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High density microalgae culture method in membrane photobioreactor using municipal wastewater

A photobioreactor, photobioreaction technology, applied in the direction of unicellular algae, bacteria, etc., can solve the problems of low nitrogen and phosphorus content, the water quality of effluent does not meet the discharge requirements, and it is difficult to maintain high-density culture of algal cells. The effect of production costs

Active Publication Date: 2017-02-15
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] However, compared with the algae medium, the nitrogen and phosphorus content of municipal sewage is relatively low. In the process of cultivating microalgae with municipal sewage, if the separation of algae and water cannot be achieved, it is difficult to provide sufficient and continuous nutrients for the growth of microalgae
And it is easy to take out the algae cells while draining the water, it is difficult to maintain the high-density cultivation of the algae cells in the reactor, and at the same time, the quality of the effluent water cannot meet the discharge requirements

Method used

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  • High density microalgae culture method in membrane photobioreactor using municipal wastewater
  • High density microalgae culture method in membrane photobioreactor using municipal wastewater
  • High density microalgae culture method in membrane photobioreactor using municipal wastewater

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Experimental program
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Effect test

Embodiment 1

[0042] The membrane photobioreactor structure that the present invention uses is as figure 1 Shown (with CN 204727884U in figure 1 The reactors are basically the same).

[0043]The membrane photobioreactor comprises a photobioreaction device 1, an illumination device 11, a gas supply device 9, a membrane permeation device 2, and a permeate collection device 13; the illumination device 11 is located outside the photobioreaction device 1; the light The upper part of the bioreactor 1 is provided with a sample inlet 7 and a gas outlet 8, the lower part is provided with a sample outlet 5 and a membrane module slot 6, and the bottom is provided with a microporous aeration tube 4; the gas supply device 9 communicates with light through a pipeline. The microporous aeration pipe 4 at the bottom of the bioreactor 1 is connected; the membrane permeation device 2 is installed in the photobioreaction device 1; the membrane permeation device 2 and the permeate collection device 13 are conn...

Embodiment 2

[0045] Example 2: Effect of Carbon Source Addition on the Growth of Spirulina and the Removal of Nutrients in Sewage

[0046] (1) The effect of carbon source addition on the biomass of Spirulina

[0047] The Spirulina platensis used in this example was purchased from the freshwater algae stock of the Institute of Hydrobiology, Chinese Academy of Sciences, numbered FACHB-901; the municipal sewage used was taken from the effluent of the primary sedimentation tank of Wuxi Taihu New Town Wastewater Treatment Plant, and the water quality indicators are shown in Table 1 shown.

[0048] Table 1 Municipal sewage water quality

[0049]

[0050] Changes in the biomass of microalgae during the process of cultivating microalgae with sewage figure 2 shown. figure 2 The results showed that the biomass of Spirulina continued to decrease in the raw sewage water without adding carbon source, and it grew to death on the third day, indicating that the raw sewage water could not be direct...

Embodiment 3

[0056] Embodiment 3: Determination of suitable hydraulic retention time in MPBR semi-continuous culture process

[0057] Inoculate Spirulina to add 1g / L NaHCO 3 In municipal sewage (see Table 1 for water quality indicators), when the density of MPBR algae reaches 1g / L-1.2g / L, semi-continuous culture is carried out, and the initial HRT is set to 7d. According to the daily growth of microalgae and the NH in sewage 4 + The removal of -N gradually reduces the HRT to 1.5d, maintaining the stable operation of the reactor. Among them, the inoculation density of algae cells is 0.5g / L, cultivated under the conditions of light intensity 3000Lux, temperature (30±1)°C, light-dark ratio 16 / 8.

[0058] (1) Changes of microalgae biomass, pH value and ammonia nitrogen in effluent under different HRT

[0059] The biomass changes of Spirulina in MPBR under different HRT as Figure 4 As shown, semi-continuous culture was started when the biomass reached 1.105g / L. When the HRT is 7d to 2d (t...

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Abstract

The invention discloses a high density microalgae culture method in membrane photobioreactor using municipal wastewater, belonging to the technical field of environment engineering and microalgae culture. In the method, municipal wastewater is added into the membrane photobioreactor, the algae cell is transplanted in a reactor, to achieve the purpose of culturing microalgae, and to achieve the culture effect of Zarrouk substrate at a basic level; the method reduces use of chemical medicines and plain water, and lowers the production cost of microalgae. Through filtration of the membrane components, the algae cell is entrapped in the reactor, high intensity culture is thus achieved; meanwhile the discharge of treated wastewater also achieves the treatment purpose of wastewater. In the process of microalgae culture, the method is able to save water resources and nutrition salt, the cost of microalgae culture is lowered, at the same time the wastewater is purified.

Description

technical field [0001] The invention relates to a method for cultivating microalgae with high density by using municipal sewage in a membrane photobioreactor, belonging to the technical fields of environmental engineering and microalgae cultivation. Background technique [0002] Algae cells contain protein, vitamins, trace elements and polysaccharides and other nutrients. Microalgae need to absorb and utilize a large amount of nutrients such as carbon, nitrogen and phosphorus in the process of growth and reproduction. How to reduce the use of nutrients and fresh water in the cultivation process is the key to realize the large-scale and commercial utilization of microalgae. [0003] Based on the high absorption rate of carbon, nitrogen and phosphorus by microalgae, the use of sewage to cultivate microalgae has been extensively studied in recent years. The supply of municipal sewage is large, and the domestic sewage pipe network system makes it easier to form a large-scale s...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/20
CPCC12N1/12C12N1/20
Inventor 任洪艳王雪飞阮文权
Owner JIANGNAN UNIV
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