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Method of characterizing hydrogel microsphere surface morphology in wet in-situ condition

A technology of hydrogel microspheres and surface topography, applied in the field of materials, can solve the problems of surface structure and channel deformation, unable to maintain the shape and other problems, and achieve the effect of high Z-axis resolution

Active Publication Date: 2017-02-01
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its surface morphology can directly or indirectly affect the behavior of proteins and cells to determine its biocompatibility, but most of the current methods for characterizing the surface morphology of gel microspheres are scanning electron microscopy (SEM) or atomic force microscopy (AFM) after drying. ) scanning observation, the gel microspheres will lose water and shrink during the drying process, and the surface structure and pores will be deformed during the drying process, and cannot maintain the original shape in the wet state
The environmental scanning electron microscope (ESEM) can measure the surface morphology of the sample under certain water conditions, but because its wet mode must also be realized under certain temperature and vacuum conditions, it will inevitably lead to dehydration deformation of the gel microspheres.

Method used

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  • Method of characterizing hydrogel microsphere surface morphology in wet in-situ condition
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  • Method of characterizing hydrogel microsphere surface morphology in wet in-situ condition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Take a small amount of calcium alginate microspheres with a particle size of about 400 μm and place them in a flat plate.

[0022] 2. Use qualitative filter paper to absorb the free water adsorbed on the surface of the microspheres.

[0023] 3. Use a white light interferometer to measure the surface morphology and roughness of the microspheres. Place the sample on the sample stage of the white light interferometer. At room temperature and pressure, quickly locate the center of the microsphere within 10 minutes and fine-tune the objective lens. This process The middle CCD will record the interference fringes on the surface of the microspheres, and determine the statistical area as a circular range of 40 μm.

[0024] 4. According to the white light interferometer, the surface topography height of the gel microspheres is obtained, and the statistical result is 254.6nm. After further spherical aberration correction, the spherical aberration correction mode of the white l...

Embodiment 2

[0026] 1. Take a small amount of calcium alginate microspheres with a particle size of about 2 mm and place them in a flat plate.

[0027] 2. Use qualitative filter paper to absorb the free water adsorbed on the surface of the microspheres.

[0028] 3. Use a white light interferometer to measure the surface morphology and roughness of the microspheres. Place the sample on the sample stage of the white light interferometer. At room temperature and pressure, quickly locate the center of the microsphere within 5 minutes and fine-tune the objective lens. This process The middle CCD will record the interference fringes on the surface of the microspheres, and determine the statistical area as a circular range of 100 μm.

[0029] 4. Obtain the surface topography height of the gel microspheres according to the white light interferometer, and then further correct the spherical aberration, use the spherical aberration correction mode of the white light interferometry software to elimina...

Embodiment 3

[0031] 1. Take a small amount of alginic acid / chitosan microcapsules with a particle size of about 400 μm and place them in a flat plate.

[0032] 2. Use qualitative filter paper to absorb the free water adsorbed on the surface of the microspheres.

[0033] 3. Use a white light interferometer to measure the surface morphology and roughness of the microspheres. Place the sample on the sample stage of the white light interferometer. At room temperature and pressure, quickly locate the center of the microsphere within 10 minutes and fine-tune the objective lens. This process The middle CCD will record the interference fringes on the surface of the microspheres, and determine the statistical area as a circular range of 40 μm.

[0034] 4. According to the white light interferometer, the surface topography height of the gel microspheres is obtained, and the statistical result is 215.5nm. After further spherical aberration correction, the spherical aberration correction mode of the w...

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Abstract

The invention relates to a method of characterizing hydrogel microsphere surface morphology in a wet in-situ condition. The method comprises specific operation steps: gel microspheres are arranged in a plane dish in a single layer mode, free water on the surface of the microspheres is absorbed, white light interferometry is then used for measuring the microsphere surface morphology, a CCD quickly records interference fringes on the surface of the microspheres and determines a statistical range of microsphere surface roughness, the optical path difference between reference light and reflection light can be obtained according to strength changes of the interference fringes, the microsphere surface morphology height can be calculated accurately, and further, through spherical aberration correction to eliminate microsphere curvature influences, the real gel microsphere surface roughness can be obtained.

Description

technical field [0001] The invention relates to the field of materials, and relates to a method for characterizing the surface morphology of hydrogel microspheres in situ in a wet state. Background technique [0002] Hydrogel microspheres are currently widely used carriers in biomedical fields such as tissue engineering and drug release. Its surface morphology can directly or indirectly affect the behavior of proteins and cells to determine its biocompatibility, but most of the current methods for characterizing the surface morphology of gel microspheres are scanning electron microscopy (SEM) or atomic force microscopy (AFM) after drying. ) scanning observation, the gel microspheres will lose water and shrink during the drying process, and the surface structure and pores will be deformed during the drying process, and cannot maintain the original shape in the wet state. The environmental scanning electron microscope (ESEM) can measure the surface morphology of the sample un...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01B11/30G01B11/24G01B11/06
Inventor 马小军郑会珍于炜婷谢红国王锋任英
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI