Virus-free seeding raising method of Beijing 553 sweet potatoes

A sweet potato and virus technology, applied in horticultural methods, botanical equipment and methods, seed immunity, etc., can solve the problems of quality degradation, yield reduction, infection, etc., and achieve the effect of preventing variety degradation, improving yield and quality, and realizing cultivation

Inactive Publication Date: 2016-12-21
TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, Beijing 553 sweet potatoes are easily infected by a variety of viruses and pathogenic bacteria, resulting in degradation of species, reduced yield, and quality degradation. Especially when planted in large areas, it is easy to cause large areas of sweet potatoes to be harmed by viruses and cause huge economic losses.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for seedling-free Beijing 553 sweet potato, characterized in that the steps are as follows:

[0019] (1) Select Beijing 553 sweet potato pieces of the same size and put them into a variable temperature water bath for virus passivation treatment. The method is: first raise the temperature of the water bath to 57°C, soak the sweet potato pieces for 3 minutes, and then lower the water temperature to 51°C, soak the sweet potato pieces for 30 minutes;

[0020] (2) Put the sweet potato pieces after warm water passivation virus treatment into 50% carbendazim disinfectant solution diluted 800 times for 15 minutes, put the processed sweet potato pieces into the container, cover with 10cm thick sterilized fine sand, placed in a plastic greenhouse at a temperature of 30°C for germination;

[0021] (3) when the seedling of sweet potato piece grows to 30cm, cut the vigorous 3cm stem tip of growth, cut off blade;

[0022] (4) Disinfect the stem tip that has been cut off th...

Embodiment 2

[0028] A method for seedling-free Beijing 553 sweet potato, characterized in that the steps are as follows:

[0029] (1) Select Beijing 553 sweet potato pieces of the same size and put them into a variable temperature water bath for virus passivation treatment. The method is: first raise the temperature of the water bath to 57°C, soak the sweet potato pieces for 3 minutes, and then lower the water temperature to 51°C, soak the sweet potato pieces for 30 minutes;

[0030] (2) Put the sweet potato pieces after warm water passivation virus treatment into 50% carbendazim disinfectant solution diluted 800 times for 15 minutes, put the processed sweet potato pieces into the container, cover with 10cm thick sterilized fine sand, placed in a plastic greenhouse at a temperature of 30°C for germination;

[0031] (3) when the seedling of sweet potato block grows to 40cm, cut the vigorous 5cm stem tip of growth, cut off blade;

[0032] (4) Disinfect the stem tip that has been cut off th...

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PUM

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Abstract

The invention discloses a virus-free seedling raising method of Beijing 553 sweet potatoes. The method comprises the following steps: firstly, sweet potato pieces are selected to undergo virus inactivation treatment and disinfection treatment, and then the sweet potato pieces are put into fine sand for accelerating germination; then, vigorous stem tips of 3cm long are cut when seedlings of the sweet potato pieces grow to be 30 cm long, leaves are cut off, the stem tips are disinfected, 0.2-0.3mm growth point with one leaf primordium is stripped off under anatomical lens and inoculated into an MS medium, and a culture dish is put into a tissue culture room for culture; and virus detections are carried out when the stem tips grow into plantlets with 4 leaves, and healthy and virus-free plants are left; and finally, stem sections of 4cm long are cut out and inserted into a tray filled with a substrate, and finally seedlings are cultivated in a greenhouse. According to the invention, virus-free seedling-raising of Beijing 553 sweet potatoes is realized, cultivation of virus-free sweet potato seedlings can be effectively achieved, variety degeneration is prevented, and yield and quality of sweet potatoes are enhanced.

Description

technical field [0001] The invention relates to the field of plant tissue culture rapid propagation and detoxification, in particular to a method for raising seedlings of Beijing 553 sweet potato detoxification. Background technique [0002] Sweet potato is one of the main food crops, and it is also an important feed crop. It has high economic value, so the planting area is more and more extensive. Among them, Beijing 553 sweet potato is one of the edible sweet potato varieties, and the tubers are spindle-shaped. Skin color orange. However, Beijing 553 sweet potatoes are easily infected by a variety of viruses and pathogenic bacteria, resulting in degeneration of species, yield reduction, and quality degradation. Especially when planted in large areas, it is easy to cause large areas of sweet potatoes to be harmed by viruses and cause huge economic losses. Plant shoot tip virus-free technology is to use the characteristics of plant growth points that do not contain or conta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01C1/08
CPCA01H4/008A01C1/08
Inventor 王立国郭子江张嘉李建军朱永伶芮淑会王鸿鸣李彦阳
Owner TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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