Unlock instant, AI-driven research and patent intelligence for your innovation.

A spectral electrochemiluminescence immunoassay method based on cdznse ternary quantum dots

An immunoassay method and electrochemistry technology, applied in the field of ECL immunoassay, to achieve the effects of good selectivity, good monochromaticity and good repeatability

Active Publication Date: 2018-03-13
SHANDONG UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Since ECL basic and applied research usually adopts the concept of detecting the total intensity of radiation to improve the signal-to-noise ratio and the sensitivity of ECL analysis, traditional ECL research rarely involves relevant spectral information, and biochemical sensing research based on ECL spectral information is basically in a blank state.
[0004] At the same time, although ternary quantum dots have more excellent optical properties than binary quantum dots, the basic research on ECL based on ternary quantum dots is still in a blank state, and the related ECL biochemical analysis and application research are still under development.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A spectral electrochemiluminescence immunoassay method based on cdznse ternary quantum dots
  • A spectral electrochemiluminescence immunoassay method based on cdznse ternary quantum dots
  • A spectral electrochemiluminescence immunoassay method based on cdznse ternary quantum dots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] A kind of ECL spectral type immunodetection method based on CdZnSe quantum dots, comprises steps as follows:

[0061] (1) Preparation of CdZnSe quantum dots

[0062] At room temperature, 0.80mL 0.20mol / L CdCl 2 The solution was added to a 100mL round-bottomed three-necked flask, and diluted to 50mL with ultrapure water. Add 27.7mg Zn(CH 3 COO) 2 and 72.5 mg of HMP, after stirring for 5 min, 34.6 μL of MPA was added. Adjust the pH of the solution to 8.0 with 6mol / L NaOH, slowly add 1.60mL 20.0mM NaOH 2 SeO 3 solution, after the above solution was heated to reflux at 100°C for 10min, 3.67mL N 2 h 4 ·H 2 O. Take the solutions with different reflux times and store them in the dark at 4°C. where Cd 2+ :Zn:Se:HMP:MPA:N 2 h 4 ·H 2 The molar ratio of O is 1:1:0.2:0.50:2.5:400. The UV-Vis absorption spectra of CdZnSe quantum dots coated with mercaptopropionic acid and sodium hexametaphosphate are shown in figure 1 As shown, the fluorescence spec...

Embodiment 2

[0074] An ECL spectroscopic immunoassay strategy based on CdZnSe quantum dots, the steps are the same as in Example 1, except that the concentration of alpha-fetoprotein antigen is 50.0 pg / mL.

Embodiment 3

[0076] An ECL spectroscopic immunoassay strategy based on CdZnSe quantum dots, the steps are the same as in Example 1, except that the concentration of alpha-fetoprotein antigen is 10.0 pg / mL.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a spectral electrochemiluminescence immunodetection method based on CdZnSe ternary quantum dots. Mainly including (1) preparation of CdZnSe quantum dots coated with double stabilizer, (2) preparation of CdZnSe quantum dot-labeled secondary antibody (Ab2∣CdZnSe NCs), (3) preparation of ECL immunosensor with CdZnSe quantum dots as labels and (4) four steps of ECL spectral detection of the immunosensor. The detection sensitivity of the method of the invention is high, and the detection limit of the alpha-fetoprotein antigen reaches 10.0fg / mL. The electrochemiluminescent immunosensor constructed in the present invention is constructed based on the specific recognition and binding between the antigen and the antibody, has little interference from other proteins, and has good selectivity.

Description

technical field [0001] The invention relates to a spectral type electrochemiluminescence (electrogenerated chemiluminescence, ECL) immunological detection method, in particular to a spectral type ECL immunological detection method using CdZnSe ternary quantum dots as markers, belonging to the technical field of ECL immunological detection. Background technique [0002] Electrochemiluminescence is an analytical technique that has been widely used in the fields of biochemical analysis and clinical diagnosis. For example, the ECL kit based on the ruthenium bipyridine / n-tripropylamine system developed by Roche has been used in the clinical diagnosis of more than 80 items such as tumor markers and hormones. Studies have shown that quantum dots (NCs or QDs), such as CdTe, CdSe, etc., can generate ECL radiation, which provides an important material basis for the construction of new ECL biochemical analysis strategies. For example, Liang et al. developed a near-infrared ECL immunod...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N21/76G01N33/574G01N33/58
CPCG01N21/76G01N33/574G01N33/588G01N33/68G01N2800/7028
Inventor 邹桂征张新
Owner SHANDONG UNIV