Activity determination buffer solution and activity determination method of high-throughput Zika virus non-structural protease

Abstract

The invention discloses an activity determination buffer solution and an activity determination method of high-throughput Zika virus non-structural protease, wherein the buffer solution consists of the following buffer ingredients: 10-200mM of trihydroxymethyl aminomethane, 0-20mM of sodium chloride, 0-1mM of CHAPS, 0-20mM of galactose and glycerol which is 10-40% of in volume percentage, and the pH value of the buffer solution is at 8.0-9.0. The buffer solution provided by the invention can improve the activity of the protease in a process of detecting the enzymatic activity of Zika viruses, so that the high-throughput Zika virus non-structural protease activity determination method, which makes use of the buffer solution, is more accurate in result, and subsequently an effect of guiding the screening of Zika virus protease activity inhibitors is achieved and the research and development progress of medicines targeted to the Zika viruses is promoted.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to a high-throughput Zika virus non-structural protease activity assay buffer and an activity assay method. Background technique [0002] Zika virus (ZIKV) belongs to the Flaviviridae family (Fla.viviridae) and the genus Flavivirus. It is spherical in shape with a diameter of about 40-70 nm and has an envelope. Yellow fever virus belongs to the yellow fever virus genus of the yellow fever family. It is an RNA virus with viscerotropism and neurotropism. It is easy to die at room temperature. The genome is a single-stranded positive-strand RNA with a length of about 10.8Kb. The genome contains an open reading frame (ORF) encoding a polyprotein. [0003] Zika virus was first isolated in 1947 from macaques in the forests of Uganda. It was isolated from the human body for the first time in 1952. As of the end of the last century, Zika virus infection was thought to be distributed on...

AI Technical Summary

Problems solved by technology

Although most Zika virus-infected patients have mild symptoms and usually recover on their own after 3-7 days of onset, the medical community generally believes that this fast-spreading virus may be associated with an abnormally high number of newborns with microcephaly

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