Method for producing glycoside compounds by transforming microorganisms
A microbial transformation and glycoside technology, applied in the field of microbial transformation, can solve problems such as poor stability, low solubility, and limited promotion and use
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Embodiment 1
[0031] (1) Plate culture: take 20g of peeled potatoes, add 200ml of deionized water to boil and keep for 20min; Dilute the volume to 100ml with ionic water; sterilize at 115°C for 30 minutes, after the culture medium is cooled to about 50°C, pour the plate according to the aseptic method (about 15ml per dish), place it flat, and make a PDA plate after coagulation; Use an inoculation loop to pick the mycelium of C. brevipunctatus and streak it on a PDA plate, culture it in a constant temperature incubator at 28°C for 5 days, and store it at 4°C;
[0032] (2) Seed culture: Take 200g of peeled potatoes, add 1000ml of deionized water to boil and keep for 20min; filter after cooling, take the filtered clear liquid and add 20g of glucose, after the glucose is completely dissolved, add deionized water to make up to 1000ml, Divide into 250ml Erlenmeyer flasks, sterilize at 115°C for 30 minutes to obtain seed medium; pick a ring of mycelium from the plate of C. In a 250ml Erlenmeyer f...
Embodiment 2
[0037] (1) Plate culture: take 20g of peeled potatoes, add 200ml of deionized water to boil and keep for 20min; Dilute the volume to 100ml with ionic water; sterilize at 115°C for 30 minutes, after the culture medium is cooled to about 50°C, pour the plate according to the aseptic method (about 15ml per dish), place it flat, and make a PDA plate after coagulation; Use an inoculation loop to pick the mycelium of C. brevipunctatus and streak it on a PDA plate, culture it in a constant temperature incubator at 28°C for 5 days, and store it at 4°C;
[0038] (2) Seed culture: Take 200g of peeled potatoes, add 1000ml of deionized water to boil and keep for 20min; filter after cooling, take the filtered clear liquid and add 20g of glucose, after the glucose is completely dissolved, add deionized water to make up to 1000ml, Divide into 250ml Erlenmeyer flasks, sterilize at 115°C for 30 minutes to obtain seed medium; pick a ring of mycelium from the plate of C. In a 250ml Erlenmeyer f...
Embodiment 3
[0043] (1) Plate culture: take 20g of peeled potatoes, add 200ml of deionized water to boil and keep for 20min; Dilute the volume to 100ml with ionic water; sterilize at 115°C for 30 minutes, after the culture medium is cooled to about 50°C, pour the plate according to the aseptic method (about 15ml per dish), place it flat, and make a PDA plate after coagulation; Use an inoculation loop to pick the mycelium of C. brevipunctatus and streak it on a PDA plate, culture it in a constant temperature incubator at 28°C for 5 days, and store it at 4°C;
[0044] (2) Seed culture: Take 200g of peeled potatoes, add 1000ml of deionized water to boil and keep for 20min; filter after cooling, take the filtered clear liquid and add 20g of glucose, after the glucose is completely dissolved, add deionized water to make up to 1000ml, Divide into 250ml Erlenmeyer flasks, sterilize at 115°C for 30 minutes to obtain seed medium; pick a ring of mycelium from the plate of C. In a 250ml Erlenmeyer f...
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