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A kind of preparation method and application of blueberry anthocyanin chitosan nanoemulsion

A technology of blueberry anthocyanins and glycoside-chitosan, which is applied in the field of blueberry anthocyanin-chitosan nanoemulsions and its preparation, can solve undiscovered problems, prevent myopia from deepening, maintain dynamic stability, and protect microvessels Effect

Active Publication Date: 2019-02-05
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the current research on stabilizing anthocyanins is mainly focused on the auxiliary coloring effect and microencapsulation technology, and the method of improving the stability of anthocyanins and its preparation through nanoemulsions has not been found so far.

Method used

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  • A kind of preparation method and application of blueberry anthocyanin chitosan nanoemulsion
  • A kind of preparation method and application of blueberry anthocyanin chitosan nanoemulsion
  • A kind of preparation method and application of blueberry anthocyanin chitosan nanoemulsion

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Embodiment 1

[0028] This embodiment provides a kind of blueberry anthocyanin chitosan nanoemulsion, described blueberry anthocyanin chitosan nanoemulsion is prepared from blueberry fruit, chitosan hydrochloride and carboxymethyl chitosan, its preparation Methods as below:

[0029] The preparation process of blueberry anthocyanin chitosan nanoemulsion is as follows:

[0030] The first step: Ultrasonic extraction of blueberry pomace: the pomace after extracting the blueberry fruit juice is added according to the solid-liquid ratio of 1:20g / mL, and the pH=3.0±0.1, 60% (v / v) ethanol aqueous solution is added, and the ultrasonic power 300W, extraction temperature 60°C, ultrasonic extraction for 30 minutes to obtain blueberry anthocyanin extract;

[0031] The second step: freeze-drying: freeze-drying the blueberry anthocyanin extract to obtain the blueberry anthocyanin crude extract;

[0032] Step 3: Add 8 mg of blueberry anthocyanin crude extract to 30 mL of chitosan hydrochloride solution wi...

Embodiment 2

[0035] This embodiment provides a kind of blueberry anthocyanin chitosan nanoemulsion, described blueberry anthocyanin chitosan nanoemulsion is prepared from blueberry fruit, chitosan hydrochloride and carboxymethyl chitosan, its preparation Methods as below:

[0036] The preparation process of blueberry anthocyanin chitosan nanoemulsion is as follows:

[0037] The first step: Ultrasonic extraction of blueberry pomace: the pomace after extracting the blueberry fruit juice is added according to the solid-liquid ratio of 1:25g / mL, and the pH=3.0±0.1, 65% (v / v) ethanol aqueous solution is added, and the ultrasonic power 150W, extraction temperature 70°C, ultrasonic extraction for 15 minutes to obtain blueberry anthocyanin extract;

[0038] The second step: freeze-drying: freeze-drying the blueberry anthocyanin extract to obtain the blueberry anthocyanin crude extract;

[0039] Step 3: add 10 mg of blueberry anthocyanin crude extract to 30 mL of chitosan hydrochloride solution w...

Embodiment 3

[0042] This embodiment provides a kind of blueberry anthocyanin chitosan nanoemulsion, described blueberry anthocyanin chitosan nanoemulsion is prepared from blueberry fruit, chitosan hydrochloride and carboxymethyl chitosan, its preparation Methods as below:

[0043] The preparation process of blueberry anthocyanin chitosan nanoemulsion is as follows:

[0044] The first step: Ultrasonic extraction of blueberry pomace: the pomace after extracting the juice from the blueberry fruit is added according to the solid-liquid ratio of 1:15g / mL, and the pH=3.0±0.1, 62% (v / v) ethanol aqueous solution is added, and the ultrasonic power Under the conditions of 600W and extraction temperature of 50°C, ultrasonically extract for 40 minutes to obtain blueberry anthocyanin extract;

[0045] The second step: freeze-drying: freeze-drying the blueberry anthocyanin extract to obtain the blueberry anthocyanin crude extract;

[0046] Step 3: add 5 mg of blueberry anthocyanin crude extract to 30 ...

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Abstract

The invention provides a preparation method and application of a blueberry anthocyanin chitosan nanoemulsion, and belongs to the field of nanotechnology. The preparation method comprises the following steps: ultrasonically extracting blueberry pomace to obtain a blueberry anthocyanin extracting solvent; freeze-drying; adding a blueberry anthocyanin crude extract to a chitosan hydrochloride solvent with a concentration of 0.5-0.8mg / mL to obtain a mixed solvent I; dropwise adding a carboxymethyl chitosan solvent with the pH of 6.0 plus or minus 0.1 and a concentration of 0.5-2.0mg / mL to the mixed solvent I to obtain a blueberry anthocyanin chitosan nanoemulsion. The blueberry anthocyanin chitosan nanoemulsion prepared by the method can be used for preparing an eye drop and a blueberry anthocyanin beverage. The preparation method provided by the invention is simple, and the average particle size of the prepared blueberry anthocyanin chitosan nanoemulsion is less than 500nm; meanwhile, the loss rate of anthocyanin in the blueberry anthocyanin chitosan nanoemulsion prepared by the invention is extremely small as the storage time prolongs, and the prepared eye drop and beverage have the characteristics of high anthocyanin content, stable property and the like.

Description

technical field [0001] The invention relates to a nanoemulsion and its application, in particular to the blueberry anthocyanin chitosan nanoemulsion, its preparation method and its application in eye drops and beverages. Background technique [0002] Since nanotechnology was proposed by the scientific community in the 1980s, it is rising rapidly in today's technological development, and has become one of the world's cutting-edge technologies with unprecedented development in all countries in the world today. Nanotechnology is defined as a substance whose particle size is uniformly distributed between 1-500 nm. This research body has a new functional technology that has different characteristics from macroscopic substances and single isolated atoms within this scale. Nanotechnology covers a wide range of fields, including nanomaterials, nanobiology, and nanomicroscopy. The more refined technical finished products to meet higher-level requirements. At present, nanotechnology...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/107A61K36/45A61K47/36A61P27/02A61P27/10A23L2/02A23L2/60A23L2/68A23L33/00
CPCA23L2/02A23L2/60A23L2/68A23V2002/00A61K9/0048A61K9/1075A61K36/45A61K47/36A61K2236/333A23V2200/30
Inventor 高学玲葛姣岳鹏翔贺博
Owner ANHUI AGRICULTURAL UNIVERSITY