Tinospora-sinensis anti-HiV-1-virus effective part and preparing method and application

A technology of effective parts and broad tendon vine resistance, which is applied in the direction of antiviral agents, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of no anti-AIDS and AIDS drug efficacy limitations, and achieve convenient extraction and expansion Raw material channel, effect of cytotoxic enhancement

Active Publication Date: 2017-01-04
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing anti-HIV drugs Like other types of anti-HIV drugs, the efficacy of marketed AIDS drugs is limited as HIV-1 reverse transcriptase develops resistance mutations
In this field, the main functions of the broad tendon rattan are relaxing tendons and activating collaterals, expelling wind and relieving pain, but there is no relevant research on anti-AIDS.

Method used

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  • Tinospora-sinensis anti-HiV-1-virus effective part and preparing method and application
  • Tinospora-sinensis anti-HiV-1-virus effective part and preparing method and application
  • Tinospora-sinensis anti-HiV-1-virus effective part and preparing method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiments 1 to 3 are specific implementations of the anti-HIV-1 virus effective parts, preparation methods and applications of the broad tendon vine of the present invention, wherein embodiment 1 is the best embodiment.

[0032] Example 1

[0033] The preparation method comprises the following steps: pulverize the rattan vine, use an ethanol solution with a mass percentage concentration of 75% to 85% for ultrasonic extraction for 1 to 2 hours, filter to obtain an ultrasonic extraction solution and filter residue, and add the filter residue with a mass percentage concentration of 75% to 85%. 85% ethanol solution was refluxed twice for 0.5 to 1 hour each time to obtain a reflux extract, combined with the ultrasonic extraction and reflux extract, concentrated and dried to obtain an extract; the extract was dispersed with distilled water to obtain an extract dispersion, and then Sequentially use petroleum ether, ethyl acetate and n-butanol as solvents to extract the extra...

Embodiment 2

[0035] The preparation method comprises the following steps: pulverizing the rattan vine, using an ethanol solution with a mass percentage concentration of 85% to 95% for ultrasonic extraction for 2 to 3 hours, filtering to obtain an ultrasonic extraction solution and filter residue, adding the filter residue with a mass percentage concentration of 85% to 95% ethanol solution was refluxed and extracted 3 times, each time for 1 to 1.5 hours, to obtain a refluxed extract, combined with an ultrasonic extraction and a refluxed extract, concentrated and dried to obtain an extract; the extract was dispersed with distilled water to obtain an extract dispersion, and then Sequentially use petroleum ether, ethyl acetate and n-butanol as solvents to extract the extract dispersion to obtain the extracts of each solvent, and evaporate the solvents from the ethyl acetate extract and n-butanol extract respectively to obtain the ethyl acetate part of vine vine and broad tendon n-butanol parts....

Embodiment 3

[0037] The preparation method comprises the following steps: pulverize the rattan vine, use an ethanol solution with a mass percentage concentration of 65% to 75% for ultrasonic extraction for 0.5 to 1 hour, and filter to obtain an ultrasonic extraction solution and filter residue, and add the filter residue with a mass percentage concentration of 65% to 75% ethanol solution was refluxed once for 1.5 to 2 hours to obtain the reflux extract, combined the ultrasonic extract and the reflux extract, concentrated and dried to obtain the extract; the extract was dispersed with distilled water to obtain the extract dispersion, and then sequentially Using petroleum ether, ethyl acetate and n-butanol as solvents to extract the extract dispersion, to obtain the extracts of the solvents, the ethyl acetate extract and the n-butanol extract were evaporated to remove the solvent, to obtain the ethyl acetate part and the Broad tendon vine n-butanol part.

[0038] Performance Testing

[0039...

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Abstract

The invention discloses a tinospora-sinensis anti-HiV-1-virus effective part and a preparing method and an application, and belongs to the technical field of plant extract. The tinospora-sinensis anti-HiV-1-virus effective part is selected from a tinospora-sinensis acetic ether part or a tinospora-sinensis n-butyl alcohol part. The preparing method includes the steps that tinospora sinensis is smashed, 65%-95% by mass of an alcohol solution is added for extracting, the obtained extracted liquid is concentrated and dried, and extractum is obtained; the extractum is dispersed into distilled water, extractum dispersion liquid is obtained, then petroleum ether, acetic ether and n-butyl alcohol are used as a solvent respectively and used for extracting the extractum dispersion liquid, the obtained n-butyl alcohol extracted liquid is subjected to solvent volatilizing, and the tinospora-sinensis n-butyl alcohol part is obtained; the extracted residual extractum dispersion liquid is concentrated and dried, and a tinospora-sinensis aqueous-phase extracting part is obtained. An application of the tinospora-sinensis anti-HiV-1-virus effective part to preparing anti-HiV-1-virus medicine aspects is suitable for being developed into novel anti-AiDS medicine.

Description

technical field [0001] The invention relates to an anti-HIV-1 virus effective part of vine vine, a preparation method and an application, and belongs to the technical field of plant extracts. Background technique [0002] Broad vine ( Tinospora sinensis ) Tinospora sinensis Merr. of the genus Tinobilis of the family Fangjiceae is mostly used as medicine with canes. [See: Luo Dashang. Zhonghua Tibetan Materia Medica (Chinese) [M]. Minzu Publishing House, 1997.], is a traditional medicinal plant in Tibetan medicine, and the name of Tibetan medicine is "Lezhe". It is mainly born on hillsides and forests at an altitude of 900 meters. Distributed in Medog, Bomi, Guangdong, Hainan, Guangxi and Yunnan in my country's Tibet. [See: State Administration of Traditional Chinese Medicine "Chinese Materia Medica" Editorial Board. Chinese Materia Medica (Tibetan Medicine Volume) [M]. Shanghai Science and Technology Press, 2002.12: 286]. "Jingzhu Materia Medica" records: Le Zhe tastes s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/59A61P31/18
CPCA61K36/59A61K2236/15A61K2236/33A61K2236/333A61K2236/35A61K2236/39
Inventor 芦永昌胡秦林鹏程钱帅田静王欢
Owner QINGHAI UNIV FOR NATITIES
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