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A control method for a microfluidic chip in which microscopic particles rotate around three coordinate axes

A technology of microfluidic chips and control methods, applied in fluid controllers, microstructure devices, chemical instruments and methods, etc., can solve the problem that the three rotational degrees of freedom of cells are difficult to achieve at the same designated position

Active Publication Date: 2018-11-09
HOHAI UNIV CHANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a microfluidic chip and its control method to address the problem that the three rotational degrees of freedom of cells are difficult to achieve at the same designated position in the micromanipulation of microscopic particles (cells)

Method used

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  • A control method for a microfluidic chip in which microscopic particles rotate around three coordinate axes
  • A control method for a microfluidic chip in which microscopic particles rotate around three coordinate axes
  • A control method for a microfluidic chip in which microscopic particles rotate around three coordinate axes

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Embodiment 1

[0032] Such as figure 1 As shown, a microfluidic chip of the present invention includes an upper indium tin oxide film 1, a microfluidic chamber 2, a photoconductive layer 3 and a lower indium tin oxide film 4 from top to bottom; wherein the upper and lower The indium tin oxide film is a transparent conductive film, and the upper and lower indium tin oxide films are suitable for respectively connecting the output terminal and the ground terminal of an alternating signal source; and the corresponding light patterns (miniature light patterns) pass through the The lower indium tin oxide thin film irradiates the lower end surface of the photoconductive layer to adjust the attitude and / or position of the cells in the microfluidic chamber.

[0033] The upper indium tin oxide thin film 1 can be used to observe the cell operation through the upper indium tin oxide thin film 1 .

[0034] The microfluidic chip is suitable for three-dimensional rotation of electrically neutral cells or ...

Embodiment 2

[0041] On the basis of Embodiment 1, Embodiment 2 provides a control method for a microfluidic chip.

[0042] The control method of the microfluidic chip is to adjust the posture and / or position of cells in the microfluidic cavity in the microfluidic chip through corresponding light patterns (miniature light patterns).

[0043] The microfluidic chip is suitable for using the microfluidic chip as described in Example 1.

[0044] The method for adjusting the attitude of the cells includes: defining the horizontal virtual axis of rotation of the cells on the horizontal plane, and controlling the cells to flip around the horizontal virtual axis; that is, projecting a rectangular light pattern according to the position of the cell, so that the rectangular light pattern is formed on the lower end surface of the photoconductive layer A light-dark boundary line (the projected area is the bright film area, and the non-projected area is the dark film area), the light-dark boundary line ...

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Abstract

The invention relates to a micro-fluidic chip with microscopic particles rotating around a three-coordinate axis and a control method of the micro-fluidic chip. The micro-fluidic chip comprises an upper indium tin oxide thin film, a micro-fluid cavity, a photoconductive layer and a lower indium tin oxide thin film in sequence from top to bottom, wherein the upper indium tin oxide thin film and the lower indium tin oxide thin film are respectively connected to the output end and the grounding end of an alternating signal source; corresponding light graphs are respectively irradiated to the lower end surface of the photoconductive layer through the lower indium tin oxide thin film to adjust the posture and / or position of a cell in the micro-fluid cavity. The micro-fluidic chip with the microscopic particles rotating around the three-coordinate axis is suitable for enabling the cell to rotate in a three-dimensional manner to adjust of the position and the posture of the cell, is easy to operate, and can avoid the producing of a complicated physical entity electrode array or other contact type control components on the chip.

Description

technical field [0001] The invention provides a method for three-dimensionally rotating microscopic particles, and relates to the field of microelectromechanical systems, in particular to the field of multi-degree-of-freedom rotation of cells by microscopic electric forces. Background technique [0002] Cell micromanipulation is a very important technology in modern bioengineering, which is widely used in biomedical engineering, life science, physics, chemistry and other fields. With the development of biotechnology, the current micromanipulation has entered the operation level of subcellular level. The regulation of cell position and attitude is an important part of cell micromanipulation. Therefore, it is of great significance to carry out research on the automatic regulation of cell position and attitude. In many micromanipulations, such as intracytoplasmic sperm microinjection, detection of embryonic polar body activity, oocyte nuclear transfer and cloning, it is necess...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00B81B3/00G01N27/447
CPCB01L3/502707B01L2200/027B01L2200/0647B01L2400/0424B81B3/0051B81B3/0062G01N27/447
Inventor 朱晓璐杨逸飞聂晟楠戴含晖陈宇恒李欣
Owner HOHAI UNIV CHANGZHOU