Molecular marker for identifying early blossoming/late blossoming of lichee in early stage and applications thereof

A molecular marker, lychee technology, applied in the field of plant molecular genetics, can solve the problems of long hybrid breeding cycle, large area, labor and time-consuming, etc., and achieve the effect of shortening the breeding cycle, rapid and accurate screening, and wide application prospects.

Active Publication Date: 2016-09-07
广西壮族自治区农业科学院园艺研究所
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Litchi is a woody fruit tree with a tall tree body and a long childhood perio

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker for identifying early blossoming/late blossoming of lichee in early stage and applications thereof
  • Molecular marker for identifying early blossoming/late blossoming of lichee in early stage and applications thereof
  • Molecular marker for identifying early blossoming/late blossoming of lichee in early stage and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0053] Example 1 : A method for rapid and qualitative identification of early-flowering and late-flowering litchi germplasm resources by molecular markers

[0054] (1) Design of primers

[0055] Based on the difference of the LcFT1 gene promoter sequences of early-flowering lychee and late-flowering litchi, and according to the specificity of the LcFT1 gene promoter sequence of late-flowering lychee, two pairs of upstream and downstream primer pairs were designed (see Table 1), and the molecular marker ZW1 and molecular marker were obtained respectively. ZW2.

[0056] Table 1 Primer information

[0057]

[0058] Note: ZW1-1 and ZW1-2 primer pairs are used to detect whether LcFT1 gene is contained in late-flowering litchi; ZW2-1 and ZW2-2 primer pairs are used to detect whether early-flowering litchi contains LcFT1 gene.

[0059] (2) Extraction of genomic DNA of lychee to be detected

[0060] Select 16 healthy leaves of different early-flowering and late-flowering litch...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of plant molecular genetics, and specifically relates to a molecular marker for identifying early blossoming/late blossoming of lichee in the early stage and applications thereof. The molecular marker is characterized by comprising ZW1 and ZW2. The molecular marker ZW1 is prepared by amplifying an upstream primer represented by Seq ID No.1 and a downstream primer represented by Seq ID No.2. The molecular marker ZW2 is prepared by amplifying an upstream primer represented by Seq ID No.3 and a downstream primer represented by Seq ID No.4. The provided molecular marker and identification method have the advantages that the screening is rapid and accurate, the identification can be carried out in the early stage of seedlings, the identification method has an important meaning for breeding lichee species with the characteristics of early blossom and late blossom, the breeding period is prominently shortened, labor, materials, and land are largely saved, high economic and social benefits are generated, and the identification method has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant molecular genetics, and in particular relates to a molecular marker of a key gene LcFT1 promoter determining the early or late flowering of litchi and the application of the molecular marker in hybrid breeding of litchi maturity. Background technique [0002] Litchi (Litchi chinensis Sonn.) is a Sapindaceae plant of the genus Litchi, which originated in China. It is known as the "Lingnan Fruit King" because of its fruit shape, color, aroma, taste and rich nutrition. Litchi planting in China has formed a large-scale commercial production, and its output ranks first in the world. Past production experience shows that early-flowering varieties (such as 'Sanyuehong') only need a short period of low temperature to complete flower induction (in the middle and late October), so they are easy to flower, and generally bloom around February, while late Flower varieties (such as 'Maguili') must undergo low te...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68C12N15/11
Inventor 丁峰彭宏祥张树伟朱建华秦献泉
Owner 广西壮族自治区农业科学院园艺研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap