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A kind of copper ion chromogenic reagent and its preparation method and application

A chromogenic reagent and copper ion technology, applied in the field of ion detection, can solve the problems of low cost, cumbersome operation steps, limited use range, etc.

Active Publication Date: 2019-05-10
厦门众仁合美生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above methods have their own advantages and disadvantages in terms of cost, operation complexity, detection sensitivity and selectivity.
For example, the commonly used sodium diethylaminodithioformate spectrophotometric method in the national standard has a low cost, but the operation steps are cumbersome and the detection sensitivity is relatively limited; while the high-sensitivity atomic absorption / emission spectrometry requires the help of relatively expensive price and operating costs. Large instruments, and highly qualified professional technicians, thus limiting their scope of use

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  • A kind of copper ion chromogenic reagent and its preparation method and application
  • A kind of copper ion chromogenic reagent and its preparation method and application
  • A kind of copper ion chromogenic reagent and its preparation method and application

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Embodiment Construction

[0030] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0031] Dissolve 3.32g of fluorescein in 10mL of methanol, then drop into 2.5mL of concentrated sulfuric acid as a catalyst, heat and reflux with a magnetic stirrer for about 14 hours, when the reaction stops, add 10g of ice, and then use 10g of NaHCO 3 Carefully neutralize the powder, filter, and then add 1% acetic acid solution to dissolve excess NaHCO in the reaction 3 , filtered, washed with water, and dried to obtain 2.946 g of fluorescein methyl ester.

[0032] Dissolve 0.5 g of fluorescein methyl ester in 10 mL of anhydrous acetonitrile, add BrCH 2 (CH 2 ) 4 CH 2 Br 2.03mL, where, BrCH 2 (CH 2 ) 4 CH 2 The concentration of Br species is 13.2mmol / mL, and K 2 CO 3 1.7 g, heated and stirred in an oil bath at 90°C for 5 hours, the mixture was precipitated by saturated NaCl, filtered, and dried to obtain 1.97 g of 6-O-hexy...

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Abstract

The invention discloses a copper ion coloring reagent which reacts only with copper ions in solution. The copper ion coloring reagent has an absorption wavelength of about 516 nm, does not cause color reaction with other common metal ions, and can be used as a copper ion exclusive detection agent. The invention also provides its preparation method. The preparation method includes using the three-step synthesis reaction with fluorescein as the precursor, and the UV-visible spectrophotometric method for the concentration of the copper ion solution is established based on the reagent.

Description

technical field [0001] The invention relates to the technical field of ion detection, in particular to a copper ion color reagent and its preparation method and application. Background technique [0002] Copper ion is the third essential element in the human body after iron and zinc. It plays an important role in physiological processes such as bone formation, cellular respiration, hematopoietic function, prevention of cardiovascular diseases, and promotion of connective tissue formation. ; It is also a catalytic factor for concentrated metalloenzymes. Its content in the human body is very important to human health. Usually, as people age, the human body's demand for copper ions will increase, from 0.4-0.6mg in infants to 1.5-3mg in adults. If the balance of copper ions in human cells is out of balance, it may cause many neurodegenerative diseases such as Alzheimer's disease and prion disease. In addition, using the principle that heavy metal ions can denature proteins, cop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 高攀峰温小娟李莹傅海燕吴义诚卿会
Owner 厦门众仁合美生物科技有限公司