reverse phase high performance liquid chromatography and other separation methods. Usi...

Embodiment 3

[0113] Embodiment 3 anti-inflammatory test:

[0114] For the anti-inflammatory activity test of the compound of the present invention in vitro, Raw 264.7 (mouse macrophage) cells induced by LPS (lipopolysaccharide) were used to establish an in vitro inflammation model. MTT and Griess experiments were used to investigate the effect of the compound of the present invention on the release of inflammatory mediator NO from Raw 264.7 cells induced by lipopolysaccharide. The anti-inflammatory drug Indomethacin (indomethacin) was used as a positive control.

[0115] 1) MTT experiment

[0116] Raw 264.7 cells were inoculated in a 96-well plate, and after 24 hours of culture, the product to be tested was added, and after another 24 hours of culture, the inhibition rate of the sample on tumor cell proliferation was measured by the MTT method. The cell proliferation inhibition rate was calculated according to the following formula, and the half inhibitory concentration (IC) of the teste...

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Abstract

The invention discloses a class of furostanol saponins and applications thereof. The furostanoid saponins are extracted and separated from the open arrow rhizomes. Through experimental research, it is found that the 10 furostanoid saponins obtained in the present invention have good effects in preventing and treating cancer and anti-inflammation. Pharyngeal cancer, etc. have a certain inhibitory effect. Moreover, the compounds extracted from plants have the advantages of high efficiency and low toxicity, and are expected to be developed as new anticancer drugs and health food.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a class of furostanol saponins and applications thereof. Background technique [0002] Tumor is a new organism characterized by excessive cell proliferation produced by the cells of human organs and tissues under the long-term action of external and internal harmful factors. It can be divided into two categories: benign tumors and malignant tumors in medicine. Benign tumors have little impact on human health, while malignant tumors (also known as cancer) seriously threaten human health. According to the latest statistics from the World Health Organization and the American Cancer Society, cancer has become the leading cause of human death. In 2008, there were about 12.7 million newly diagnosed cancer cases and 7.6 million cancer deaths worldwide (accounting for about 13% of all deaths) , and approximately 70% of cancer deaths occur in low- and middle-income countries...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00A61P35/00A61P35/02A61P29/00
CPCC07J71/0005
Inventor 何祥久向丽敏王宜海易晓敏
Owner GUANGDONG PHARMA UNIV
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