Method for accurately identifying release fishes in caught paralichthys olivaceus

A brown flounder and accurate technology is applied to the field of accurately identifying and releasing fish from recaptured brown flounder, which can solve the problems of unautomated and large-scale operations, high misinterpretation rate, and increased workload, so as to reduce blindness, Accurate detection efficiency and the effect of reducing workload

Inactive Publication Date: 2016-11-09
中国水产科学研究院北戴河中心实验站
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is limited to single-plex PCR that amplifies a single locus in a system and uses traditional polyacrylamide gel electrophoresis detection methods
The disadvantage of single-plex PCR is that if multiple pairs of microsatellite markers need to be amplified, the workload will increase
The disadvantages of the traditional polyacrylamide gel electrophoresis detection method are low resolution, high misinterpretation rate, and inability to automate large-scale operations, etc.

Method used

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  • Method for accurately identifying release fishes in caught paralichthys olivaceus
  • Method for accurately identifying release fishes in caught paralichthys olivaceus
  • Method for accurately identifying release fishes in caught paralichthys olivaceus

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Embodiment 1

[0028] A kind of method for accurately distinguishing the released fish in recaptured brown flounder, the specific steps are as follows:

[0029] 1 Experimental materials

[0030] There are 6 female parents of brown flounder, numbered 8522, 2512, 2528, 9204, 5278, 1019; Five families were constructed, of which the parents of families 1, 3, 4 and 5 were known, which were 8522×0228, 2512×6#, 2528×9048 and 2512×8#, and family 2 was the offspring of wild flounder, not from The above 14 broodstock. 20 fishes were randomly selected from each family, a total of 100 fishes were constructed to construct a simulated recapture population of brown-toothed flounder, and paternity identification of brown-toothed flounder was carried out. Flounder is a natural group.

[0031] 2 Experimental methods

[0032] 2.1 Morphological observation

[0033] After observation, the skin on the non-eye side of the 100 brown flounder did not have blackening phenomenon, and the released group could not ...

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Abstract

The invention provides a method for accurately identifying release fishes in caught paralichthys olivaceus, and belongs to the field of proliferation and releasing. The method comprises the steps that firstly, morphological distinguishing is conducted; secondly, PCR amplification is conducted on mitochondrial DNA of the paralichthys olivaceus, and the suspected release paralichthys olivaceus is further determined through times type analysis; finally, fluorescently-labeled microsatellite marker multiple PCR amplification is conducted in a targeted mode, and the release individuals are determined. According to the method, blindness of release individual identification is reduced, work load is reduced, and the identification efficiency is improved.

Description

technical field [0001] The invention belongs to the field of multiplication and release, and in particular relates to a method for accurately identifying released fish from recaptured flounder. Background technique [0002] Brown flounder (Paralichthys olivaceus) is an important fishery resource in the Yellow and Bohai Sea regions of my country. It has the characteristics of large body, tender and delicious meat, and is deeply loved by consumers. Due to the increasingly serious environmental pollution of coastal waters and the increasing fishing intensity, the natural resources of wild flounder continue to decrease. In order to restore the resources of brown flounder, since the 1990s, scientific researchers put a large number of artificially bred brown flounder seeds into the Yellow Sea and Bohai Sea. What is the effect of multiplication and release? This is a topic of general concern to governments and fishery workers. The evaluation of the effect of multiplication and r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q1/686C12Q2600/124C12Q2563/107C12Q2525/151C12Q2537/143C12Q2531/113C12Q2565/125
Inventor 王青林司飞于姗姗王桂兴张红涛任建功于清海孙朝徽任玉芹姜秀凤
Owner 中国水产科学研究院北戴河中心实验站
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