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Method for preparing flue-cured tobacco standard sample natural substrate positive material containing zineb residue

A natural matrix and acquisition method technology, applied in the field of natural matrix physical standard sample preparation, can solve the problems of unseen research reports, different extraction and purification behaviors from real test samples, and incomplete combination of target and matrix, etc., to achieve The acquisition method is simple, the use is convenient, and the effect of ensuring consistency

Inactive Publication Date: 2016-11-16
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The matrix RM is prepared by the addition method. Although the preparation is simple, there are situations in which the binding between the target and the matrix is ​​not completely consistent with the real test sample, which may lead to problems such as extraction and purification behaviors that are different from the real test sample. It is commonly used in tobacco leaf production, and there is no research report on the natural matrix RM of tobacco leaves containing zinc zinc

Method used

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  • Method for preparing flue-cured tobacco standard sample natural substrate positive material containing zineb residue
  • Method for preparing flue-cured tobacco standard sample natural substrate positive material containing zineb residue
  • Method for preparing flue-cured tobacco standard sample natural substrate positive material containing zineb residue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] ——Acquisition of natural matrix positive materials of flue-cured tobacco physical standard samples containing zinc zinc residues

[0030] Experimental location: Huangguan Modern Tobacco Agriculture Professional Cooperative, Hongta District, Yuxi City, with an experimental area of ​​134 m 2 . Experimental Materials:

[0031] 1) Tobacco plants: The tobacco leaf variety is Yunyan 87, and the row spacing of the tobacco plants is 50cm*120cm. According to the normal management of the local tobacco leaf production technology, the tobacco plants grow well.

[0032] 2) Reagent: Reagent name 65% Zinc wettable powder, Sichuan Guoguang Agrochemical Co., Ltd.

[0033] 3) Instrument: GC-MS (PE clarus 680-600T).

[0034] experimental method:

[0035] 1) Application: application weather: sunny. Application method: uniform spraying method is used to spray Zinc wettable powder on the cultivated tobacco plants, and the application rate is 150g per mu. The pesticide preparation metho...

Embodiment 2

[0050] ——Study on the uniformity of the natural matrix positive material of the flue-cured tobacco physical standard sample containing zinc zinc residue

[0051] Experimental Materials:

[0052] 1) Sample: the sample prepared according to the method described in Example 1, and the sampling time was November 2013.

[0053] 2) Reagent: Same as Example 1.

[0054] 3) Instrument: Same as Example 1.

[0055] experimental method:

[0056] Put the roasted, crushed, sieved, and mixed tobacco leaf powder into brown sample bottles, 10g per bottle, and affix a numbered sample label. Sampling method: number the sample bottles sequentially, and randomly select 20 samples using a random number table. bottle, each bottle is detected 3 times, and the detection method is the same as in Example 1. The results were statistically analyzed for uniformity by the F test method, and the test data results and variance analysis results are shown in Table 1 and Table 2. The F value of Zinc residues...

Embodiment 3

[0060] ——Study on the stability of the natural matrix positive material of the flue-cured tobacco physical standard sample containing Zinc residues

[0061] Experimental Materials:

[0062] 1) Samples: samples prepared according to the method described in Example 1, and the sampling time was taken monthly from January 2014 to December 2015.

[0063] 2) Reagent: Same as Example 1.

[0064] 3) Instrument: Same as Example 1.

[0065] Experimental method: Put the tobacco leaf powder that has been roasted, crushed, sieved, and mixed into sample bottles, 10g per bottle, affix a sample label with a number, store it at -20°C, and sample it once a month. 24 months, since the measurements are carried out under (in-laboratory) reproducible conditions, which also includes measurement instability. The results of the long-term stability study are shown in Table 3 and Table 4. The results show that if the p value (0.414) is greater than 0.05, it means that the regression is not significa...

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Abstract

The invention discloses a method for obtaining natural matrix positive materials of flue-cured tobacco physical standard samples containing Zineb residue. First, spray concentration 65% Zinc wettable powder to the cultivated tobacco plants, and the application rate per mu is 150g; each tobacco plant gets the tobacco leaves of the 10th, 11th, and 12th leaf positions, 2 hours after the spraying, and On days 1, 3, 5, 7, 10, 14, 21, 28, 35, and 42, samples were taken to detect the residual content of zinc zinc, and the change curve was drawn; according to the change curve, samples were taken on the 42nd day after application, and the picked tobacco leaves were dried Roasting, crushing, sieving and mixing; the content of zinc zinc in the natural matrix positive material of the physical standard sample of flue-cured tobacco is 5.40±0.34mg / kg. The method can prepare a natural matrix standard sample for quality control of Zinc residue analysis in flue-cured tobacco that is consistent with the real test sample, which ensures the consistency of the extraction rate of the quality control sample and the test sample under the same conditions. The prepared natural matrix standard The sample is uniform, stable, easy to store and use.

Description

technical field [0001] The invention belongs to the technical field of preparation of natural matrix physical standard samples for quality control of pesticide residue detection, and in particular relates to a method for obtaining natural matrix positive materials of flue-cured tobacco physical standard samples containing zinc zinc residues. Background technique [0002] The matrix RM for pesticide detection is very lacking. In the detection of pesticide residues, it is necessary to establish pure product reference materials and matrix reference materials to meet the requirements of detection quality control, solving problems encountered in analysis, and ensuring accurate results. EU regulations on residue testing require the use of standard samples to control testing quality and verify testing methods. The matrix RM is prepared by the addition method. Although the preparation is simple, there are situations in which the binding between the target and the matrix is ​​not co...

Claims

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Application Information

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IPC IPC(8): G01N1/28
CPCG01N1/28G01N2001/2893
Inventor 师君丽孔光辉逄涛李勇
Owner YUNNAN ACAD OF TOBACCO AGRI SCI