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Composite material-modified electrode used for measuring tyrosine concentration and application thereof

A technology for modifying electrodes and composite materials, which is applied in the field of composite materials modifying electrodes, can solve the problems of cumbersome steps and high detection costs of spectral or chromatographic methods, and achieve the effects of sensitive and efficient measurement, improved tyrosine response current signal, and excellent catalytic performance

Inactive Publication Date: 2016-10-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, spectroscopic or chromatographic methods have high detection costs and cumbersome steps, while electrochemical methods are favored by people because of their advantages such as easy operation, good selectivity, and low sensitivity.

Method used

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  • Composite material-modified electrode used for measuring tyrosine concentration and application thereof
  • Composite material-modified electrode used for measuring tyrosine concentration and application thereof
  • Composite material-modified electrode used for measuring tyrosine concentration and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation of a poly(p-aminobenzenesulfonic acid / gold nanocomposite modified electrode (PABSA / Au / GCE) for the determination of tyrosine concentration comprises the following steps:

[0023] (1) Glassy carbon electrode pretreatment: use α-Al with a particle size of 300nm and 50nm in sequence on the suede 2 o 3 Grind the glassy carbon electrode to the mirror surface with the paste polishing liquid, the mass volume ratio (mg / ml) of polishing powder and deionized water in the paste polishing liquid is 1:1-3, and then apply absolute ethanol and deionized water in sequence The electrode was ultrasonically washed for 1 min, and then the cleaned glassy carbon electrode was placed in a 0.5mol / L dilute sulfuric acid solution, and the cyclic voltammetry activation was performed at a sweep rate of 100mV / s in the potential range of -0.5 to 1.2V until the response was stable. Finally, rinse the glassy carbon electrode with deionized water and dry it for later use.

[0024] (2) Bef...

Embodiment 2

[0036] Preparation of a poly(p-aminobenzenesulfonic acid / gold nanocomposite modified electrode) (PABSA / Au / GCE) for tyrosine determination comprises the following steps:

[0037] (1) Glassy carbon electrode pretreatment: use α-Al with a particle size of 300nm and 50nm in sequence on the suede 2 o 3 Polish the glassy carbon electrode to the mirror surface with paste polishing liquid, then apply absolute ethanol and deionized water to ultrasonically wash the electrode for 90s, and then place the cleaned glassy carbon electrode in 0.6mol / L dilute sulfuric acid solution, in- The 0.6-1.3V potential range was activated by cyclic voltammetry at a scan rate of 120mV / s until the response was stable. Finally, the glassy carbon electrode was rinsed with deionized water and dried in the air for later use.

[0038] (2) The pretreated glassy carbon electrode was placed in 1.5mmol / L chloroauric acid (containing 0.05mol / L potassium nitrate) solution, and the constant potential deposition was ...

Embodiment 3

[0042] Preparation of a poly(p-aminobenzenesulfonic acid / gold nanocomposite modified electrode) (PABSA / Au / GCE) for tyrosine determination, comprising the following steps:

[0043] (1) Glassy carbon electrode pretreatment: use α-Al with a particle size of 300nm and 50nm in sequence on the suede 2 o 3 Polish the glassy carbon electrode to the mirror surface with the paste polishing solution, then use absolute ethanol and deionized water to ultrasonically wash the electrode for 2 minutes, and then place the cleaned glassy carbon electrode in 0.8mol / L dilute sulfuric acid solution, in- The 0.6-1.5V potential range was activated by cyclic voltammetry at a scan rate of 140mV / s until the response was stable. Finally, the glassy carbon electrode was rinsed with deionized water and dried in the air for later use.

[0044] (2) The pretreated glassy carbon electrode was placed in 2mmol / L chloroauric acid (containing 0.15mol / L potassium nitrate) solution, and the constant potential depos...

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Abstract

The invention discloses a composite material-modified electrode used for measuring tyrosine concentration and application thereof. According to the invention, a metal nanometer modified electrode is prepared by using a current-time method at first; and then a polyaminobezenesolfonic acid film is deposited on the surface of the metal nanometer modified electrode by using cyclic voltammetry so as to prepare the polyaminobezenesolfonic acid / metal nanometer composite material-modified electrode which is applied to detection of tyrosine. Results show that the composite material-modified electrode has excellent electrocatalytic activity to tyrosine and has the characteristics of good sensitivity, high accuracy, good stability, etc.

Description

technical field [0001] The invention belongs to the technical field of food analysis and detection, and in particular relates to a composite material modified electrode for tyrosine concentration measurement and its application. Background technique [0002] Tyrosine is an aromatic amino acid and one of the 20 amino acids used to synthesize proteins. It is a non-essential amino acid and widely exists in foods and beverages such as fruits, meat, wine and tea. It plays an important role in promoting the growth and development of the human body. Therefore, it is of great significance to establish a method for fast, convenient, accurate and sensitive detection of tyrosine content. [0003] At present, the determination methods of tyrosine mainly include high performance liquid chromatography, fluorescence spectroscopy, spectrophotometry, flow injection chemiluminescence, capillary electrophoresis and electrochemical methods. Among them, spectroscopic or chromatographic methods ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/30G01N27/3278
Inventor 韦真博张伟林王俊王永维
Owner ZHEJIANG UNIV
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