Green organic selenium-rich feed and preparation method thereof
A technology rich in organic selenium and organic selenium, applied in the field of green organic selenium-rich feed and its preparation, can solve the problems of incomplete microbial probiotic formula, unstable production of feed products, etc., so as to improve palatability, reduce content, and promote growth. Effect
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[0092] The preparation method of the whole gynostemma powder is as follows: puffing the dried whole gynostemma with an extruder at normal temperature, passing through a 60-mesh sieve after being pulverized, and set aside.
[0093] The isolation and identification method of Bacillus subtilis SE201412 is as follows:
[0094]Separation: Take 1g of the material from the fermented tobacco waste of the Salt Rebaking Factory in Enshi City, Hubei Province, and dilute it with sterile water for 10 g on a sterile ultra-clean workbench. -1 -10 -8 , use a pipette to take 0.1ml-0.2ml into the sterilized special medium (1000ml of tap water, 5g of selenium (provided by sodium selenite), 20g of glucose, 15g of peptone, 5g of sodium chloride, 0.5g of beef extract liter, yeast extract 0.25%, dipotassium hydrogen phosphate 0.1%, PH 7.5), spread evenly with a spreader rod, aseptic culture, and choose a single colony that grows vigorously on the high-selenium medium.
[0095] Identification: Afte...
Embodiment 1
[0113] A preparation method of green feed rich in organic selenium, comprising the steps of:
[0114] (1) Preparation of fermentation raw materials: 8 tons of cornmeal, 4 tons of bean cake, 2 tons of flour, 2.4 tons of gynostemma whole grass powder, 1 ton of wheat bran, 2 tons of corn stalks, 0.6 tons of imported fish meal, 1 ton of salt, and 1.4 kg of iron lactate , 2 kg of zinc gluconate, and 0.170 kg of magnesium gluconate were mixed evenly to obtain;
[0115] (2) Aerobic fermentation: put the fermented raw materials of step (1) into the fermenter, sterilize at 60-82°C for 30min, and press the cultured CICC 31188 Candida utilis seed liquid by 10% of the material in the tank The amount is sprayed into the tank for fermentation through a sterile atomizer, and fermented for 12 hours, and then CICC 10063 and CICC 10089 Bacillus subtilis (after mixing in equal parts) are sprayed into the fermenter according to 8% of the material, and fermented for 8 hours; through the fermentati...
Embodiment 2
[0120] A kind of preparation method of green organic selenium-rich feed, the method of this embodiment is the same as embodiment 1, and its difference is only that fermentation raw material is different, and the fermentation raw material of present embodiment is: 1.7 tons of cornmeal, 1.5 tons of bean cake, 3.36 tons of flour, 1.5 tons Ton of Gynostemma whole grass powder, 0.7 ton of wheat bran, 0.3 ton of fish meal, 0.5 ton of salt, 0.7kg of iron lactate, 1kg of zinc gluconate, 0.085kg of magnesium gluconate.
[0121] The feed of this embodiment is suitable for consumption by weaned pigs.
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