A pyridine-degrading strain a5 and its production agent and application
A technology for degrading bacteria and strains, applied in the field of microbial remediation of environmental pollution, to achieve low production costs, protection of the ecological environment, and good removal effects
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Embodiment 1
[0056] Isolation and identification of strain a5:
[0057] The enrichment matrix used to enrich the pyridine degrading strains was taken from the activated sludge in the wastewater biochemical treatment pond of the pharmaceutical factory of Wanbei Pharmaceutical Co., Ltd. in Anhui Province, and 5.0 g of the activated sludge was added to the basic salt medium, and 50 mg·· L -1 Pyridine, 30℃, 150rpm, culture for 7 days, transfer to the same medium with 5% of the inoculum volume, transfer 3 times in succession, dilute the enrichment solution gradually, take 10 -4 -10 -7 0.1mL each of the diluted enrichment solution is coated with 100mg·L -1 On the solid basal salt medium plate of pyridine, cultured at 30°C for 3 days, pick the single colony grown and inoculate it with 200 mg·L -1 Incubate in pyridine basal salt medium at 30°C and 180 rpm for 3 days to verify the degradation effect of the degrading bacterial solution.
[0058] The degradation of pyridine in the pyridine-containing basal...
Embodiment 2
[0064] Analysis of the growth of strain a5 with different salt concentrations:
[0065] Take 5 clean test tubes and add 2mL LB culture solution respectively, and use salt concentration of 0%, 1%, 2%, 3%, 4% to explore the influence of different salt concentration on the growth of pyridine degrading bacteria a5. The culture conditions were 30°C, 150r / min constant temperature shaker shaking culture, 24h after culture, the optical density of the culture solution at 600nm was measured with a spectrophotometer. The result is Figure 4 As shown, a5 can grow when the salt concentration is 0-1%, and increasing the salt concentration within this range has little effect on the growth of the bacteria, and reaches the highest peak at 1%. But with the further increase of the salt concentration, the growth of a5 showed a downward trend. When the salt concentration reached 4%, no bacteria grew.
[0066] Analysis of the growth of strain a5 with different pH:
[0067] Take 7 clean test tubes and ad...
Embodiment 3
[0071] Preparation of degrading bacterial agent of strain a5
[0072] 1) Inoculate the test tube solution of the degraded strain a5 to the logarithmic phase in 100 mL of fermentation medium at an inoculum of 0.5% by volume, and shake the culture to the logarithmic phase to obtain the fermentation strain;
[0073] 2) Inoculate the above-prepared fermented strains at an inoculum of 5% (v / v, based on the volume of the medium) into 500 liters of seeds with a filling volume of 70% (based on the volume of the fermentor, the same below) Cultivate in the medium of the tank and cultivate to the logarithmic growth phase to prepare the seed liquid;
[0074] 3) The seed liquid is cultivated in the culture medium of the production tank with a liquid volume of 70% at an inoculum amount of 9% (v / v, based on the volume of the medium, the same below).
[0075] 4) During the cultivation process of the seed tank and the production tank, the aeration volume of sterile air is 1:1.5, the stirring speed is ...
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