Microfluidic chip apparatus adopting high hydrophobic substrate for detecting subtype swine influenza

Abstract

The invention relates to a microfluidic chip apparatus adopting a high hydrophobic substrate for detecting subtype swine influenza, which belongs to the field of analysis and test. The application of a substrate which is used for the microfluidic chip for diagnosing subtype swine influenza and made of polydimethylsiloxane (PDMS) low in price and easy to process has a series of difficulties. The invention provides a scheme for solving the series of difficulties. A key point of the scheme is that the substrate is made of the PDMS with an original ecological surface, a hinge-type clamp with a micro ultrasonic transducer is manually tightened and positioned at a position nearby a test sample liquid flow terminal of the microfluidic chip, the interface tension is reduced by virtue of the ultrasonic waves; meanwhile, by utilizing the high absorption capacity of the PDMS for the ultrasonic waves, the ultrasonic intensity is rapidly attenuated in a short distance, so that interface tension difference is formed at two ends of the chip, the difference provides power for driving the test sample liquid flow to flow to the terminal along a hydrophobic capillary passage, and the power is synergistic with mechanical pumping power of a micro-pump contained in the structure.

Description

technical field [0001] The invention relates to a microfluidic chip device for subtype swine influenza detection using a strongly hydrophobic substrate. The device is a special device for diagnosing subtype swine influenza antigens based on antigen / antibody specific reactions, and belongs to the field of analysis and testing. Background technique [0002] For the technical background of multi-channel microfluidic subtype swine influenza diagnosis, please refer to CN 201110311127.1 and other invention patent applications. [0003] As far as the overall overview of microfluidic technology itself is concerned, you can refer to the monograph "Illustrated Microfluidic Chip Laboratory" published by the famous microfluidic expert Mr. Lin Bingcheng not long ago, which has been published by Science Press. The past, present, and future prospects of microfluidic technology, etc., have detailed and long-form discussions that go deep into specific details. [0004] So, let's talk about ...

AI Technical Summary

Problems solved by technology

[0007] But it's not that simple

[0008] First, this polydimethylsiloxane material, the material referred to by the acronym PDMS, is itself a strongly hydrophobic material. Microchannels are built on this material. If the microchannels are not targeted The modification operation of the surface of the channel, then, after the overall assembly is completed, that is, after the cover is covered, because the inner surface of the micro channel in the structure occupies most of the inner surface of the liquid flow channel, then the PDMS micro channel The strong hydrophobic characteristic of the inner surface of the channel is the decisive factor, which will make it very difficult for the polar liquid flow similar to the aqueous solution to pass through, and its flow resistance is so large that even ordinary micropumps are difficult to push. Of course, If the cover sheet also chooses to use the PDMS material, then the problem is basically the same, with little difference; therefore, in the prior art, it is necessary to modify and modify the inner surface of the microchannel on the PDMS material; then , is this modification operation for the inner surface of the PDMS microchannel very troublesome? That's not the problem. What constitutes a serious technical problem is another problem: the PDMS polymer molecules in the bulk phase of the PDMS material substrate have the characteristics of automatic diffusion and migration to the surface. The characteristics of polymer molecules diffusing and migrating to the surface automatically will make the modified state of the inner surface of the microchannel modified by the surface modification unable to maintain for a long enough time, and the microgroove after surface modification The maintenance time of the inner surface state of the channel is roughly only enough to complete the time required for the internal test experiment in the laboratory; in other words, the inner surface of the PDMS microchannel after surface modification or surface modification is formed after modification The surface state of the surface does not last long, but quickly tends to or changes back to the surface state before the surface modification, and returns to the original strongly hydrophobic surface state in a relatively short period of time. Then, just imagine, Can such microfluidic chips be produced in large quantities, stored in large quantities, and widely promoted? The answer is obvious, that is, impossible

[0016] Third, as mentioned above, the inner surface of the PDMS microchannel is strongly hydrophobic, and targeted surface chemical modification or surface chemical modification is difficult to last. It is effective to use it within a short period of time; if the relatively short expiration date has passed and it is still used forcibly, since the surface state is already close to the hydrophobic state, there must be a comparison between using the conventional micropump to drive the sample liquid flow. Large flow resistance, in this way, the sample liquid can only be forced to flow in the target direction by increasing the pumping power and pumping pressure of the micropump. Pumping pressure to pump the sample liquid flow will cause bubbling, puffing, twisting, and deformation of the microchannels at the sampling end of the substrate including the area near the sampling end, and, under such high pressure conditions, The microgroove and its periphery at the sample inlet and its vicinity are also prone to peeling between the substrate and the cover slip. In this case, the sample solution will enter between the substrate and the cover slip formed after the peeling. This actually leads to the damage of the microfluidic chip; of course, if the surface modification or the surface modification is not in place, it will also lead to the above-mentioned situation within the short customary validity period; In the case of simply using an external micropump for liquid flow drive, the above-mentioned problem always exists

As mentioned above, if no pre-operations such as surface modification or surface modification have been done at all, then the above-mentioned problem will be more serious, even if the microchannel bubbling at the injection end and its vicinity does not occur , puffing, twisting, deformation, and peeling between the substrate and the cover sheet, etc., just because the flow resistance is too large, the use of a high-pressure micropump may not be able to drive the sample liquid flow toward the terminal.

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