Building method and application of brucellosis tube agglutination test quality control chart

A technology for brucellosis and establishing methods, applied in measurement devices, disease diagnosis, biological testing, etc., can solve problems such as unseen, and achieve the effect of improving accuracy and control.

Inactive Publication Date: 2016-10-26
BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECH CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Quality control charts are mostly used in the field of analytical chemistry detection and health quarantine, but in the fie

Method used

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  • Building method and application of brucellosis tube agglutination test quality control chart
  • Building method and application of brucellosis tube agglutination test quality control chart
  • Building method and application of brucellosis tube agglutination test quality control chart

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: the establishment of Brucella quality control figure

[0038] 1. Experimental method

[0039] 1) Refer to the configuration of the turbidimetric tube for judgment

[0040] The reference turbidimetric tube for judgment is configured according to the requirements of Appendix E of "GB / T18646-2002 Animal Brucellosis Diagnostic Technique".

[0041] 2) Reagent compliance test

[0042] The purchased standard positive serum indicated ++ when it was diluted at 1:2400. Dissolve the lyophilized powder of the standard positive serum in 1 mL distilled water for reconstitution. Add 1mL of standard serum (4000IU / mL) into 36.5mL of carbolic saline, dilute to a solution of 1:37.5, and then serially dilute to 1:75, 1:150, 1:300, 1:600, 1: 1200, 1:2400, 1:4800, 1:9600, 1:19200 solutions. Dilute the antigen into a 1:20 working solution according to the antigen instructions. Add the antigen working solution to the above gradient dilution solution. After adding an equal v...

Embodiment 2

[0065] Embodiment 2: Application of quality control chart

[0066] The application of the quality control chart is to follow the warning lines and control lines of the quality control chart established in Example 1, analyze and test the standard positive serum and the sample to be tested simultaneously, and agglutinate the standard positive serum test tube with a dilution of 1:2400. After the experiment, the OD value of the supernatant was brought in and drawn as a control chart for control. In 22 daily experiments from September 2015 to October 2015, the OD values ​​of the supernatants after the agglutination test of 22 standard positive serum test tubes with a dilution ratio of 1:2400 were collected, and a control chart was drawn for control. The results See figure 2 . Compare the standard negative and positive sera with the reference turbidimetric tube by naked eyes, and the standard positive sera all show ++ when diluted at 1:2400.

[0067] Such as figure 1 As shown, ...

Embodiment 3

[0068] Embodiment 3: Using the quality control chart to determine the influence of experimental conditions on experimental results

[0069] 1. Establish a quality control chart and collect data in the early stage.

[0070] Routine serum agglutination tests and result judgments were carried out in accordance with the requirements of "GB / T 18646-2002 Brucellosis Laboratory Techniques". After the tube agglutination test was incubated, the supernatant of the standard positive serum at a dilution of 1:2400 was added to the VITEK turbidity test tube, and the OD value was read and recorded using a VITEK matching turbidimeter.

[0071] The 29 sets of data collected in the previous period after removing outliers by using the Dixon test method were used to draw quality control charts in chronological order. The center line (CL) of these 29 sets of data Upper warning limit (UWL) Lower warning limit (LWL) Upper Control Limit (UCL) Lower Control Limit (LCL) Use Excel to make a q...

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Abstract

The invention discloses a building method and application of a brucellosis tube agglutination test quality control chart. The building method and application has the advantages that the method applies the quality control chart to a tube agglutination test in an exploration and innovation manner, an optical density value is used as the control value to obtain an object controlled by the test, and a new statistical manner and reference are provided for the process control in the field of animal quarantine; in addition, during the application process of the quality control chart, the quality control chart can be regulated and optimized at any time along with the accumulation of test data so as to increase the precision and control power of the quality control chart.

Description

technical field [0001] The present invention relates to the field of animal quarantine, more specifically, to the field of inspection of brucellosis. Background technique [0002] Brucella is a Gram-negative coccus. According to different infectivity to different animals, Brucella is divided into six species and 17 biotypes. Brucellosis (referred to as brucellosis) is a zoonotic disease caused by Brucella infection. Animals such as cattle, sheep, and pigs are most susceptible to infection, causing miscarriage, mastitis, infertility, and inflammation of various tissues (such as testis and joints). All kinds of people are susceptible to contact with infected animals or eating sick animals and their dairy products. Patients usually have symptoms of a severe cold. Orchitis and epididymitis are common in male patients, while female patients are often accompanied by oophoritis, endometritis, and breast swelling and pain. Brucellosis is prevalent all over the world and poses a ...

Claims

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/50G01N2800/26
Inventor 饶红谷强郭铮蕾韩玥徐姗范斐李伟
Owner BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU INSPECTION & QUARANTINE TECH CENT
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