Kit for culturing mesenchymal stem cells and culturing method

A technology of mesenchymal stem cells and kits, applied in cell culture active agents, animal cells, culture process and other directions, can solve the problem that the quantity and quality of mesenchymal stem cells cannot meet the requirements of the test, the kits are few, and the time of laboratory researchers is wasted It can improve the cell proliferation rate, ensure the quality and activity, and save the cell passaging time.

Active Publication Date: 2016-12-07
BEIJING YULONG SHENGSHI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that there are few kits specially used for culturing mesenchymal stem cells in the prior art, and the methods used by experimenters to separate and cultivate mesenchymal stem cells, the various reagents used for cultivating are not the same, resulting in the mesenchymal stem cells being cultured differently. The quantity and quality of mesenchymal stem cells cannot meet the requirements of the experiment, wasting the time and funds of laboratory researchers, etc. The invention provides a kit for culturing mesenchymal stem cells

Method used

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  • Kit for culturing mesenchymal stem cells and culturing method
  • Kit for culturing mesenchymal stem cells and culturing method
  • Kit for culturing mesenchymal stem cells and culturing method

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0048] Such as figure 1 As shown, Example 1 of the present invention provides a kit for culturing mesenchymal stem cells, comprising a box body 1 and a box cover 2 hinged to the box body 1, and a compartment is arranged laterally in the box body 1. Plate 3, the box body 1 is divided into a reagent storage box 4 and a tool box 5 from top to bottom through the partition 3, the reagent storage box 4 is used to hold rice reagents, which is convenient for collecting umbilical cord tissue and cell culture, and the tool box 5 are used to store the tools required for culturing cells and collecting umbilical cords. The tool box 5 is provided with a pull-out box 6 for placing the tools needed for collecting or cultivating mesenchymal stem cells. The pull-out box 6 and the tools The box 5 is slidably connected, and the drawer box 6 is slidably connected with the tool box 5, which is convenient for taking out or storing the tools. The tools include spare test tubes, culture bottles or cul...

Embodiment 2

[0051] Such as Figure 2-5 As shown, Example 2 of the present invention further defines the structure of the kit on the basis of Example 1.

[0052] It should be noted that the reagent storage box 4 is provided with a storage basket 8, which can store the reagent bottle 7, which is convenient for cleaning, and at the same time, it is convenient to take out the reagent bottle 7 at the same time, and the transportation is more convenient. A baffle plate 9 is longitudinally arranged in the storage basket 8, and the accommodating basket 8 is divided into a storage bin 10 and a reagent bin 11 by the baffle plate 9, and the storage bin 10 is used to place test tubes or petri dishes in the test, and the reagent bin 11 is used to place the reagent bottle 7, and the reagent box is placed to collide during transportation, which improves the safety of transportation. The storage compartment 10 is provided with a liner 12, and the liner 12 has a through hole, and the test tube for holdin...

Embodiment 3

[0056] Example 3 of the present invention further defines the composition of the umbilical cord preservation solution in the kit on the basis of Example 1. The umbilical cord preservation solution mainly consists of gemifloxacin, transferrin, 2′,3′-dideoxyadenosine- 5-triphosphate, kanamycin and tobramycin are dissolved in α-MEM medium aqueous solution, and 10 mg of gemifloxacin, 5 mg of transferrin, 2′ , 1 mg of 3′-dideoxyadenosine-5-triphosphate, 50 U of kanamycin, and 45 U of tobramycin, and the concentration of the α-MEM medium aqueous solution is 8 mg / mL.

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Abstract

The invention provides a kit for culturing mesenchymal stem cells and a culturing method. The kit comprises a kit body and a kit cover hinged to the kit body; a partition plate is transversely arranged in the kit body, the kit body is internally divided into a reagent storage box and a tool box, the tool box is internally provided with a drawing box, the reagent storage box is internally provided with multiple reagent bottles which contain an umbilical cord preservation solution, a culture solution, additives, growth factors I, growth factors II, digestive juice, a cleaning solution and a culture-medium. The kit is simple in structure and is specially used for culturing the mesenchymal stem cells, the culturing efficiency of the mesenchymal stem cells is improved, more mesenchymal stem cells can be cultured in large quantities, demands of people can be met, and the economic cost is reduced; meanwhile, reagent formulas of the reagents in the kit are reasonable and effective, the nutritional requirements of mesenchymal stem cell growth can be met, value added growth of the mesenchymal stem cells is effectively promoted, and the cell survival rate is increased.

Description

technical field [0001] The invention belongs to the field of cell culture, in particular to a kit for culturing mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs) are a kind of stem cells with self-replication ability and multi-lineage differentiation potential, which can continuously self-renew and differentiate into many kinds of tissue cells. Stem cells are the origin cells of the body and the progenitor cells that form various tissues and organs of the human body. Under certain conditions, they can transform into one or more cells that constitute human tissues or organs. For example, mesenchymal stem cells have The capacity of bone cells, fat cells, chondrocytes, and other cell types. [0003] Mesenchymal stem cells have high differentiation potential and can differentiate in multiple directions. It can differentiate into tissues such as bone, cartilage, muscle, tendon, ligament, nerve, liver, endothelium, and heart muscle. Mesenchymal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/0775
CPCC12N5/0668C12N2500/30C12N2500/34C12N2500/76C12N2501/11C12N2501/115C12N2501/998
Inventor 张晓南吴芳春陈虎侍晓云张斌
Owner BEIJING YULONG SHENGSHI BIOTECH CO LTD
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