Rapid detection method of tobacco leaf volatile components

Abstract

A rapid detection method of tobacco leaf volatile components is characterized in that the method adopts dichloromethane for extraction of tobacco leaves, a sample after extraction is separated by a chromatographic column, a tail end shunt of the chromatographic column is regulated by using a micro plate flow path control technology, at the same time, the shunt enters a quadrupole time-of-flight mass spectrometer (QTOF MS) and a flame ionization detector (FID) and is determined, accurate qualitation and quantitation of the tobacco leaf volatile components is achieved, the whole process has the advantages of simple operation and short use time, and mass and automatic processing of the sample is achieved. The process can be completed by only requiring a simple solvent for extraction, a small amount of an organic solvent is consumed, the whole process has no second-order reaction problem of the tobacco leaf volatile components, and actual conditions of the sample is really reflected; an extract liquid is detected by using gas chromatography-quadrupole time-of-flight mass spectrometry / flame ionization detection for a first time, only one-needle sample injection is needed, and a QTOF MS spectrogram and an FID spectrogram are obtained, wherein QTOF MS can perform accurate quantitation of components of complex samples while the FID can perform accurate quantification of samples with wide concentration scope, and the retaining time of the QTOF MS spectrogram and the retaining time of the FID spectrogram are just the same.

Description

technical field [0001] The invention belongs to the technical field of measuring volatile components of tobacco leaves, and in particular relates to a rapid detection method for volatile components of tobacco leaves. Background technique [0002] The volatile components of tobacco leaves are important components of tobacco leaves, play a major role in the aroma of tobacco, and are an important factor in evaluating the grade of tobacco leaves. At present, there are many methods to report the analysis of the volatile components of tobacco leaves, usually using extraction techniques such as steam distillation, simultaneous distillation extraction and accelerated solvent extraction, the volatile components in the extracted tobacco leaves are analyzed by gas chromatography-mass spectrometry (GC-MS) Determination. The current pretreatment methods all have high extraction temperature, prone to secondary reaction problems, cannot truly reflect the actual volatile components in the ...

AI Technical Summary

Problems solved by technology

[0005] At present, two instruments are usually used for the above analysis, that is, a GC-QTOF MS instrument and a gas chromatograph equipped with an FID, or a chromatograph equipped with an FID coupled with a QTOF MS instrument. Among them, the chromatograph is equipped with two injection ports and connected to two columns. The above analysis methods all have the problem of inconsistency in the retention time of the mass spectrum signal and the FID signal of the same substance, which affects the accurate analysis.

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