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Molecular marker for identifying early blossoming/late blossoming of lichee in early stage and applications thereof

A molecular marker, lychee technology, applied in the field of plant molecular genetics, can solve the problems of long hybrid breeding cycle, large area, labor and time-consuming, etc., and achieve the effect of shortening the breeding cycle, rapid and accurate screening, and wide application prospects.

Active Publication Date: 2016-09-07
广西壮族自治区农业科学院园艺研究所
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Problems solved by technology

Litchi is a woody fruit tree with a tall tree body and a long childhood period, resulting in a long hybrid breeding cycle, a large area, and time-consuming

Method used

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  • Molecular marker for identifying early blossoming/late blossoming of lichee in early stage and applications thereof
  • Molecular marker for identifying early blossoming/late blossoming of lichee in early stage and applications thereof
  • Molecular marker for identifying early blossoming/late blossoming of lichee in early stage and applications thereof

Examples

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Embodiment 1

[0053] Example 1 : A method for rapid qualitative identification of early and late flowering litchi germplasm resources by molecular markers

[0054] (1) Design of primers

[0055] Based on the differences in the promoter sequences of the LcFT1 gene in early-flowering and late-flowering litchi, and according to the specificity of the promoter sequences of the LcFT1 gene in late-flowering and early-flowering litchi, two pairs of upstream and downstream primers were designed (see Table 1), and molecular markers ZW1 and molecular markers were obtained, respectively. ZW2.

[0056] Table 1 Primer information

[0057]

[0058] Note: The primer pair ZW1-1 and ZW1-2 are used to detect whether the LcFT1 gene is contained in the late-flowering litchi; the primer pair ZW2-1 and ZW2-2 are used to detect whether the LcFT1 gene is contained in the early-flowering litchi.

[0059] (2) Extraction of Litchi Genomic DNA to be detected

[0060] Select 16 healthy leaves of different early...

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Abstract

The invention belongs to the technical field of plant molecular genetics, and specifically relates to a molecular marker for identifying early blossoming / late blossoming of lichee in the early stage and applications thereof. The molecular marker is characterized by comprising ZW1 and ZW2. The molecular marker ZW1 is prepared by amplifying an upstream primer represented by Seq ID No.1 and a downstream primer represented by Seq ID No.2. The molecular marker ZW2 is prepared by amplifying an upstream primer represented by Seq ID No.3 and a downstream primer represented by Seq ID No.4. The provided molecular marker and identification method have the advantages that the screening is rapid and accurate, the identification can be carried out in the early stage of seedlings, the identification method has an important meaning for breeding lichee species with the characteristics of early blossom and late blossom, the breeding period is prominently shortened, labor, materials, and land are largely saved, high economic and social benefits are generated, and the identification method has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant molecular genetics, and in particular relates to a molecular marker of a key gene LcFT1 promoter determining the early or late flowering of litchi and the application of the molecular marker in hybrid breeding of litchi maturity. Background technique [0002] Litchi (Litchi chinensis Sonn.) is a Sapindaceae plant of the genus Litchi, which originated in China. It is known as the "Lingnan Fruit King" because of its fruit shape, color, aroma, taste and rich nutrition. Litchi planting in China has formed a large-scale commercial production, and its output ranks first in the world. Past production experience shows that early-flowering varieties (such as 'Sanyuehong') only need a short period of low temperature to complete flower induction (in the middle and late October), so they are easy to flower, and generally bloom around February, while late Flower varieties (such as 'Maguili') must undergo low te...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 丁峰彭宏祥张树伟朱建华秦献泉
Owner 广西壮族自治区农业科学院园艺研究所
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