A method for extracting vitamin C from ephedra fruit
A vitamin, ephedra technology, applied in the direction of organic chemistry, etc., can solve the problems of limited and restricted ephedra fruit application and development, etc., and achieve the effects of improving the purification rate, the cell fragmentation rate, and the impurity removal rate.
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Embodiment 1
[0027] A method for extracting vitamin C from ephedra fruit, comprising the following steps:
[0028] 1) Wash and sterilize the air-dried ephedra fruit first, crush it into 120 meshes, add 65% ethanol which is 7 times the weight of the ephedra fruit, stir, stir for 8 minutes, grind with a colloid mill for 35 minutes, and then adjust the temperature of the feed liquid to 45°C , the pH is 5.5, while adding 0.05% of ephedra fruit weight compound enzyme enzymolysis 2.5h, wherein, the compound enzyme is mixed by 4 parts by weight of cellulase, 3 parts by weight of pectinase and 0.3 parts by weight of protease Finally, keep the enzymolysis solution at 95°C for 7 minutes to sterilize, then place it at an ultrasonic frequency of 45kHz and a temperature of 35°C for ultrasonic extraction, extract for 18 minutes, and filter to obtain filtrate 1 and filter residue 1, which are set aside;
[0029] 2) Add 80% ethanol that is 8 times the weight of the filter residue 1 to the filter residue 1...
Embodiment 2
[0038] A method for extracting vitamin C from ephedra fruit, comprising the following steps:
[0039] 1) Wash and sterilize the air-dried ephedra fruit first, crush it into 100 meshes, add 60% ethanol which is 6 times the weight of the ephedra fruit, stir, stir for 5 minutes, grind with a colloid mill for 30 minutes, and then adjust the temperature of the feed liquid to 40°C , the pH is 5, while adding 0.04% by weight of ephedra fruit compound enzyme enzymolysis 2h, wherein, the compound enzyme is mixed by 3 parts by weight of cellulase, 2 parts by weight of pectinase and 0.2 parts by weight of protease , and finally keep the enzymolysis solution at 90°C for 6 minutes to sterilize, place it at an ultrasonic frequency of 40kHz and a temperature of 30°C for ultrasonic extraction, extract for 15 minutes, and filter to obtain filtrate 1 and filter residue 1, which are set aside;
[0040] 2) Add 70% ethanol that is 6 times the weight of the filter residue 1 to the filter residue 1 ...
Embodiment 3
[0049] A method for extracting vitamin C from ephedra fruit, comprising the following steps:
[0050] 1) Wash and sterilize the air-dried ephedra fruit first, crush it into 120 meshes, add 70% ethanol that is 8 times the weight of the ephedra fruit, stir, stir for 10 minutes, grind it with a colloid mill for 40 minutes, and then adjust the temperature of the feed liquid to 50°C , the pH is 6, while adding 0.06% of ephedra fruit weight compound enzyme enzymolysis 3h, wherein, the compound enzyme is mixed by 5 parts by weight of cellulase, 4 parts by weight of pectinase and 0.4 parts by weight of protease , and finally keep the enzymolysis solution at 100°C for 8 minutes to sterilize, then place it at an ultrasonic frequency of 50kHz and a temperature of 40°C for ultrasonic extraction, extract for 20 minutes, and filter to obtain filtrate 1 and filter residue 1, which are set aside;
[0051] 2) Add 90% ethanol that is 9 times the weight of the filter residue 1 to the filter resi...
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Abstract
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