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Slide glass cover plate for slide glass sample treatment and use method thereof

A technique for specimen processing and slide loading, applied in the direction of laboratory containers, chemical instruments and methods, support of flat carriers, etc., can solve the problem of not being able to heat, increasing the complexity of the operation process and operating errors, containers or carriers Insufficient heat and other problems, to achieve the effect of improving space utilization, improving reliability and repeatability

Active Publication Date: 2016-12-14
XIAMEN TONGLING BIOMEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the containers or carriers commonly used for manual handling of slide specimens are not heat-resistant and do not have the function of being heated.
In the middle of the slide specimen processing, the slide specimen needs to be transferred to a heatable container, and then transferred to the operating table at room temperature, which increases the complexity of the operation process and operational errors

Method used

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  • Slide glass cover plate for slide glass sample treatment and use method thereof
  • Slide glass cover plate for slide glass sample treatment and use method thereof
  • Slide glass cover plate for slide glass sample treatment and use method thereof

Examples

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Effect test

Embodiment example

[0056] Example of implementation: the slide cover plate has the following dimensions: the length of the depth positioning surface 2 is 54mm, the distance between the inner sides of the two width positioning blocks 3 is 20mm, D1=0.2mm, D2=0.5mm, the length of the capillary plane is 40mm, The width is 20mm, and the angle between the groove opening surface and the depth positioning surface is 60 degrees. The slide has a length of 75mm and a width of 25mm. The thickness of the sliced ​​specimen is about 0.005mm. Experiments have shown that a capillary gap is formed between the capillary plane of the slide and the cover plate, which can form a capillary siphon effect, and the reagent fills the gap when adding a sample.

[0057] During the immunohistochemical staining process of the slide assembly 103 of the present invention, the liquid in the gap is replaced evenly. Two heating methods were tested: direct immersion of the slide assembly 103 in the boiling heat repair solution an...

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Abstract

The invention discloses a slide glass cover plate for slide glass sample treatment and a use method thereof. The slide glass cover plate comprises a capillary plane, a depth positioning plane, a width positioning block, a bottom positioning block, a slot lateral wall, a slot opening wall and a flow guide port. A capillary gap is formed in the middle of the slide glass component formed by the combination of the slide glass cover plate and the slide glass; in a sampling process, a reagent uniformly fills in the gap under the capillary siphoning effect; when the slide glass component is placed in any form (vertical or inclined), the reagent in the gap does not drain away; when a new reagent is added, the new reagent enters into the gap from the sampling slot and the original fluid in the gap flows out of the gap flow guide port at the bottom of the slide glass component and is automatically replaced; before the new reagent is added each time, the operation of clearing and drying the reagent remained in the last step is not required; the slide glass component is made of a heat-resisting material and can be soaked or locally inserted into a thermal medium for heating and preserving heat, so that the slide glass sample can run in a same device from beginning to end.

Description

technical field [0001] The invention belongs to the field of biological sample processing, in particular to a slide cover plate for biological slice specimen processing and a use method thereof. Background technique [0002] The whole process of protein, pathogen or gene detection on slide specimens (such as immunohistochemistry and hybrid gene detection, etc.) requires dozens of complicated steps, and various factors such as environment and human operation seriously affect the accuracy of test results. Simplifying the operation process and reducing factors affected by the environment are the top priorities for improving the efficiency and accuracy of specimen detection. [0003] At present, in the inspection process, slide specimens are laid out in tiles, occupying a large space, which limits the flow rate and efficiency of slide specimen processing. [0004] Reagent injection is sprayed from the top of the sheet, and the error of the injection position is large. Open inc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/31
CPCG01N1/28G01N1/31G02B21/34G01N1/312B01L9/52B01L2200/02B01L2300/045B01L2300/047B01L2300/0609B01L2300/0822B01L3/508
Inventor 郭金灿郭恩明
Owner XIAMEN TONGLING BIOMEDICAL TECH CO LTD
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