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Method for extracting pectin from shaddock peel through enzymolysis and ultrasonic waves

A technology of grapefruit peel and enzymatic hydrolysis, applied in the field of pectin extraction, can solve the problems of low extraction efficiency, difficult temperature control, time-consuming, etc., and achieve the effects of improving extraction rate, easy operation, and saving solvents

Inactive Publication Date: 2017-01-25
安徽旺润生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Acid extraction is widely used because of its simple operation, but it consumes energy, time and low extraction efficiency
In recent years, there have been researches on the process of extracting pectin from pomelo peel by using microwave-assisted acid extraction, but it is difficult to control the temperature in the operation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for extracting pectin from grapefruit peel using enzymatic ultrasonic waves, the steps are as follows:

[0022] (1) Pretreatment: Wash the grapefruit peel, drain the water, and dry at 60-80℃, then cut into small pieces, crush with a grinder, and pass through a 40-mesh sieve to obtain the grapefruit peel powder for later use;

[0023] (2) Extraction:

[0024] Enzyme treatment: Take 100g of grapefruit peel powder in step (1), add 800ml of purified water, adjust its temperature to 35℃ and pH to 5.5, add 1g of compound composed of cellulase and acid protease (1:1) Enzyme, enzymolysis for 2h, get enzymolysis solution;

[0025] Ultrasonic treatment: adjust the pH of the enzymolysis solution to 1.00 with hydrochloric acid, heat up to 80°C, and ultrasonically treat for 80 minutes at a power of 175W;

[0026] (3) Decolorization: Add activated carbon to the ultrasonic treatment solution obtained in step (2) for decolorization. The mass-volume ratio of activated carbon to the solu...

example 2

[0029] A method for extracting pectin from grapefruit peel by using enzymatic ultrasound, the steps are as follows:

[0030] (1) Pretreatment: Wash the grapefruit peel, drain the water, and dry at 60-80℃, then cut into small pieces, crush with a grinder, and pass through a 40-mesh sieve to obtain the grapefruit peel powder for later use;

[0031] (2) Extraction:

[0032] Enzyme treatment: take 100g of grapefruit peel powder in step (1), add 800ml of purified water, adjust its temperature to 55℃, pH to 6.5, add 3g of composite enzyme composed of cellulase and acid protease (4:1) , Enzymatic hydrolysis for 1h to obtain enzymatic hydrolysate;

[0033] Ultrasonic treatment: adjust the pH of the enzymolysis solution to 1.50 with hydrochloric acid, heat up to 90°C, and ultrasonically treat for 70 minutes at a power of 175W;

[0034] (3) Decolorization: Add activated carbon to the ultrasonic treatment solution obtained in step (2) for decolorization. The mass-volume ratio of activated carbon ...

Embodiment 3

[0037] A method for extracting pectin from grapefruit peel by using enzymatic ultrasound, the steps are as follows:

[0038] (1) Pretreatment: Wash the grapefruit peel, drain the water, and dry at 60-80℃, then cut into small pieces, crush with a grinder, and pass through a 40-mesh sieve to obtain the grapefruit peel powder for later use;

[0039] (2) Extraction:

[0040] Enzyme treatment: Take 100g of grapefruit peel powder in step (1), add 600ml of purified water, adjust its temperature to 45℃ and pH to 6.0, add 2g of composite enzyme composed of cellulase and acid protease (2.5:1) , Enzymatic hydrolysis for 1.5h to obtain enzymatic hydrolysis solution;

[0041] Ultrasonic treatment: adjust the pH of the enzymatic hydrolysate to 1.25 with hydrochloric acid, heat up to 85°C, and ultrasonically treat for 75 minutes at a power of 175W;

[0042] (3) Decolorization: Add activated carbon to the ultrasonic treatment solution obtained in step (2) for decolorization. The mass-volume ratio of a...

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PUM

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Abstract

The invention discloses a method for extracting pectin from shaddock peel through enzymolysis and ultrasonic waves. The method includes the specific steps of (1) pretreatment of the shaddock peel serving as a raw material, (2), enzymolysis and ultrasonic treatment, (3), decoloration and (4), drying so as to obtain the pectin. The method for extracting the pectin from the shaddock peel through the enzymolysis and the ultrasonic waves has the advantages that a compound enzyme and the ultrasonic waves act on the shaddock peel jointly to extract the pectin from the shaddock peel; the enzyme is mild in action condition and plays a role in wall breaking, so that the pectin in the shaddock peel dissolves out thoroughly; an ultrasonic wave extraction process is a physical process without chemical change and is high in extraction rate and capable of protecting functional ingredients of extract effectively, breaking time is shortened, and the pectin yield of the shaddock peel is higher than 25% which is increased by more than 30% than that of a traditional acid extraction method; with the shaddock peel as the raw material, the method is simple, high in yield and high in extracted pectin quality and achieves the purpose of turning waste into wealth.

Description

Technical field [0001] The invention relates to a method for extracting pectin, in particular to a method for extracting pectin from grapefruit peel by using enzymatic hydrolysis and ultrasound. Background technique [0002] my country's grapefruit production and sales are very high, and grapefruit peel resources are very rich. At present, most of the grapefruit peel has not been rationally used except for a small amount of processing into candied products and medicinal purposes. Domestic and foreign studies have shown that grapefruit peel is rich in bioactive functional components such as pectin, flavonoids and essential oils, which has certain development value. Therefore, the development and research of the effective ingredients of grapefruit peel can not only avoid the waste of natural resources, reduce environmental pollution, but also promote the development of the grapefruit industry. Pectin is a natural high molecular polysaccharide polymer with good gelling, thickening...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/06
CPCC08B37/0045C08B37/0003
Inventor 曹秀玲其他发明人请求不公开姓名
Owner 安徽旺润生物科技有限公司
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