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A kind of preparation method of p-hydroxyphenylalanine urine detection reagent

A technology of p-hydroxyphenylalanine and hydroxyphenylalanine, which is applied in the field of preparation of p-hydroxyphenylalanine urine detection reagents, can solve the problem of low reliability of statistical results, inapplicability of tumor screening, The number of specimens to be detected is small, and the detection speed is fast, the cost is low, and the operation is simple.

Inactive Publication Date: 2021-02-26
程玉慧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reagents provided by the above-mentioned prior art have certain effects in application, high specificity and sensitivity, but there are still deficiencies. The reagents provided by Chinese patent CN1096374 have a high false positive rate, greater than 10%, and are not applicable Due to the general screening of tumors, the number of EP341803 specimens tested is small, so the reliability of the statistical results is not strong

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019] Preparation of liquid A: take ninhydrin and 80% ethanol solution according to the volume ratio of 1:3, mix evenly, and set aside; preparation of liquid B: take formic acid, ethylene glycol and silver nitrate at a molar ratio of 1:2:1.2, Stir evenly, then add ethylene glycol volume 30% sterile water to dilute to obtain a colorless transparent liquid for use; preparation of liquid C: take potassium iodide and azobisisobutylamidine hydrochloride according to the mass ratio of 10:1:3 and 1.2mol / L platinum nitrate solution, mix evenly, then add 30% sterile water of the mass of the mixture, and place it at 50°C for 20 minutes for later use; mix the above-prepared A liquid, the prepared B liquid and the prepared The C solution is mixed uniformly according to the volume ratio of 2:1.2:0.6, and the target product p-hydroxyphenylalanine urine detection reagent can be obtained.

[0020] Detection method: Take 8 mL of fresh morning urine after fasting in the morning and without usi...

example 2

[0024] Preparation of liquid A: take ninhydrin and 80% ethanol solution with a mass fraction of 1:3.5 according to the volume ratio, mix evenly, and set aside; preparation of liquid B: take formic acid, ethylene glycol and nitric acid at a molar ratio of 1:2.5:1.6 Silver, stir evenly, then add ethylene glycol volume 35% sterile water to dilute to obtain a colorless transparent liquid, for later use; C liquid preparation: according to the mass ratio of 13:1:4, take potassium iodide, azobisisobutylamidine salt Salt and 1.2mol / L platinum nitrate solution, mixed evenly, then added to sterile water with 32% mass of the mixture, and placed at 55°C for 25 minutes for later use; the prepared A liquid, the prepared B liquid and the prepared The C solution is mixed uniformly according to the volume ratio of 2.5:1.5:0.7, and the target product p-hydroxyphenylalanine urine detection reagent can be obtained.

[0025] Detection method: take 16mL of fresh morning urine after fasting in the m...

example 3

[0029] Preparation of liquid A: take ninhydrin and 80% ethanol solution according to the volume ratio of 1:4, mix evenly, and set aside; preparation of liquid B: take formic acid, ethylene glycol and silver nitrate at a molar ratio of 1:3:1.8, Stir evenly, then add ethylene glycol volume 40% sterile water to dilute to obtain a colorless transparent liquid, which is ready for use; preparation of liquid C: take potassium iodide and azobisisobutylamidine hydrochloride according to the mass ratio of 16:1:5 and 1.2mol / L platinum nitrate solution, mix evenly, then add 35% sterile water of the mass of the mixture, and place it at 60°C for 30 minutes for later use; prepare the prepared A liquid, the prepared B liquid and the prepared The liquid C is mixed uniformly according to the volume ratio of 3:1.8:0.9, and the target product p-hydroxyphenylalanine urine detection reagent can be obtained.

[0030] Detection method: take 20mL of fresh morning urine after fasting in the morning and...

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Abstract

The invention relates to an in vitro detection reagent for clinical diagnosis, in particular to a preparation method of a p-hydroxyphenylalanine urine detection reagent. The p-hydroxyphenylalanine urine detection reagent of the present invention contains silver ions, iodide ions, nitrate ions, platinum ions and azobisisobutylamidine hydrochloride, and is then prepared by mixing in proportion. The present invention is easy to sample and easy to operate. It is simple, can be used for large-scale general investigations of tumor metabolic abnormalities, etc., and has high spectral properties; the invention has high stability and sensitivity, can display specific colors and white precipitation phenomena, has very good recognition effects, and has a fast detection speed.

Description

technical field [0001] The invention relates to an in vitro detection reagent for clinical diagnosis, in particular to a preparation method of a p-hydroxyphenylalanine urine detection reagent. Background technique [0002] Cancer mortality ranks first among all diseases and is the number one killer of human health. It has become a major cause of death that threatens human life and health. According to statistics, the number of cancer patients will double every 25 years. Not only that, the number of deaths from cancer is also rising rapidly around the world, and this number may increase to 13.2 million in 2030. Early detection is the key to the treatment of tumors. The development of tumors is divided into five stages: benign, tumor cell initiation, early tumor, mid-tumor, and late stage. The frightening thing about cancer is that the first three stages cannot be detected by imaging. Even in the middle stage of the tumor, only very professional doctors can detect it with adv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/82
CPCG01N21/78G01N21/82
Inventor 程玉慧
Owner 程玉慧
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