Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of phycobiliprotein in preparation of photosensitizer for treating oral and pharyngeal cancer cell by using iodine-tungsten lamp

The technology of phycobiliprotein and iodine tungsten lamp is applied in the field of clinical medicine in medical oncology, can solve problems such as skin phototoxicity, and achieve the effects of low price, low cost of light source and low price

Inactive Publication Date: 2010-06-30
SHANGHAI OCEAN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional photosensitizers have serious skin phototoxicity and other shortcomings, and the development of new photosensitizers has become a research hotspot

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of phycobiliprotein in preparation of photosensitizer for treating oral and pharyngeal cancer cell by using iodine-tungsten lamp
  • Application of phycobiliprotein in preparation of photosensitizer for treating oral and pharyngeal cancer cell by using iodine-tungsten lamp
  • Application of phycobiliprotein in preparation of photosensitizer for treating oral and pharyngeal cancer cell by using iodine-tungsten lamp

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] The materials used in this example are Porphyra phycoerythrin R-PE and throat cancer Hep-2 cell line, wherein Porphyria phycoerythrin R-PE was purchased from Hangzhou Aowei Bioengineering Co., Ltd., and throat cancer Hep-2 2 Cell lines were purchased from the Institute of Cells, Chinese Academy of Sciences.

[0009] (1) Throat cancer Hep-2 cell line was cultured with 1640 culture medium containing 10% calf serum, and the culture conditions were 37°C and 5% CO 2 concentration and saturation humidity;

[0010] (2), when the cells grow vigorously, digest the cells with 0.25% trypsin containing 0.02% ethylenediaminetetraacetic acid (EDTA), wash and blow off the cells and dilute to 10 6 / ml, 100 μL per well was inoculated in a 96-well plate;

[0011] (3), with a concentration of 100 μg / mL and a purity of 5.5 (A 564nm / A 280nm ) Serum-free culture medium of Porphyra phycoerythrin R-PE to replace the above-mentioned culture solution, cultivated for 4 hours;

[0012] (4) I...

Embodiment 2

[0017] The materials used in this example are Porphyra phycocyanin C-PC and throat cancer Hep-2 cell line, wherein Porphyra phycocyanin C-PC was purchased from Hangzhou Aowei Bioengineering Co., Ltd., and throat cancer Hep-2 2 Cell lines were purchased from the Institute of Cells, Chinese Academy of Sciences.

[0018] (1), with a purity of 4.0 (A 615nm / A 280nm ) Laver phycocyanin C-PC replaces Porphyrin phycoerythrin R-PE to do each step of (1)-(6) in Example 1;

[0019] (2) The results showed that the killing rate of Porphyrocyanin C-PC at a concentration of 100 μg / mL to throat cancer Hep-2 cells was 66%.

Embodiment 3

[0021] Gradient comparison steps:

[0022] 1. Tumor cell culture

[0023] Pharyngeal carcinoma Hep-2 cell line was cultured in 1640 medium containing 10% calf serum at 37°C, 5% CO 2 Concentration and saturated humidity, when the cells grow vigorously, digest the cells with 0.25% trypsin containing 0.02% ethylenediaminetetraacetic acid (EDTA), wash and blow off the cells and dilute to 10 6 / mL, 100 μL per well was inoculated in a 96-well plate for photodynamic reaction.

[0024] 2. Effect of Porphyrophycoerythrin R-PE and Porphyrocyanin C-PC-mediated photodynamic response on the survival rate of throat cancer Hep-2 cells

[0025] The culture medium with different final concentrations (10, 25, 50, 100 μg / mL) of Porphyra phycoerythrin R-PE and Porphyra phycocyanin C-PC were respectively replaced with the original medium. Tungsten lamp irradiation, when irradiating with iodine tungsten lamp, an insulated water tank is added between the irradiated sample and the light source to ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the application of phycobiliprotein in preparation of photosensitizer for treating oral and pharyngeal cancer cell by using an iodine-tungsten lamp. The phycobiliprotein of the invention is selected from porphyra yezoensis phycoerythrin R-PE and porphyra yezoensis phycocyanin C-PC extracted from porphyra yezoensis; under the light of the iodine-tungsten lamp, the effective photosensitizer has killing effect to the Hep-2 cells of the oral and pharyngeal cancer and high killing ability. The raw material is abundant in source and cheap in price; the extract process is simple; the iodine-tungsten lamp as the light source is small in volume and cheap in price and has low cost of the light source; in brief, the porphyra yezoensis phycoerythrin R-PE or the porphyra yezoensis phycocyanin C-PC as the photosensitizer kills the Hep-2 cells of the oral and pharyngeal cancer under the light of the iodine-tungsten lamp, greatly reduces the use cost in the course of the photodynamic therapy and is beneficial to wide popularization and use.

Description

Technical field: [0001] The invention relates to a medicine for clinical use in medical oncology, in particular to the application of a phycobiliprotein in the preparation of a photosensitizer for treating throat cancer cells with an iodine-tungsten lamp. Background technique: [0002] Photodynamic therapy is a new type of tumor therapy established and developed in the past 20 years. Its principle is mainly to achieve the killing effect on tumor cells by enriching the photosensitizer in the lesion and producing photosensitive damage under light. Photodynamic therapy depends largely on the nature of the photosensitizer employed. Traditional photosensitizers have serious skin phototoxicity and other shortcomings, and the development of new photosensitizers has become a research hotspot. Phycobiliprotein is a good pure natural pigment, which can be used in food, cosmetics, fluorescence detection and other fields. In recent years, according to the characteristics of its absorp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/16A61K41/00A61P35/00
Inventor 何培民蔡春尔王源李春霞汪卿柳俊秀
Owner SHANGHAI OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com