The effective site, preparation method and application of Cerulea broadensis for inhibiting hdac1 enzyme

An effective part, the technology of wide tendon vine, applied in the field of plant extracts, can solve the problems that have not been seen in research and application

Active Publication Date: 2019-12-10
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no research and application on the activity of vines inhibiting HDAC1 enzyme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiments 1 to 3 are specific implementations of the active part, preparation method and application of the vine of the present invention for inhibiting HDAC1 enzyme, wherein embodiment 1 is the best embodiment.

[0020] Example 1

[0021] The preparation method comprises the following steps: crushing vine vine, using an ethanol solution with a mass percentage concentration of 75% to 85% for ultrasonic extraction for 1 hour, and then filtering to obtain an ultrasonic extraction solution and a filter residue, and adding a mass percentage concentration of 75% to 85% to the filter residue. % ethanol solution reflux extraction 3 times, each time for 0.5 hours, combined ultrasonic extraction solution and reflux extraction extract, concentrated and dried to obtain extract; add distilled water to disperse the extract to obtain extract dispersion, and then use petroleum ether, acetic acid Ethyl ester and n-butanol are used as solvents to extract the extract dispersion, and af...

Embodiment 2

[0023] The preparation method comprises the following steps: crushing vine vine, ultrasonically extracting with an ethanol solution with a mass percentage concentration of 85% to 95% for 3 hours, and then filtering to obtain an ultrasonic extraction solution and a filter residue, and adding a mass percentage concentration of 85% to 95% to the filter residue. % ethanol solution reflux extraction 3 times, 1 hour each time, combined ultrasonic extraction solution and reflux extraction extraction solution, concentrated and dried to obtain extract; add distilled water to disperse the extract to obtain extract dispersion, and then use petroleum ether, ethyl acetate Ester and n-butanol are used as a solvent to extract the extract dispersion, and after the solvent is evaporated, the petroleum ether part, the ethyl acetate part and the n-butanol part of the vine are obtained; the remaining extract dispersion after extraction is concentrated, Dry to obtain the aqueous phase extraction pa...

Embodiment 3

[0025] The preparation method comprises the following steps: pulverize the rattan vine, use an ethanol solution with a mass percentage concentration of 65% to 75% for ultrasonic extraction for 0.5 hours, and then filter to obtain an ultrasonic extraction solution and a filter residue, and the filter residue is added with a mass percentage concentration of 65% to 75% % ethanol solution reflux extraction 2 times, each 2 hours, combined ultrasonic extraction and reflux extraction extract, concentrated and dried to obtain extract; add distilled water to disperse the extract to obtain extract dispersion, and then use petroleum ether, ethyl acetate Ester and n-butanol are used as a solvent to extract the extract dispersion, and after the solvent is evaporated, the petroleum ether part, the ethyl acetate part and the n-butanol part of the vine are obtained; the remaining extract dispersion after extraction is concentrated, Dry to obtain the aqueous phase extraction part of the broad t...

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PUM

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Abstract

The invention discloses a tinospora sinensis HDAC1 enzyme inhibition effective part, as well as a preparation method and application thereof, and belongs to the technical field of plant extracts. The corydalis pygmaea effective part is one of a corydalis pygmaea petroleum ether part and a corydalis pygmaea aqueous extraction part, or a mixture of more than two of a corydalis pygmaea petroleum ether part, a corydalis pygmaea ethyl acetate part, a corydalis pygmaea n-butyl alcohol part and a corydalis pygmaea aqueous extraction part in an arbitrary mass ratio. The preparation method comprises the following steps of: grinding corydalis pygmaea, refluxing and extracting with an ethanol solution having a volume percentage concentration of 65-95 percent, concentrating and drying the extract to obtain an extract; dispersing the extract with distilled water to obtain an extract dispersant, extracting the extract dispersant sequentially with petroleum ether, ethyl acetate and n-butyl alcohol, and volatilizing a solvent to obtain a corydalis pygmaea petroleum ether part, a corydalis pygmaea ethyl acetate part and a corydalis pygmaea n-butyl alcohol part; and concentrating and drying the extracted extract dispersant to obtain a corydalis pygmaea water-phase extraction part. The corydalis pygmaea HDAC1 enzyme inhibition effective part can be applied to preparation of medicines for inhibiting HDAC1 enzyme, and can be used for preparing anti-tumor medicines.

Description

technical field [0001] The invention relates to an effective part of rattan vine for inhibiting HDAC1 enzyme, a preparation method and an application, and belongs to the technical field of plant extracts. Background technique [0002] Broad vine ( Tinospora sinensis ) Tinospora sinensis Merr. of the genus Tinobilis of the family Fangjiceae is mostly used as medicine with canes. [See: Luo Dashang. Zhonghua Tibetan Materia Medica (Chinese) [M]. Minzu Publishing House, 1997.], is a traditional medicinal plant in Tibetan medicine, and the name of Tibetan medicine is "Lezhe". It is mainly born on hillsides and forests at an altitude of 900 meters. Distributed in Medog, Bomi, Guangdong, Hainan, Guangxi and Yunnan in my country's Tibet. [See: State Administration of Traditional Chinese Medicine "Chinese Materia Medica" Editorial Board. Chinese Materia Medica (Tibetan Medicine Volume) [M]. Shanghai Science and Technology Press, 2002.12:286]. "Jingzhu Materia Medica" records: Lez...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/59A61P35/00
CPCA61K36/59A61K2236/333A61K2236/39A61K2236/55
Inventor 芦永昌吴疆林鹏程钱帅王庶张海霞白雪郑虹朱奎德曾擎屹
Owner QINGHAI UNIV FOR NATITIES
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