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Test method and sample pretreatment method of flouroquinolone medicine in organic fertilizer

A fluoroquinolone and sample pretreatment technology, which is applied in the preparation of test samples, measuring devices, instruments, etc., can solve the problems of large measurement interference and complex components

Active Publication Date: 2017-02-01
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The composition of organic fertilizers is complex, which greatly interferes with the determination of fluoroquinolones. There is no suitable method for the determination of fluoroquinolones directly using organic fertilizers as samples. Therefore, it is necessary to establish a rapid method for the determination of fluoroquinolones in organic fertilizers. The determination method can be used for on-site detection of actual samples, and prevents organic fertilizer samples containing fluoroquinolones from reaching high and medium risks

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Take 0.5g organic fertilizer sample, add 10mL EDTA (pH=10.0) buffer solution, turn it upside down and shake for 8 minutes, let it stand until the upper layer is clear, take 2mL of the first supernatant, adjust the pH value to 4.0, add 6mL acetone, and shake again for 5 minutes , stand still until the upper layer is clarified, take 2mL of the second supernatant, add 50mg of magnetic PSA adsorbent, and take an appropriate amount of the third supernatant after solid-liquid separation under the action of a magnetic field and dry it for 2 minutes to obtain a transparent organic fertilizer pre- Process samples.

Embodiment 2

[0053] Take 0.5g organic fertilizer sample, add 10mL EDTA (pH=9.0) buffer solution, turn it upside down and shake for 8 minutes, let it stand until the upper layer is clear, take 2mL of the first supernatant, adjust the pH value to 3.5, add 6mL acetone, and shake again for 5 minutes , stand still until the upper layer is clarified, take 2mL of the second supernatant, add 50mg of magnetic PSA adsorbent, and take an appropriate amount of the third supernatant after solid-liquid separation under the action of a magnetic field and dry it for 2 minutes to obtain a transparent organic fertilizer pre- Process samples.

Embodiment 3

[0055] Take 0.5g organic fertilizer sample, add 10mL EDTA (pH=11.0) buffer solution, turn it upside down and shake for 8 minutes, let it stand until the upper layer is clear, take 2mL of the first supernatant, adjust the pH value to 4.5, add 6mL of acetone, and shake again for 5 minutes , stand still until the upper layer is clarified, take 2mL of the second supernatant, add 50mg of magnetic PSA adsorbent, and take an appropriate amount of the third supernatant after solid-liquid separation under the action of a magnetic field and dry it for 2 minutes to obtain a transparent organic fertilizer pre- Process samples.

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Abstract

The invention discloses a test method and a sample pretreatment method of flouroquinolone medicine in organic fertilizer. The sample pretreatment method comprises the steps of sampling organic fertilizer, adding EDTA buffer solution with 9.0-11.0 pH level, shaking thoroughly, waiting the solution turning static, taking a first supernatant liquid; adjusting the pH level of the first supernatant liquid to 3.5-4.5, adding organic solvent, shaking thoroughly again, waiting the solution turning static, taking out a second supernatant; the choice for the organic solvent shall select acetomitrile or acetone; adding adsorbing agent in the second supernatant liquid, shaking thoroughly, waiting the solution turning static, taking a third supernatant liquid and blowing dry, thus the pretreated sample of the organic fertilizer is obtained. The test method of flouroquinolone medicine in an organic fertilizer is to use the sample pretreatment method to obtain the pretreated sample of the organic fertilizer, namely the test sample, and directly applying one or more presently available test methods of flouroquinolone medicine for detection. The method has the advantages of simple, fast operation and high precision of test results which signifies enormous application potential.

Description

technical field [0001] The invention relates to the technical field of antibiotic detection, in particular to a method for measuring fluoroquinolones in organic fertilizers and a sample pretreatment method thereof. Background technique [0002] Organic fertilizers are carbon-containing materials mainly derived from plants and / or animals, and applied to the soil to provide plant nutrition as its main function. It is processed from biomass, animal and plant waste, and plant residues to eliminate toxic and harmful substances, and is rich in a large number of beneficial substances, including: a variety of organic acids, peptides, and rich nutrients including nitrogen, phosphorus, and potassium. nutritional elements. It can not only provide comprehensive nutrition for crops, but also has long fertilizer effect, can increase and renew soil organic matter, promote microbial reproduction, improve soil physical and chemical properties and biological activity, and is the main nutrien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N33/558G01N1/34
CPCG01N1/34G01N30/02G01N30/06G01N33/558
Inventor 钱鸣蓉杨华汪建妹刘艳平肖英平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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