Chlorophyll content detection method insensitive to leaf surface structure
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A technology of chlorophyll content and detection method is applied in the detection field of chlorophyll content to achieve the effect of non-destructive testing
Active Publication Date: 2016-09-21
PEKING UNIV SHENZHEN GRADUATE SCHOOL
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However, few researchers have tried to evaluate the estimation accuracy of vegetation index on chlorophyll conten
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Embodiment 1
[0036] This embodiment discloses a method for detecting chlorophyll content that is insensitive to leaf surface structure, and is characterized in that the method includes the following steps:
[0037] 1) Obtain the hyperspectral reflectance of the plant leaf surface, and measure the chlorophyll content of the plant leaf:
[0038] 1-1) Using ASD 3 The leaf clip of the portable spectral measuring instrument (spectral analyzer, Boulder, CO, USA), collects the spectral reflection information of the front and back of the leaf within the wavelength range of 400-1000nm. During the collection process, try to select the same part for all leaves to avoid measuring the veins. Each leaf is measured three times, and a whiteboard calibration is performed every 5 minutes;
[0039] In 1-2), a hole puncher with a diameter of 6 mm was used to take out the leaf area where the reflectance of the leaf was measured, and five holes were punched for each leaf. Put the punched leaves into a mortar,...
Embodiment 2
[0060] In this example, fifty-five leaves of A. chinensis, tung flower tree and Sangria apetalum were collected in the mangrove forest, and their spectral reflectance and chlorophyll content were measured to develop a new chlorophyll index that is not affected by leaf surface information.
[0061] Overall, such as figure 1 As shown, the present embodiment discloses a method for detecting chlorophyll content insensitive to leaf surface structure, said method comprising the steps of:
[0062] 1) Obtain the hyperspectral reflectance of the plant leaf surface, and measure the chlorophyll content of the plant leaf;
[0063] 2) Analyze the correlation between all the band combination results of the nine types of vegetation indices and the chlorophyll content;
[0064] 3) Determine the most effective vegetation index for predicting chlorophyll content according to the correlation coefficient (r) and root mean square error (RMSE);
[0065] 4) Establish a regression equation between ...
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Abstract
The invention discloses a chlorophyll content detection method insensitive to a leaf surface structure, and relates to the technical field of methods for measuring the chlorophyll content through an optical means. The method comprises the steps that hyperspectral reflectance of the plant leaf surface is obtained; the chlorophyll content is measured; a regression equation is established for nine types of vegetation indexes and the chlorophyll content, and a correlation coefficient and a root mean square error are calculated; a regression equation is established for 36 types of published vegetation indexes and the chlorophyll content, and a correlation coefficient and a root mean square error are calculated; the wave bands which are most sensitive and least sensitive to a change of the chlorophyll content are determined; a vegetation index type which is least sensitive to the leaf surface structure in the nine types of the vegetation indexes is determined; new vegetation indexes estimating the chlorophyll content are developed based on spectral information of the front and back of leaves, and the new vegetation indexes which are not sensitive to the leaf surface structure are used for detecting the chlorophyll content of the plant leaves. By means of the chlorophyll content detection method insensitive to the leaf surface structure, the content of chlorophyll in the plant leaves can be detected rapidly, accurately and nondestructively.
Description
technical field [0001] The invention relates to the technical field of methods for measuring chlorophyll content by optical means, in particular to a method for detecting chlorophyll content that is insensitive to leaf surface structure. Background technique [0002] Mangrove is a coastal habitat with important ecological significance. It is a special vegetation that grows in the intertidal zone of tropical and subtropical coasts. This biome. Mangroves grow widely in bays and estuaries along the southeast coast of my country, and are naturally distributed in Hainan, Guangdong, Guangxi, Fujian, Taiwan, Hong Kong and Macau. The Futian mangrove forest in Shenzhen Bay is the only mangrove ecosystem in the center of the city in my country, which is of great significance to the local ecological and economic development. However, due to human disturbance and climate change, Futian mangroves are in decline, and the current distribution area is only 369ha. The Shenzhen Municipal G...
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