A method for reducing protein content in Burnet polysaccharide

Abstract

A method for reducing protein content of Sanguisorba officinalis polysaccharides comprises the steps of adding 0.5-2 g of chitosan oligosaccharide into 100 ml of distilled water, stirring well, and swelling for 12-15 hours to obtain chitosan oligosaccharide aqueous solution for use; adding 1.8-3.5 g of Sanguisorba officinalis polysaccharides into 100 ml of distilled water, and stirring well to obtain Sanguisorba officinalis polysaccharide aqueous solution for use; adding 15-25 ml of the chitosan oligosaccharide aqueous solution slowly into the Sanguisorba officinalis polysaccharide aqueous solution under continuous stirring to obtain a mixed liquid; standing the mixed liquid at 25-35 DEG C for 1.5-2.5 hours to allow for flocculation, separating with a centrifuge for 5-8 min, and filtering to remove flocculant to obtain separate; making the separate into lyophilized powder with a lyophilizing unit, which is low-protein Sanguisorba officinalis polysaccharides; the method enables the removal rate for proteins in Sanguisorba officinalis polysaccharides to be increased, with much polysaccharides retained and zero harmful solvent left.

Description

technical field [0001] The invention provides a method for reducing protein content in burnet polysaccharide. Background technique [0002] Burnet is a perennial herb of the Rosaceae Burnet genus. Its roots are rich in active substances such as tannins, flavonoids, anthocyanins, and polysaccharides. Water and fire burns and other diseases have played an important role in scientific research, traditional Chinese medicine and other fields in recent years. Burnet eucalyptus polysaccharide is a new type of α-glucosidase inhibitor, which can reduce blood sugar and blood fat, increase the body’s immunity and other physiological effects. However, the high protein content of Burnet eucalyptus polysaccharide extracted from Reduced, limiting the in-depth research, development and utilization of Burnet polysaccharides, while conventional methods for removing protein from plant polysaccharides, such as sevag method, trichloroacetic acid method, etc., the protein removal rate is general...

AI Technical Summary

Problems solved by technology

Burnet eucalyptus polysaccharide is a new type of α-glucosidase inhibitor, which can reduce blood sugar and blood lipids, increase body immunity and other physiological effects, but the high protein content of Burnet eucalyptus polysaccharide extracted from Reduced, which limits the in-depth research, development and utilization of Burnet polysaccharides, and the conventional plant polysaccharide protein removal methods, such as sevag method, trichloroacetic acid method, etc., the protein removal rate is generally lower than 80%, even if more than 80% of the polysaccharide loss The loss rate is also high, and the loss rate is usually above 30%, and there are disadvantages such as cumbersome operation, long time-consuming, and some methods have harmful solvent residues.