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A method for determining the content of tsnas in mainstream cigarette smoke

A technology for cigarette mainstream smoke and content, which is applied to the determination of specific TSNAs content in cigarette mainstream smoke and the inspection of harmful components in cigarette smoke. It can solve the problems of poor selectivity, difficult measurement, and complicated processing process, and achieve easy operation. , selectivity and sensitivity improvement, and the effect of increasing extraction efficiency

Active Publication Date: 2019-04-19
SICHUAN BRANCH OF CHINA TOBACCO
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Problems solved by technology

However, the sample pretreatment process of GC-TEA method is complicated and the selectivity is poor.
The current most popular LC-MS / MS method generally selects ammonium acetate aqueous solution for extraction, and then directly loads the sample for analysis. more difficult

Method used

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  • A method for determining the content of tsnas in mainstream cigarette smoke
  • A method for determining the content of tsnas in mainstream cigarette smoke
  • A method for determining the content of tsnas in mainstream cigarette smoke

Examples

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Embodiment Construction

[0036] Further description will be made below in conjunction with specific examples.

[0037] The instruments and reagents used in this example are: Waters Xevo TQ ultra-high performance liquid chromatography-tandem mass spectrometer (Waters, USA), equipped with an electrospray ionization source (ESI); VtexMixer 230VeU oscillator (Labnet, USA).

[0038] Formic acid is HPLC grade (concentration is 49-51%, German Sigma company); Acetonitrile, methanol are chromatographically pure (U.S. Thermo-Fisher company); TSNAs, TSNAs-d 4 (Purity: 98%; Isotopic purity: 99%, Toronto Research Chemicals Inc.), water is ultrapure water.

[0039] The present invention uses ammonium acetate aqueous solution as the extraction liquid, vortex oscillation extraction, matrix dispersion extraction and purification, deuterated internal standard quantification, UPLC-MS / MS measurement and other steps to measure TSNAs in mainstream smoke, which can be quickly, accurately and simultaneously detected The con...

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Abstract

The invention discloses a method for measuring content of TSNAs (tobacco-specific nitrosamines) in mainstream smoke of cigarettes. The method comprises steps as follows: smoke capturing, target extraction, purification, preparation of a standard stock solution and a standard working solution and measurement of ultra-high performance LC-MS / MS (liquid chromatography-mass spectrometry / mass spectrometry). Compared with a traditional GC-TEA detection method, the method has the advantages that the pretreatment process is simplified with the adoption of ammonium acetate extraction, vortex oscillation, filtration and sample introduction manners, and the analysis sensitivity is improved. Compared with an existing LC-MS / MS method, the method has the advantages that the oscillation time of the vortex oscillation method is obviously shorter than that of ultrasonic extraction or oscillation extraction, the extraction efficiency is improved, and the oscillation time is shortened by 6 times. The method has the advantages of being convenient to operate, quick, accurate and good in sensitivity and repeatability.

Description

technical field [0001] The invention belongs to the technical field of cigarette smoke analysis, and in particular relates to the detection of harmful components in cigarette smoke, in particular to a method for measuring the content of specific TSNAs in mainstream smoke of cigarettes. Background technique [0002] Tobacco-specific TSNAs (TSNAs) mainly include N-nitrosonornicotine (NNN), 4-(methyl TSNAsyl)-1-(3-pyridyl)-1-butanone (NNK), N-nitroso N-nitroso aneunicotine (NAT) and N-nitrosonicotine (NAB). The International Agency for Research on Cancer (IARC), the Hecht research team, etc., the results of the toxicity evaluation of the harmful ingredients in the Canadian government list show that there is sufficient evidence that NNK in TSNAs is carcinogenic to laboratory animals. In recent years, the "Cigarette Hazard Index System Research" project organized and implemented by the State Tobacco Monopoly Administration also shows that NNK is one of the seven harmful chemical...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/88
CPCG01N30/02G01N30/06G01N30/88G01N2030/062
Inventor 熊巍韶济民庞夙陶晓秋黄玫张海燕杨雪
Owner SICHUAN BRANCH OF CHINA TOBACCO
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