Method for improving salt resistance of rice
A technology of rice and ability, applied in the direction of chemical instruments and methods, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems of low success rate and long time-consuming, achieve high success rate, short time-consuming, improve yield effect
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Embodiment 1
[0010] Example 1: Obtaining of overexpressed OsKEA1 transgenic rice
[0011] The 3465bp full-length cDNA sequence fragment encoding the OsKEA1 gene was inserted into the binary vector pCAMBIA1300 for transformation of rice with a Ubiquitin promoter through Spel and PacI, and the p1300:ProUbiquitin:OsKEA1 vector was constructed, and the Agrobacterium-mediated The transgenic plants were transformed into the callus of wild-type rice 9522 (WT) by the genetic transformation method guided by the above method, and the overexpression transgenic plants were obtained. After the RNA was extracted from the transgenic plants and reverse-transcribed, ACTIN was used as an internal reference, and the specific primers of OsKEA1 gene were used as RT- PCR.
[0012] The results proved that the expression level of OsKEA1 gene in the transgenic plants was much higher than that of the wild type. Further Western blot analysis proved that the OsKEA1 content of the overexpressed OsKEA1 plants was signi...
Embodiment 2
[0013] Example 2: Salt tolerance of rice overexpressed OsKEA1 (Ov-OsKEA1)
[0014] Under the condition of hydroponic culture in the incubator (the light intensity is 100mol photons m-2 s-1), an additional 25mM NaCl was added to the normal medium (1 / 2 MS medium), and after 3 weeks of cultivation, the growth surface was observed. type.
[0015] The results showed that the growth of the overexpressed transgenic rice was significantly better than that of the wild type, which proved that the overexpression of OsKEA1 in rice can enhance the salt tolerance of rice, which provides a good basis for breeding salt-tolerant rice varieties.
Embodiment 3
[0016] Example 3: Effect of overexpression of OsKEA1 (Ov-OsKEA1) on rice field yield
[0017] Under field cultivation conditions, the seed yield of a single rice plant was compared and determined. The statistical results found that among the three transgenic lines, except for one overexpressed transgenic line whose yield per plant was slightly increased compared with the wild type, the other two overexpressed The yield per plant of the transgenic lines was about 20% higher than that of the wild type.
[0018] It is proved that overexpression of OsKEA1 in rice can increase the yield per plant of rice, which provides a good basis for breeding high-yielding rice varieties.
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