Bimodal contrast agent, preparation method and application thereof

A dual-mode contrast agent and core technology, applied in the field of nanomedicine, can solve the problems that contrast agents cannot meet real-time monitoring and difficult to achieve deep imaging requirements, and achieve the effects of uniform size, regular appearance, and avoiding poor dispersion

Active Publication Date: 2020-11-17
TONGJI UNIV
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  • Description
  • Claims
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Problems solved by technology

Photoacoustic imaging is a new imaging method at present, which can improve the depth of optical imaging and the resolution of ultrasonic imaging to a certain extent. It is difficult to meet the clinical needs of deep imaging
[0003] In recent years, a single mode of contrast agent has been unable to meet the real-time monitoring of complex pathological conditions in clinic. In view of the complementarity of magnetic resonance imaging and photoacoustic imaging, the combination of the two has great application prospects and clinical significance

Method used

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  • Bimodal contrast agent, preparation method and application thereof
  • Bimodal contrast agent, preparation method and application thereof
  • Bimodal contrast agent, preparation method and application thereof

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Embodiment 1

[0030] This embodiment describes in detail the preparation methods of magnetic particles, sulfhydryl-modified magnetic balls and gold balls.

[0031] 1. Preparation of Magnetic Particles

[0032] FeCl 3 ·6H 2 Dissolve 4.1g (15.17mM) of O and 13.7g (45mM) of sodium oleate in a mixed solvent consisting of 40mL of ethanol, 60mL of water and 40mL of n-hexane, heat at 70°C for 4h, filter to obtain ferric oleate, and wash the ferric oleate with water Dry after 3 times. Then 3.6g (3mM) of dried ferric oleate and 1.6mL of oleic acid were dissolved in 20mL of octadecene, heated and reacted at 320°C for 1h under an argon atmosphere, and the obtained product was washed with toluene three times and then vacuum After drying, paramagnetic magnetic particles, namely oleic acid-modified iron ferric oxide nanoparticles are obtained. The particle size of the magnetic particles is 10nm-20nm as detected by the Malvern particle size analyzer.

[0033] 2. Preparation of thiol-modified magnetic...

Embodiment 2

[0043] Step S1, preparation of gold magnetic assembly balls: According to the mass ratio of 30:1, the gold balls and thiol-modified magnetic balls were mixed under ultrasonic conditions, stirred at room temperature for 24 hours to fully react, and centrifuged to obtain gold magnetic assembly balls , the product was washed with water three times, dispersed in water to obtain a mixture of gold magnetic assembly balls and water for use, wherein the ratio of gold magnetic assembly balls to water was 0.8g:1L.

[0044] Step S2, preparation of a dual-modal contrast agent with sea urchin structure: Add 600 μL of a mixture of gold magnetic assembly spheres and water, 600 μL of 1mol / L hydrochloric acid and 600 μL of 3 mmol / L AgNO 3 , vortexed and stirred for 10s to mix evenly; then 300 μL of 0.1mol / L ascorbic acid was added and vortexed for 60s, the solution changed from brownish yellow to dark green, and a solid was obtained after centrifugation, which was a dual-mode contrast agent wi...

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Abstract

The invention belongs to the technical field of nanomedicine, and particularly relates to a bimodal contrast agent, a preparation method and an application thereof.The preparation method comprises thesteps that a gold ball and a sulfydryl-modified magnetic ball are mixed and react to obtain a gold and magnetic assembly ball; then the gold and magnetic assembly ball and a chloroauric acid solutionare mixed, and vortex mixing is performed under the condition that the pH value is equal to 1-3; and AgNO3 and ascorbic acid are added for a vortex stirring reaction to obtain the bimodal contrast agent with the regular appearance and the uniform size. The bimodal contrast agent is of a sea urchin structure, an inner core is spherical ferroferric oxide particles, and an outer shell is acicular nanogold. The nanogold of the outer shell has relatively high absorbance in a first near-infrared region and a second near-infrared region, photoacoustic imaging can be achieved, and nuclear magnetic resonance imaging can be achieved through the magnetic nanoparticles of the inner core. The preparation method of the bimodal contrast agent provided by the invention has the characteristics of simple synthesis, low cost, simple and convenient operation and the like, and the obtained bimodal contrast agent can perform nuclear magnetic resonance imaging and photoacoustic imaging by utilizing existingequipment, and is beneficial to large-scale popularization.

Description

technical field [0001] The invention belongs to the technical field of nanomedicine, and in particular relates to a dual-mode contrast agent, a preparation method and an application thereof. Background technique [0002] With the development of imaging equipment and the development and application of contrast agents in clinical medicine, medical diagnosis can provide more accurate local details. Various medical imaging technologies have their own advantages and disadvantages in terms of temporal and spatial resolution. For example, nuclear magnetic resonance imaging has the advantages of large imaging depth, high spatial resolution, and good tissue contrast, but its sensitivity is slightly poor. There is no strict linear relationship with the contrast agent, and the imaging resolution is low, and the contrast agent is mostly a metal oxide, which is unstable in an acidic environment and is easily metabolized. Optical imaging has the characteristics of high sensitivity and hi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/12A61K49/18A61K49/22A61K49/00B82Y5/00B82Y15/00
CPCA61K49/126A61K49/186A61K49/225A61K49/0002B82Y5/00B82Y15/00
Inventor 成昱徐畅
Owner TONGJI UNIV
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