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Chitosan quaternary ammonium salt porcine acellular dermal matrix dressing material and preparation method thereof

A technology of chitosan quaternary ammonium salt and decellularized dermis, which is applied in the field of biomedical engineering, can solve the problems of susceptibility to bacterial infection, etc., and achieve the effect of lasting anti-infection ability, reducing pain and relieving pain

Inactive Publication Date: 2017-02-22
GUANGDONG TAIBAO MEDICAL SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention aims to solve the disadvantage that pig acellular dermal matrix is ​​easily infected by bacteria when used as a dressing, and provides a porcine acellular dermal matrix dressing of chitosan quaternary ammonium salt and a preparation method thereof

Method used

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  • Chitosan quaternary ammonium salt porcine acellular dermal matrix dressing material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A preparation method of chitosan quaternary ammonium salt pig acellular dermal matrix dressing, the specific steps of the preparation method are as follows:

[0024] S1, the preparation of chitosan quaternary ammonium salt: a certain amount of chitosan (amino content is n 1 ) was dissolved in glacial acetic acid solution with a mass concentration of 1% to make a chitosan solution, and then adjusted its pH to greater than 9 with 1 mol / L NaOH solution, so that the chitosan flocs were precipitated, and distilled water was added to stir for 1 hour. , Centrifuge at 3000rpm for 10 min to obtain chitosan precipitate, then add distilled water to immerse the precipitate and stir for 1 h to test the pH value of the water, and then wash the precipitate with distilled water until neutral. Put the chitosan precipitation in a round-bottomed flask, add isopropanol (the volume ratio of isopropanol to chitosan precipitation is 5:1), heat to 80°C, react for 60min, and then take the epoxy...

Embodiment 2

[0028] A preparation method of chitosan quaternary ammonium salt pig acellular dermal matrix dressing, the specific steps of the preparation method are as follows:

[0029] S1, the preparation of chitosan quaternary ammonium salt: a certain amount of chitosan (amino content is n 1 ) was dissolved in glacial acetic acid solution with a mass concentration of 1% to make a chitosan solution, and then adjusted its pH to greater than 9 with 1 mol / L NaOH solution to precipitate chitosan floccules. After adding distilled water and stirring for 1 h, Centrifuge at 3000 rpm for 10 min to obtain a chitosan precipitate, then add distilled water to immerse the precipitate and stir for 1 h to test the pH value of the water, and then wash the precipitate with distilled water until neutral. Put the chitosan precipitation in a round-bottomed flask, add isopropanol (the volume ratio of isopropanol to chitosan precipitation is 5:1), heat to 75°C, react for 30min, and then take the epoxy group-con...

Embodiment 3

[0032] Example 3: Animal Wound Healing Experiment

[0033] A total of 40 male New Zealand rabbits, each weighing 2-3 kg, were randomly divided into four groups with 10 rabbits in each group. All rabbits were created with deep second-degree wounds left by surgery according to the standard preparation method of clinical animal experimental burn models. The wounds of group A were sutured and then bandaged with clean gauze, and the gauze was changed every three days. The rabbits of group B The wound was sutured first, and then the porcine acellular dermal matrix was used as the dressing to wrap the wound. Groups C and D were respectively applied with the chitosan quaternary ammonium salt porcine acellular dermal matrix dressing of Examples 1 and 2 of the present invention, and did not need to be replaced during the test. The animal experiment was terminated until the wounds in groups C and D were healed.

[0034] Table 1 Wound infection and healing of animals in each group (%)

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Abstract

The invention belongs to the technical field of biomedical engineering, and particularly relates to a chitosan quaternary ammonium salt porcine acellular dermal matrix dressing material and a preparation method thereof. The preparation method of the chitosan quaternary ammonium salt porcine acellular dermal matrix dressing material specifically comprises the following steps: S1, preparing a chitosan quaternary ammonium salt; S2, processing a porcine acellular dermal matrix; and S3, preparing the chitosan quaternary ammonium salt porcine acellular dermal matrix dressing material. The chitosan quaternary ammonium salt porcine acellular dermal matrix dressing material disclosed by the invention completely reserves morphological structures and components of cells, has the characteristic of low antigenicity, and meanwhile can induce fiber-forming cells and vascular endothelial cells with regeneration capacity to grow therein.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, in particular to a chitosan quaternary ammonium salt pig acellular dermal matrix dressing and a preparation method thereof. Background technique [0002] The skin is a barrier that maintains homeostasis and prevents microbial invasion. The ultimate goal of skin wound treatment is to rebuild or restore the skin barrier. A well-performing wound dressing can temporarily act as a barrier and provide an environment conducive to wound healing. An ideal wound dressing should have: ① good adhesion, permeability, and moisture evaporation rate similar to normal skin; ② can prevent bacterial invasion and limit bacterial colonization on the wound surface; ③ relieve pain; ④ have good wound dressing. Elasticity and flexibility, can cover the wound according to the body shape, suitable for different parts of the human body; ⑤ Effectively prevent the formation of paralysis scars and wound shrinkage after t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/40A61L15/46A61L15/42
CPCA61L15/28A61L15/40A61L15/42A61L15/46A61L2300/412A61L2400/04C08L5/08
Inventor 卢亢陈锦涛庄壮辉陈泰灜
Owner GUANGDONG TAIBAO MEDICAL SCI TECH
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