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Method for detecting fragrance of silkworm chrysalis by headspace solid-phase microextraction and gas chromatography-mass spectrometry

A gas chromatography-mass spectrometry and silkworm chrysalis technology is applied in the field of headspace solid-phase microextraction-gas chromatography-mass spectrometry combined detection of silkworm chrysalis aroma, can solve the problems of less silkworm chrysalis volatile aroma components and species reports, and achieves high extraction speed and operating cost. Low, good spawn and accumulation effects

Inactive Publication Date: 2017-01-25
辽宁中北方正检测服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the current research on silkworm chrysalis involves many aspects and fields, there are few reports on the volatile aroma components and types produced by the secondary metabolism of silkworm chrysalis

Method used

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  • Method for detecting fragrance of silkworm chrysalis by headspace solid-phase microextraction and gas chromatography-mass spectrometry
  • Method for detecting fragrance of silkworm chrysalis by headspace solid-phase microextraction and gas chromatography-mass spectrometry
  • Method for detecting fragrance of silkworm chrysalis by headspace solid-phase microextraction and gas chromatography-mass spectrometry

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Embodiment 1

[0027] A method for detecting the aroma of silkworm chrysalis by headspace solid-phase microextraction-gas chromatography-mass spectrometry, comprising the following steps:

[0028] 1. Materials and equipment:

[0029] 1.1 Materials:

[0030] Fresh yellow silkworm chrysalis, fresh black silkworm chrysalis 250g each, Arowana refined first grade soybean oil.

[0031] 1.2 Instruments and equipment:

[0032] 7890GC-5975MS gas chromatography-mass spectrometry (Agilent, USA), 57330-U SUPELCO solid-phase microextraction handle, one-ten-thousandth balance (Sartorius Group, Germany), freeze dryer (Simon, USA), Haier ultra-low temperature refrigerator, induction cooker ,Water bath;

[0033] 2. Experimental method and content

[0034] 2.1 Pretreatment of silkworm chrysalis

[0035] 2.1.1 Aroma determination experiment of protein homogenate: Cut different kinds of silkworm chrysalis separately, remove their outer skin, take their protein, grind it with a mortar, weigh 2.00g each with...

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Abstract

The invention discloses a method for detecting fragrance of silkworm chrysalis by headspace solid-phase microextraction and gas chromatography-mass spectrometry. The method utilizes the fact that headspace solid-phase microextraction technology integrates sampling, extraction and enrichment, and has the advantages that extraction speed is high, operating cost is low, and there is no need for solvent extraction and infusion and other operations; automated analysis is implemented by the use with gas chromatography-mass spectrometry technology, the types and contents of characteristic flavor substances in the insect food material silkworm chrysalis, the effect of different nymphosis temperatures upon the types and formation of characteristic flavor matters in black and yellow fresh silkworm chrysalises are studied, and the influences of boiling, deep frying and lyophilization upon the flavor substances are compared in order to push the deep-processed silkworm chrysalises to the production and development of characteristic flavor seasonings; the method of the invention has a certain assistance in further researches on secondary metabolic process of insects, biological interaction of insects and animal and plant ecological mutual influence.

Description

technical field [0001] The invention relates to the technical field of chemical detection methods, in particular to a headspace solid-phase microextraction-gas chromatography-mass spectrometry method for detecting silkworm chrysalis aroma. Background technique [0002] Chromatography is a good means of separation, which can separate various components in complex mixtures, but its qualitative and structural identification capabilities are poor; mass spectrometry has a strong ability to identify the structure of unknown compounds, with high sensitivity, The detection is fast, but mass spectrometry itself cannot separate complex compounds, so high-purity samples are required for the identification of unknown compounds. Gas chromatography-mass spectrometry (GC-MS) first uses gas chromatography to effectively separate complex component samples, and obtains high-purity samples that just meet the requirements of mass spectrometry identification, with high resolution of chromatograp...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 白冰张瑶檀德宏张旋纪淑娟
Owner 辽宁中北方正检测服务有限公司
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