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A wet in-situ method for characterizing the surface morphology of hydrogel microspheres

A technology of hydrogel microspheres and surface topography, which is applied in the field of materials, can solve the problems of surface structure and pore deformation, and cannot maintain shape, etc., and achieve the effect of high Z-axis resolution

Active Publication Date: 2019-01-25
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its surface morphology can directly or indirectly affect the behavior of proteins and cells to determine its biocompatibility, but most of the current methods for characterizing the surface morphology of gel microspheres are scanning electron microscopy (SEM) or atomic force microscopy (AFM) after drying. ) scanning observation, the gel microspheres will lose water and shrink during the drying process, and the surface structure and pores will be deformed during the drying process, and cannot maintain the original shape in the wet state
The environmental scanning electron microscope (ESEM) can measure the surface morphology of the sample under certain water conditions, but because its wet mode must also be realized under certain temperature and vacuum conditions, it will inevitably lead to dehydration deformation of the gel microspheres.

Method used

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  • A wet in-situ method for characterizing the surface morphology of hydrogel microspheres
  • A wet in-situ method for characterizing the surface morphology of hydrogel microspheres
  • A wet in-situ method for characterizing the surface morphology of hydrogel microspheres

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Experimental program
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Effect test

Embodiment 1

[0021] 1. Take a small amount of calcium alginate microspheres with a particle size of about 400 μm and place them in a flat plate.

[0022] 2. Use qualitative filter paper to absorb the free water adsorbed on the surface of the microspheres.

[0023] 3. Use a white light interferometer to measure the surface morphology and roughness of the microspheres. Place the sample on the sample stage of the white light interferometer. At room temperature and pressure, quickly locate the center of the microsphere within 10 minutes and fine-tune the objective lens. This process The middle CCD will record the interference fringes on the surface of the microspheres, and determine the statistical area as a circular range of 40 μm.

[0024] 4. According to the white light interferometer, the surface topography height of the gel microspheres is obtained, and the statistical result is 254.6nm. After further spherical aberration correction, the spherical aberration correction mode of the white l...

Embodiment 2

[0026] 1. Take a small amount of calcium alginate microspheres with a particle size of about 2 mm and place them in a flat plate.

[0027] 2. Use qualitative filter paper to absorb the free water adsorbed on the surface of the microspheres.

[0028] 3. Use a white light interferometer to measure the surface morphology and roughness of the microspheres. Place the sample on the sample stage of the white light interferometer. At room temperature and pressure, quickly locate the center of the microsphere within 5 minutes and fine-tune the objective lens. This process The middle CCD will record the interference fringes on the surface of the microspheres, and determine the statistical area as a circular range of 100 μm.

[0029] 4. Obtain the surface topography height of the gel microspheres according to the white light interferometer, and then further correct the spherical aberration, use the spherical aberration correction mode of the white light interferometry software to elimina...

Embodiment 3

[0031] 1. Take a small amount of alginic acid / chitosan microcapsules with a particle size of about 400 μm and place them in a flat plate.

[0032] 2. Use qualitative filter paper to absorb the free water adsorbed on the surface of the microspheres.

[0033] 3. Use a white light interferometer to measure the surface morphology and roughness of the microspheres. Place the sample on the sample stage of the white light interferometer. At room temperature and pressure, quickly locate the center of the microsphere within 10 minutes and fine-tune the objective lens. This process The middle CCD will record the interference fringes on the surface of the microspheres, and determine the statistical area as a circular range of 40 μm.

[0034] 4. According to the white light interferometer, the surface topography height of the gel microspheres is obtained, and the statistical result is 215.5nm. After further spherical aberration correction, the spherical aberration correction mode of the w...

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Abstract

The invention relates to a method for characterizing the surface morphology of hydrogel microspheres in situ in a wet state. The specific operation steps are arranging a single layer of gel microspheres in a flat dish, absorbing free water on the surface of the microspheres, and then using White light interferometry is used to measure the surface morphology of microspheres. CCD quickly records the interference fringes on the surface of microspheres to determine the statistical range of the surface roughness of microspheres. According to the change of the intensity of interference fringes, the optical path difference between the reference light and the reflected light can be obtained, and then it can be accurately Calculate the surface topography height of the microspheres, and then further correct the spherical aberration to eliminate the influence of the curvature of the microspheres to obtain the real surface roughness of the gel microspheres.

Description

technical field [0001] The invention relates to the field of materials, and relates to a method for characterizing the surface morphology of hydrogel microspheres in situ in a wet state. Background technique [0002] Hydrogel microspheres are currently widely used carriers in biomedical fields such as tissue engineering and drug release. Its surface morphology can directly or indirectly affect the behavior of proteins and cells to determine its biocompatibility, but most of the current methods for characterizing the surface morphology of gel microspheres are scanning electron microscopy (SEM) or atomic force microscopy (AFM) after drying. ) scanning observation, the gel microspheres will lose water and shrink during the drying process, and the surface structure and pores will be deformed during the drying process, and cannot maintain the original shape in the wet state. The environmental scanning electron microscope (ESEM) can measure the surface morphology of the sample un...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01B11/30G01B11/24G01B11/06
Inventor 马小军郑会珍于炜婷谢红国王锋任英
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI