Primary tumor cell culture composition and use thereof
A primary tumor cell and composition technology, which is applied in the field of cell culture compositions, can solve the problems of slow growth and proliferation of primary tumor cells, and achieve the goals of increasing the growth and proliferation speed of primary tumor cells, reducing culture time, and improving efficiency Effect
Active Publication Date: 2016-10-12
海南海医药物安全性评价研究有限责任公司
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Problems solved by technology
[0005] In view of this, the present invention provides a primary tumor cell culture composition and its application, which solves the technical problem of slow growth and proliferation of primary tumor cells in the prior art
Method used
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Embodiment 1
[0015] A primary tumor cell culture composition, on the basis of 2L RPMI 1640 medium containing 10% fetal bovine serum, add 100mg mixture, the mixture consists of the following components by weight percentage:
[0016] Adiponectin 10g, TGF-β 1 40g,
[0017] IGF-1 20g, LIF 30g.
Embodiment 2
[0019] A primary tumor cell culture composition, on the basis of 2L RPMI 1640 medium containing 10% fetal bovine serum, add 100mg mixture, the mixture consists of the following components by weight percentage:
[0020] Adiponectin 5g, TGF-beta 1 50g,
[0021] IGF-1 10g, LIF 35g.
Embodiment 3
[0023] A primary tumor cell culture composition, on the basis of 2L RPMI 1640 medium containing 10% fetal bovine serum, add 100mg mixture, the mixture consists of the following components by weight percentage:
[0024] Adiponectin 15g, TGF-β 1 30g,
[0025] IGF-1 30g, LIF 25g.
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The invention discloses a primary tumor cell culture composition and a use thereof. In a RPMI 1640 culture medium containing 10% fetal calf serum, the primary tumor cell culture composition comprises, by weight, 5-15% of adiponectin, 30-50% of TGF-beta1, 10-30% of IGF-1 and 20-40% of LIF. By using the medium provided by the invention, a growth rate of the primary tumor cells extracted and isolated from the cancer patients is increased and tumor cell culture time is obviously reduced.
Description
technical field [0001] The invention relates to a cell culture composition, in particular to a primary tumor cell culture composition and its application. Background technique [0002] Tumor is a new organism formed by the body under the action of various tumorigenic factors, and the cells of local tissues lose their normal regulation of their growth at the gene level, resulting in abnormal proliferation and differentiation. Once a new organism is formed, it does not stop growing due to the elimination of the cause. Its growth is not regulated by normal body physiology, but destroys normal tissues and organs. This is especially obvious in malignant tumors (cancer). Compared with benign tumors, malignant tumors grow faster and show invasive growth, are prone to bleeding, necrosis, ulcers, etc., and often have distant metastasis, resulting in emaciation, weakness, anemia, loss of appetite, fever, and severe organ damage. Impairment of function, etc., eventually lead to death ...
Claims
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IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2501/105C12N2501/15C12N2501/235C12N2501/30
Inventor 姚茂忠董润璁田树红刘莹黄绵庆邢桂兰符健张开山王秀娟林杨钟克焱
Owner 海南海医药物安全性评价研究有限责任公司