Application of arbidol hydrochloride in preparing medicine for preventing and treating anti-Middle East respiratory syndrome coronavirus

A technology of arbidol hydrochloride, coronavirus, applied in the field of medicine, can solve problems such as no vaccine and drug treatment

Inactive Publication Date: 2016-11-09
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT) +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Of particular concern is the fact that no vaccine or drug treatment is currently available for this disease

Method used

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  • Application of arbidol hydrochloride in preparing medicine for preventing and treating anti-Middle East respiratory syndrome coronavirus
  • Application of arbidol hydrochloride in preparing medicine for preventing and treating anti-Middle East respiratory syndrome coronavirus
  • Application of arbidol hydrochloride in preparing medicine for preventing and treating anti-Middle East respiratory syndrome coronavirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1. Principles of Drug Evaluation

[0023] Virus amplification

[0024] African green monkey kidney cells (VERO) were pressed 3X10 5 cells / well, inoculated into 96-well plates, placed at 37°C, 5% CO 2 Cultivate until the monolayer grows. Inoculate a 100-fold dilution of the Middle East respiratory syndrome coronavirus clinical isolate into a 96-well plate full of monolayer cells, and place at 37°C, 5% CO 2 Culture for two days (including the normal control group).

[0025] Two days later, the degree of lesion is as high as 75%, put it in a -80°C ultra-low temperature refrigerator, and freeze and thaw once repeatedly, collect the virus liquid amplified by the cells, centrifuge at 3000r / min for 30 minutes, remove the sediment, put it in a small tube and put it in a -80°C ultra-low temperature freezer Long-term preservation.

[0026] Middle East respiratory syndrome coronavirus median lethal titer (TCID) 50 ) determination

[0027] African green monkey kidney...

Embodiment 2

[0034] Embodiment 2. The experimental result of Arbidol hydrochloride antiviral model

[0035] Drug Toxicity Evaluation of Arbidol Hydrochloride

[0036] After Arbidol hydrochloride was dissolved in DMSO, it was completely dissolved and was colorless and clear. After adding the culture medium and filtering, a little white precipitated out, and the solution was evenly clarified by shaking. The median toxic concentration (TC) of arbidol hydrochloride in African green monkey kidney cells (VERO) 50 ) is (61.3±0.02)μM, and the maximum non-toxic concentration is 48.25μM.

[0037] Middle East respiratory syndrome coronavirus median lethal titer (TCID) 50 ) determination

[0038] By Reed-Muench methodology, the detection of amplified MERS-CoV was 10 5.25 TCID 50 / 0.1mL.

[0039] Efficacy Evaluation of Arbidol Hydrochloride Against Middle East Respiratory Syndrome Coronavirus

[0040] The drug efficacy of arbidol hydrochloride was evaluated by MDCK cytopathic inhibition method a...

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Abstract

The invention discloses application of a medicine which contains arbidol as a main component, and a pharmaceutically acceptable salt of the arbidol in preventing and treating anti-Middle East respiratory syndrome coronavirus. The invention discloses for the first time that arbidol hydrochloride has an anti-virus function on Middle East respiratory viruses, can prevent Middle East respiratory syndrome coronavirus from infecting host cells, and can be adopted to treat Middle East respiratory syndrome coronavirus infection.

Description

technical field [0001] The invention relates to the application of arbidol hydrochloride in anti-Middle East respiratory syndrome coronavirus infection, and belongs to the field of medical technology. Background technique [0002] Middle East Respiratory Syndrome (Middle East Respiratory Syndrome, MERS), also known as New Sars, was first discovered in London in 2012 and named Novel Coronavirus No. 1 [Lodon1 novel CoV 2012]. Its structure is similar to that of SARS virus, and its origin in the Middle East. On May 23, 2013, the World Health Organization named the new coronavirus infection "Middle East Respiratory Syndrome". Coronaviruses belong to the genus Coronavirus of the family Coronaviridae in systematic classification. Viruses of the genus Coronavirus are positive-strand single-stranded RNA viruses with an envelope (envelope), with a diameter of about 80-120nm, and their genetic material is the largest among all RNA viruses. They only infect humans, mice, pigs, cats, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4045A61P31/14
CPCA61K31/4045
Inventor 杨子峰胡文辉李润峰赵金存赵昕钟南山
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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