Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Recombinant baculovirus for expressing dog serum albumin fused interferon gamma and application of recombinant baculovirus

A technology of recombinant baculovirus and serum albumin, which is applied in the field of mutation or genetic engineering, can solve the problem of large differences in the structure and function of natural proteins of veterinary interferon products, adverse reactions of animal immune tolerance, and the purification of veterinary interferon products Difficulties and other problems, to achieve the effect of fast and convenient fusion, simplified separation and purification work, and low cost

Inactive Publication Date: 2017-02-15
SOUTH CHINA AGRI UNIV
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used interferon for dog immune enhancement is conventional interferon. Repeated injections will cause immune tolerance and other adverse reactions in the animal body, which will affect its therapeutic effect.
[0008] 2. Most veterinary interferon products adopt prokaryotic expression, and the products are mostly in the form of inclusion bodies, and the preparation method is complicated
The purification process requires denaturation, renaturation and other processes, the biological activity of the product is poor, and the efficacy of the drug is general
[0009] 3. Veterinary interferon products differ greatly from natural proteins in structure and function
[0011] 5. It is difficult to purify veterinary interferon products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Recombinant baculovirus for expressing dog serum albumin fused interferon gamma and application of recombinant baculovirus
  • Recombinant baculovirus for expressing dog serum albumin fused interferon gamma and application of recombinant baculovirus
  • Recombinant baculovirus for expressing dog serum albumin fused interferon gamma and application of recombinant baculovirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0036] (1) Preparation of canine CSA-IFNγ fusion gene:

[0037] According to the canine serum albumin gene sequence (GenBank accession number: AB090854.1); the canine IFNγ gene sequence (GenBank accession number: EF095772.1) as the original sequence, according to the partial tropism of the insect cell expression system codon, optimize the synthesis of the new The nucleotide sequence of the canine interferon-γ mature peptide (the sequence was optimized and synthesized by Nanjing GenScript Biotechnology Co., Ltd.), and the synthetic primers were designed. The primer sequences are shown in Table 1.

[0038] Table 1 PCR primer sequence list

[0039]

[0040]

[0041]Step 1, the amplification method of canine serum albumin gene:

[0042] Take 0.5cm 3 Grind the canine liver sample from a cross grinder in an ice bath, collect the grinding solution in a 1.5mL centrifuge tube, freeze and thaw three times at -20°C, centrifuge at 12,000×g for 5min, collect the supernatant to extr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a recombinant baculovirus for expressing a dog serum albumin fused interferon gamma and application of the recombinant baculovirus. A dog serum albumin complete nucleotide sequence and a dog IFNgamma maturation protein nucleotide sequence are fused to construct a recombinant plasmid pF-CSA-IFNgamma for recombinant baculovirus screening; the recombinant baculovirus for stably expressing the dog serum albumin fused interferon gamma is screened. The recombinant baculovirus constructed through the method can stably secrete the novel dog gamma interferon with good expression activity and simple and convenient separation and purification, and lays a foundation for development of a novel dog long-acting interferon.

Description

technical field [0001] The invention relates to the technical field of mutation or genetic engineering, in particular to a recombinant baculovirus expressing canine serum albumin fusion interferon gamma and its application. [0002] technical background [0003] Interferon (Interferon, IFN) is a glycoprotein produced by viruses or other types of interferon inducers that stimulate the reticuloendothelial system, macrophages, lymphocytes and somatic cells. This protein has multiple biological activities, including anti-proliferative, immunomodulatory, antiviral and differentiation-inducing effects. Interferons are classified into type I and type II interferons based on specific receptors and sequence homology. IFN-γ is the only type II interferon, also known as immune interferon. Young (1996) and Frucht (2001) proposed that mainly by CD4 + Th1, CD8 + Secreted by cytotoxic T lymphocytes and NK cells, some B cells, NKT cells and APC cells can also secrete IFN-γ. Cantin (1999...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N7/01C07K19/00A61K38/21A61K47/64A61P37/04
CPCC07K14/57A61K38/217C07K2319/31
Inventor 罗满林李冰邹舒展张贺翟俊琼周霞
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com