High-throughput microorganism isolation culture method
A technology for separation and cultivation of microorganisms, applied in the field of high-throughput separation and cultivation of microorganisms, which can solve the problems of increasing separation efficiency and "pollution" of dominant bacteria in the cultivation system, and achieve the effects of increasing separation efficiency, making full use of it, and improving utilization
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[0033] 1) Preparation of bacterial suspension
[0034] The samples of polymetallic nodules were collected from station PRZ1302-MC12 (154°49.8218′W, 10°29.2573′N) in the Eastern Pacific Nodule Area in 2013, with a water depth of 5098m. After the sampler obtains the sample, immediately put it into a sterile sealed bag and store it in the dark at 4°C. In the laboratory, take a piece of tuberculosis sample (diameter 1 ~ 2cm), put it into a 15ml chromatography column, add sterile filtered seawater (supplemented with 1mM NH 4 Cl), 10 ℃ dark enrichment for 6 months, every month with 7ml of fresh sterile seawater (supplemented with 1mM NH 4 C1) replace the culture medium. After the enrichment culture was completed, 1 ml of the culture solution was taken and filtered onto a 0.22 μm microporous membrane for microbial cell counting. According to the counting results, the concentration obtained by concentration of the sample is about 10 7 cells / ml bacterial suspension. Carry out doub...
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