Modifier for filter membrane for filter removal of leucocytes and modification method of filter membrane

A modifier and white blood cell technology, which is applied in the field of white blood cell filtration, can solve problems such as dissolution, and achieve the effect of not being easily injured, good solubility, and promoting platelet adhesion

Inactive Publication Date: 2016-11-16
广州新克力生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, hydrophilic polymers are highly water-soluble, and there will be dissolution problems in blood

Method used

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  • Modifier for filter membrane for filter removal of leucocytes and modification method of filter membrane
  • Modifier for filter membrane for filter removal of leucocytes and modification method of filter membrane
  • Modifier for filter membrane for filter removal of leucocytes and modification method of filter membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation of acrylate copolymer

[0030] Group

Dosage of dimethyl azobisisobutyrate (%)

A

0.35

B

0.40

C

0.45

D

0.50

[0031] Preparation:

[0032] Take equal moles of methyl methacrylate monomer and dimethylaminoethyl methacrylate monomer and mix them, place them in the reactor, replace the air in the system with nitrogen, and add 0.35-0.5% azobisiso by its mass. Dimethyl butyrate, under the protection of nitrogen, 400W microwave irradiation for 4min, to make the temperature reach 70℃, and then react under 150W microwave irradiation for 10min to obtain.

[0033] The polymerization conversion rate of the acrylate copolymer prepared in groups A-D was measured, and the results are shown in the following table:

[0034] Group

Embodiment 2

[0036] Example 2 Preparation of filter membrane modifier

[0037] The filter membrane modifier of Example 2 of the present invention is composed of amidated modified silk fibroin, polyvinylpyrrolidone and acrylate copolymer in a mass ratio of 0.6:0.4:1.

[0038] Preparation:

[0039] (1) Preparation of amidated modified silk fibroin: Dissolve silk fibroin in water to make a solution with a concentration of 10mg / mL, add 1mol / L urea aqueous solution at a volume ratio of 1:5, mix well, and then use Adjust the pH value to 4.75 with dilute hydrochloric acid solution, then add 2-fold weight of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride with silk fibroin, and react for 0.5h at room temperature. During the reaction, add dilute hydrochloric acid solution appropriately to keep the pH of the reaction solution at 4.75. After the reaction, add acetic acid buffer with a pH of 4.75 to decompose excess 1-(3-dimethylaminopropyl)-3-ethyl Carbodiimide hydrochloride, then the reaction...

Embodiment 3

[0041] Example 3 Preparation of filter membrane modifier

[0042] The filter membrane modifier of Example 3 of the present invention is composed of amidated modified silk fibroin, polyvinylpyrrolidone and acrylate copolymer in a mass ratio of 0.4:0.2:1.

[0043] The preparation method is the same as in Example 2.

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Abstract

The invention relates to a modifier for a filter membrane for filter removal of leucocytes and a modification method of the filter membrane. The modifier for the filter membrane is composed of amidated modified silk fibroin, polyvinyl pyrrolidone and an acrylate copolymer with the mass ratio of (0.05-2):(0.2-1):1, wherein the acrylate copolymer is composed of a methyl methacrylate monomer and a dimethylaminoethyl methacrylate monomer. The modifier for the filter membrane can be dissolved in a polar solvent such as water to prepare a solution, and the filter membrane is subjected to coating treatment by an infiltration modification method; the filter membrane after being treated by the modifier for the filter membrane has relatively good wettability, stability and compatibility, has relatively high filter removal rate on both the leukocytes and platelets, and has relatively low modifier washout rate, and avoids soluble substance pollution.

Description

Technical field [0001] The invention belongs to the technical field of leukocyte filtration, and specifically relates to a filter membrane modifier for filtering leukocytes and a modification method thereof. Background technique [0002] Infusion of leukocyte-containing whole blood or blood components can cause non-hemolytic fever reactions, human leukocyte antigen alloimmunization, blood transfusion-related virus infection, and graft-versus-host disease after blood transfusion. Therefore, filter out blood preparations before transfusion The white blood cells are of great significance. White blood cell filter is an important method to filter out white blood cells. Its principle is to use the principle of mechanical blocking and adsorption to trap white blood cells, and let red blood cells with smooth surface and strong deformability pass through. At present, the requirement of the third generation white blood cell filter is that the filtration rate reaches 99.99%. [0003] In add...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/04C09D133/14C09D189/00C09D139/06C08F220/34C08F220/14B01D39/08A61M1/02C08L67/02
CPCA61M1/0259B01D39/083B01D2239/0414B01D2239/0492B01D2239/0618C08F220/34C08J7/0427C08J2367/02C08J2433/14C08J2439/06C08J2489/00C09D133/14C09D139/06C09D189/00C08F220/14C08L89/00C08L39/06C08L33/14
Inventor 王新平
Owner 广州新克力生物科技有限公司
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