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Molecular marker method of clematis heat-resistant gene site qHR1

A technology of molecular markers and gene loci, applied in the field of molecular genetics, can solve unseen problems

Inactive Publication Date: 2017-02-22
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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[0013] (1) Statistical analysis of heat resistance index of clematis

[0014] From June to September in 2014 and 2015, after three days of high temperature (T≥30°C), the heat damage symptoms of the test materials in the clematis nursery of the Institute of Botany, Chinese Academy of Sciences, Jiangsu Province were observed and recorded. The grading standard of heat damage symptoms refers to the literature (Kang Jungen, 2002) with slight changes. The performance (leaf color, new leaves, old leaves, flowers) and occurrence rules of clematis high temperature damage are systematically and comprehensively observed, and the observation items and progression It is: (1) Leaf color, grade 0: normal, no symptoms of heat damage; grade 1: a small part of leaves are yellow; grade 2: most leaves are yellow; grade 3: withered yellow and black. (2) degree of wilting of new leaves, 0 Grade: normal, no symptoms of heat damage; grade 1: new leaves wilt; grade 2: new leaves are partially dry; gra...

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Abstract

The invention provides a molecular marker method of a clematis heat-resistant gene site qHR1, and belongs to the field of molecular genetics. By virtue of 44 pairs of SSR molecular markers and 22 pairs of SRAP molecular markers, genotypes of 127 parts of clematis materials are determined, and in combination with heat resistance indexes, whole genome association and linkage analysis is conducted; through a GLM program and an MLM program, the clematis heat-resistant major gene site qHR1 is detected, 8.96% of phenotypic variation and 5.47% of phenotypic variation are interpreted, and an SSR molecular marker CNSSR80 is quite significantly correlated to the gene site. With the application of the molecular marker method provided by the invention, the problem of a slow progress in clematis heat-resistant breeding in China is solved, and moreover, the technical problems in an existing breeding technology which is high in breeding cost, long in duration, poor in stability and the like are solved, so that the breeding of a novel clematis heat-resistant variety in China can be accelerated.

Description

technical field [0001] The invention provides a molecular marker method for clematis heat-resistant gene site qHR1, belongs to the field of molecular genetics, and is specially used for directional cultivation of new heat-resistant clematis varieties. Background technique [0002] Clematis ( Clematis) It is a plant of Ranunculaceae (Ranunculaceae), a perennial woody vine. Clematis ornamental varieties have rich and varied flower shapes and colors, beautiful and luxuriant flowers, and the flowering period is from early spring to late autumn. "Queen" nickname. Clematis plants have occupied a very important position in the three-dimensional landscaping in Europe and other countries, and are also loved by the gardening circles and enthusiasts in my country. However, most of the clematis cultivars are not heat-resistant, and the high temperature in summer can easily cause the young leaves of clematis to wilt, deform the flowers, and shorten the flowering period. one of the ...

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 李林芳马育珠王鹏杨如同李亚汪庆王淑安
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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