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Method for detecting alginic acid in fertilizer

A detection method and technology of alginic acid, which are applied in the direction of analysis by chemical reaction of materials, and material analysis by observing the influence on chemical indicators, can solve problems such as large influence and inability to detect, and achieve accurate measurement, Simple operation, accurate data effect

Inactive Publication Date: 2016-10-26
青岛海大生物集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the fertilizer contains PO 4 3- or NO 3 - Therefore, the application of the carbazole sulfuric acid method to the detection of seaweed fertilizers has great limitations; and the test found that the m-hydroxybiphenyl method is not affected by a large number of elemental nitrogen (including amino nitrogen, acyl nitrogen ), the interference of phosphorus and potassium, and the trace element Ca 2+ , Mg 2+ 、Cu 2+ , Zn 2+ , Mn 2+ , Fe 2+ , B, etc. are stable, and are stable to biochemical fulvic acids, but NO 3 - Humate and humate have a greater impact on it, when NO is added to the fertilizer containing alginate 3 - and humate, the detected alginic acid content compared with the recovery rate of pure alginic acid were: 10%-20%, 48.7%-64.6%

Method used

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  • Method for detecting alginic acid in fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] In sample 1, the theoretical content of alginic acid is about 1.4%, KNO 3 The content is 10%, and the detection steps are as follows:

[0041] (1) Drawing of standard curve

[0042] Prepare 0.1 g / L sodium alginate standard solution; weigh 0.01 g of sodium alginate, dilute to 100 mL, take 0, 0.1, 0.2, 0.3, 0.4, 0.5 mL respectively in test tubes, add distilled water to 0.5 mL. Add 3.0 mL of sodium tetraborate-sulfuric acid test solution after pre-cooling in an ice bath, shake to mix, put in a boiling water bath for 5 min, cool to room temperature in an ice bath, add 50 µL of m-hydroxybiphenyl with a micropipette, shake well and add Absorption was measured at 520 nm.

[0043] Take the content of sodium alginate as the abscissa and the corresponding absorbance value as the ordinate to draw the working curve.

[0044] (2) Determination of samples

[0045]After diluting the test solution by a certain number of times, accurately pipette 0.5 mL into a stoppered test tube ac...

Embodiment 2

[0057] In sample 2, the theoretical content of alginic acid is about 0.66%, and the content of humate is 8%. The detection steps are as follows:

[0058] (1) Drawing of standard curve

[0059] Prepare 0.1 g / L sodium alginate standard solution; weigh 0.01 g of sodium alginate, and dilute to 100 mL,

[0060] Take 0, 0.1, 0.2, 0.3, 0.4, 0.5 mL respectively in test tubes, add distilled water to 0.5 mL. Add 3.0 mL of sodium tetraborate-sulfuric acid test solution after pre-cooling in an ice bath, shake to mix, put in a boiling water bath for 5 min, cool to room temperature in an ice bath, add 50 µL of m-hydroxybiphenyl with a micropipette, shake well and add Absorption was measured at 520 nm.

[0061] Take the content of sodium alginate as the abscissa and the corresponding absorbance value as the ordinate to draw the working curve.

[0062] (2) Determination of samples

[0063] Since there is color in the sample, add the same volume of test solution as in color development to ...

Embodiment 3

[0072] Granular fertilizers include compound fertilizers, organic-inorganic compound fertilizers, etc.; take seaweed organic-inorganic compound fertilizers as an example.

[0073] The detection steps are as follows:

[0074] (1) Drawing of standard curve

[0075] Prepare 0.1 g / L sodium alginate standard solution; weigh 0.01 g of sodium alginate, dilute to 100 mL, take 0, 0.1, 0.2, 0.3, 0.4, 0.5 mL respectively in test tubes, add distilled water to 0.5 mL. Add 3.0 mL of sodium tetraborate-sulfuric acid test solution after pre-cooling in an ice bath, shake to mix, put in a boiling water bath for 5 min, cool to room temperature in an ice bath, add 50 µL of m-hydroxybiphenyl with a micropipette, shake well and add Absorption was measured at 520 nm.

[0076] Take the content of sodium alginate as the abscissa and the corresponding absorbance value as the ordinate to draw the working curve.

[0077] (2) Determination of samples

[0078] Take seaweed organic-inorganic compound fe...

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Abstract

The invention provides a method for detecting alginic acid in a fertilizer. The detection method is characterized by comprising the following steps: (1) taking alginic acid contents as the horizontal coordinate and corresponding light absorption values as the vertical coordinate to draw a work curve; (2) measuring a sample: adding a certain amount of sample into a colorimetric tube, and measuring the sample according to the method mentioned in the step (1). According to the measured light absorption value, a corresponding alginic acid content can be found in the work curve. The method has the advantages that the interference of macro elements such as nitrogen including amino nitrogen and acyl nitrogen, phosphor (PO4<3->), potassium, and the like is eliminated; the medium-micro elements (Ca<2+>, Mg<2+>, Cu<2+>, Zn<2+>, Mn<2+>, Fe<2+>, B, etc.) and biological fulvic acid are stable in the method; the interference of nitrate ions and color of humate is eliminated effectively, the operation is simple, the data is accurate, and the alginic acid content of a water soluble fertilizer can be precisely measured by the provided method.

Description

Technical field [0001] The invention belongs to the technical field of fertilizer detection, specifically a method for detecting alginic acid in fertilizers. technical background [0002] Alginic acid is a polyuronic acid extracted from brown algae. It is the main component of the cell wall and intercellular substance of brown algae. The alginic acid molecule is mainly composed of β-D-1,4-mannuronic acid (β-D -mannuronic acid, M) and α-L-1,4-guluronic acid (α-L-guLuronic, G) are two monomers composed of block linear polymers. There are three types of embedded monomers in the molecule. Section: Homopolymannuronic acid (M) n , Homopolyguluronic acid (G) n Alternate with M and G monomers (MG) n of blocks. There are obvious differences in the ratio of M and G in different algae. The M / G of alginic acid produced from kelp in my country is about 2.26, while the M / G of Sargassum is between 0.8 and 1.5. And the M / G of alginate in different parts is also different. In kelp, M / G ...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 单俊伟王海华王鹏郝晓晨张高丽
Owner 青岛海大生物集团股份有限公司