Cymbidium-ensifolium tissue-culture rapid propagation method

A tissue culture and rapid technology, applied in the field of plant tissue culture, can solve problems such as hindering the large-scale production of Jianlan, and achieve the effects of improving induction rate, high seedling rate and good growth.

Inactive Publication Date: 2016-11-09
成都东山兰韵农业有限公司
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Problems solved by technology

Some developed countries in foreign countries adopt industrial seedling breeding technology and tissue culture rapid propagation technology, which can not only create huge economic benefits, but also cultivat...

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  • Cymbidium-ensifolium tissue-culture rapid propagation method
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  • Cymbidium-ensifolium tissue-culture rapid propagation method

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Embodiment Construction

[0016] The specific embodiments of the present invention will be further elaborated below.

[0017] A kind of Jianlan tissue culture rapid propagation method provided by the invention comprises the following steps:

[0018] (1) Plant pretreatment: the mature plant female parent with a leaf length of 10-12 cm is placed in a cultivation room for cultivation, the cultivation temperature is 20-22°C, the illumination time is 15h / d, and the illumination intensity is 4000-5000lx. The time is 50 to 60 days; after a period of pretreatment, better quality pedicel can be obtained, so that the subsequent tissue culture effect is better.

[0019] (2) Material selection: After Jianlan pretreatment, select Jianlan with strong growth and good growth without diseases and insect pests, cut off the unflowered or blooming pedicel in the plant, remove the opened flowers and unopened flower buds on the pedicel, and cut off the middle part of the pedicel The pedicel buds on the nodes are used as ex...

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Abstract

The invention relates to the technical field of plant tissue culturing, in particular to a cymbidium-ensifolium tissue-culture rapid propagation method. The cymbidium-ensifolium tissue-culture rapid propagation method includes the following steps that plant pretreating, material selecting, material sterilizing, induction culturing, cluster-bud subculture multiplication culturing, rooting and hardening culturing and transplanting. According to the cymbidium-ensifolium tissue-culture rapid propagation method, as explant and the sterilization method are adopted, the induction rate of the explant can be greatly increased, and meanwhile cultured seedlings are high in survival seedling rate and good in growth vigor; the commercialized production requirements are met in a short time with the biotechnology means, and the technical support is provided for cymbidium-ensifolium large-scale and industrialization culturing in enterprises.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a rapid propagation method of Jianlan tissue culture. Background technique [0002] Jianlan is a terrestrial plant that grows in sparse forests, bushes, valleys or grass at an altitude of 600-1800 meters. Produced in Anhui, Zhejiang, Jiangxi, Fujian, Taiwan, Hunan, Guangdong, Hainan, Guangxi, southwestern Sichuan, Guizhou and Yunnan in China. Widely distributed in Southeast Asia and South Asia, north to Japan. At present, most of the propagation methods used are branch propagation, and few seeds are formed, and the development of seeds is relatively difficult. Basically, it is relatively difficult to germinate under natural conditions. Therefore, the main means of efficient propagation of Jianlan is tissue culture. Some developed countries in foreign countries adopt industrial seedling breeding technology and tissue culture rapid propagation technology, which can n...

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 姚胜琼陈泽彬
Owner 成都东山兰韵农业有限公司
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